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AAT Bioquest

Posted on October 25, 2022
What are the detection methods for western blot?
Enzyme-Linked Immunosorbent Assay (ELISA)Antibody and Protein LabelingImmunohistochemistry (IHC)Secondary AntibodiesWestern Blot


Posted on October 25, 2022
What are the steps involved in western blot?
Bioconjugation Enzyme-Linked Immunosorbent Assay (ELISA)Antibody and Protein LabelingHorseradish Peroxidase (HRP) and Poly-HRPSecondary AntibodiesWestern Blot


Posted on August 5, 2022
What are the differences between IgG, IgM, IgE and IgD?
Antibody and Protein LabelingSecondary AntibodiesImmunohistochemistry (IHC)Bioconjugation


Posted on September 29, 2021
What is secondary antibody cross reactivity?
Secondary AntibodiesAntibodies and Proteomics


Posted on September 28, 2021
What is the concentration of secondary antibodies for immunofluorescence?
Secondary AntibodiesAntibodies and ProteomicsImmunohistochemistry (IHC)Power Styramide™ Signal Amplification (PSA™)


Posted on September 28, 2021
What does cross adsorbed secondary antibody mean?
Secondary AntibodiesAntibodies and ProteomicsPrimary AntibodiesIndocyanine Green


Posted on September 28, 2021
How long do secondary antibodies last?
Secondary AntibodiesAntibodies and Proteomics


Posted on September 28, 2021
What is the role of secondary antibodies?
Secondary AntibodiesPrimary AntibodiesImmunohistochemistry (IHC)Enzyme-Linked Immunosorbent Assay (ELISA)


Posted on September 27, 2021
How do I know if an antibody is primary or secondary?
EnzymesSecondary AntibodiesAntibodies and ProteomicsHorseradish Peroxidase (HRP) and Poly-HRP


Posted on September 27, 2021
What does a conjugated antibody mean?
Horseradish Peroxidase (HRP) and Poly-HRPPE and APCSecondary AntibodiesAntibodies and Proteomics


Posted on September 27, 2021
What are the types of IgG?
Secondary AntibodiesAntibodies and Proteomics


Posted on September 27, 2021
What is the advantage of using the pre-adsorbed secondary antibody?
Secondary AntibodiesAntibodies and ProteomicsImmunohistochemistry (IHC)


Posted on September 24, 2021
What IgG class or subclass should I use for my primary antibody?
Protein to Protein ConjugationSecondary AntibodiesPrimary AntibodiesAntibody and Protein Labeling


Posted on September 24, 2021
What is pre-adsorption?
Secondary AntibodiesPE and APCBioconjugationProtein to Protein Conjugation


Posted on September 23, 2021
What is the difference between cross adsorbed/normal/purified antibodies?
Antibodies and ProteomicsSecondary Antibodies


Posted on September 23, 2021
What are the major benefits of using a secondary antibody
Antibodies and ProteomicsPrimary AntibodiesSecondary Antibodies


Posted on September 22, 2021
What label should I choose for a secondary antibody?
Secondary AntibodiesAntibodies and ProteomicsImmunohistochemistry (IHC)


Posted on September 22, 2021
What is the most important criterion for choosing a secondary antibody?
Secondary AntibodiesAntibodies and Proteomics


Posted on September 22, 2021
What are the common applications for secondary antibody conjugates?
Secondary AntibodiesBioconjugationImmunohistochemistry (IHC)Enzyme-Linked Immunosorbent Assay (ELISA)Antibody and Protein Labeling


Posted on September 22, 2021
How do I choose secondary antibodies?
Secondary AntibodiesAntibodies and ProteomicsHorseradish Peroxidase (HRP) and Poly-HRPEnzyme-Linked Immunosorbent Assay (ELISA)


Posted on September 22, 2021
What is HRP conjugated secondary antibody?
EnzymesSecondary AntibodiesHorseradish Peroxidase (HRP) and Poly-HRPPower Styramide™ Signal Amplification (PSA™)


Posted on September 22, 2021
Why is an HRP conjugated secondary antibody used in western blotting?
Horseradish Peroxidase (HRP) and Poly-HRPEnzymesSecondary AntibodiesAntibodies and Proteomics


Posted on September 22, 2021
What are the major differences between primary and secondary antibodies?
Secondary AntibodiesPrimary AntibodiesAntibody and Protein Labeling


Posted on September 21, 2021
Is IgG a secondary antibody?
Secondary AntibodiesAntibodies and ProteomicsPower Styramide™ Signal Amplification (PSA™)


Posted on September 21, 2021
What do anti-human antibodies bind to?
Secondary AntibodiesAntibodies and Proteomics


Posted on September 21, 2021
Are there different IgG antibodies?
Antibodies and ProteomicsImmunohistochemistry (IHC)Secondary Antibodies


Posted on September 21, 2021
What do anti-human antibodies do?
Secondary AntibodiesAntibodies and ProteomicsHorseradish Peroxidase (HRP) and Poly-HRP


Posted on September 21, 2021
What is anti human IgG?
Secondary AntibodiesAntibodies and ProteomicsHorseradish Peroxidase (HRP) and Poly-HRP


Posted on September 20, 2021
What are IgG antibodies used for?
Antibodies and ProteomicsAntibody and Protein LabelingSecondary Antibodies


Posted on May 7, 2021
What is a biotinylated secondary antibody?
BioconjugationSecondary Antibodies


Posted on May 7, 2021
What does biotinylated mean?
Secondary Antibodies


Posted on June 1, 2020
What is the difference between direct and indirect ELISA?
Antibodies and Proteomics Enzyme-Linked Immunosorbent Assay (ELISA)Antibody and Protein LabelingDirect ELISAPrimary AntibodiesSecondary Antibodies


Posted on April 24, 2020
Why does a secondary antibody have to originate from a host species different than that of the primary antibody?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingImmunohistochemistry (IHC)Primary AntibodiesSecondary Antibodies


Posted on April 24, 2020
What are the criteria for choosing primary and secondary antibodies in western blotting?
Antibodies and ProteomicsAntibody and Protein LabelingPrimary AntibodiesSecondary AntibodiesWestern Blot


Posted on April 24, 2020
Can I use an HRP-conjugated primary antibody for western blot (with no secondary)?
Antibodies and Proteomics Bioconjugation Chemical ReagentsAntibody and Protein LabelingHRPHRP-labeled primary antibodyHorseradish Peroxidase (HRP) and Poly-HRPPrimary AntibodiesSecondary AntibodiesWestern Blotantibody conjugation


Posted on April 24, 2020
What is the difference between indirect and direct imaging?
Antibodies and ProteomicsAntibody and Protein LabelingIHCImmunohistochemistry (IHC)Primary AntibodiesSecondary Antibodiesimmunofluorescence


Posted on April 17, 2020
In IHC what should be incubation time for primary antibody and secondary antibody ?
Antibodies and ProteomicsAntibody and Protein LabelingImmunohistochemistry (IHC)Primary AntibodiesSecondary Antibodies


Posted on April 17, 2020
Can we co-incubate the primary and secondary antibodies at the same time for Western Blotting ?
Antibodies and ProteomicsAntibody and Protein LabelingPrimary AntibodiesSecondary AntibodiesWestern Blot