Annexin V | AAT Bioquest

Annexin V-iFluor® 350 conjugate

Fluorescence Detection: Excitation at 345 nm and emission at 450 nm.
Apoptosis Detection: Detects phosphatidylserine exposed on the cell surface.
Calcium-Dependent Binding: Annexin V binds phosphatidylserine in the presence of calcium.
Instrument Compatibility: Supports 355 nm laser and DAPI filter sets.
Applications: Suitable for apoptosis analysis by flow cytometry and fluorescence microscopy.

Catalog	Size	Price	Quantity
20070	100 tests	Price

Overview

Annexins are a family of proteins that bind to phospholipid membranes in the presence of calcium. Annexin V is a valuable tool for studying cell apoptosis. It is used as a probe to detect cells expressing phosphatidylserine on the cell surface, a feature found in apoptosis and other forms of cell death. There are a variety of parameters that can be used for monitoring cell viability. Annexin V-dye conjugates are widely used to monitor cell apoptosis by measuring the translocation of phosphatidylserine (PS). In apoptosis, PS is transferred to the outer leaflet of the plasma membrane. The appearance of phosphatidylserine on the cell surface is a universal indicator of the initial/intermediate stages of cell apoptosis and can be detected before morphological changes can be observed. This fluorescent Annexin V conjugate has spectral properties similar to Alexa Fluor® 350 (Alexa Fluor® 350 is the trademark of Invitrogen).

Citations

View all 6 citations: Citation Explorer

Cancer stem cells and somatic stem cells as potential new drug targets, prognosis markers, and therapy efficacy predictors in breast cancer treatment

Authors:

Pershina, Olga and Ermakova, Natalia and Pakhomova, Angelina and Widera, Darius and Pan, Edgar and Zhukova, Mariia and Slonimskaya, Elena and Morozov, Sergey G and Kubatiev, Aslan and Dygai, Alexander and others,

Journal:

Biomedicines (2021): 1223

Cancer Stem Cells and Somatic Stem Cells as Potential New Drug Targets, and Prognosis Markers, and Therapy Efficacy Predictors in Breast Cancer Treatment

Authors:

Skurikhin, Evgenii and Pershina, Olga and Ermakova, Natalia and Pakhomova, Angelina and Widera, Darius and Pan, Edgar and Zhukova, Mariia and Morozov, Sergey and Kubatiev, Aslan and Dygai, Alexander

Genetic Factors as the Basis of Sex Differences in Damage to Lung Endothelium and Regulation of Angiogenesis Cells in Modeling Pulmonary Emphysema in C57BL/6 Mice with Dyslipidemia and Hyperglycemia

Authors:

Skurikhin, EG and Pakhomova, AV and Pershina, OV and Ermakova, NN and Krupin, VA and Pan, ES and Sandrikina, LA and Putrova, OD and Zhukova, MA and Kurochkina, IV and others,

Journal:

Bulletin of Experimental Biology and Medicine (2021): 326--331

Pericytes and Smooth Muscle Cells Circulating in the Blood as Markers of Impaired Angiogenesis during Combined Metabolic Impairments and Lung Emphysema

Authors:

Pakhomova, AV and Pershina, OV and Ermakova, NN and Krupin, VA and Pan, ES and Putrova, OD and Khmelevskaya, ES and Vaizova, OE and Pozdeeva, AS and Dygai, AM and others,

Journal:

Bulletin of Experimental Biology and Medicine (2020): 334--340

Resources-Application Notes Phenotypic and Epigenetic Mechanism of Action Determinations of Histone Methylase and Demethylase Inhibitors using Digital Widefield Microscopy

Authors:

Larson, Brad and Banks, Peter

Journal:

Experimental Biology (2017)

References

View all 32 references: Citation Explorer

Gold fluorescent annexin A5 as a novel apoptosis detection tool

Authors:

Kurschus FC, Pal PP, Baumler P, Jenne DE, Wiltschi B, Budisa N.

Journal:

Cytometry A (2009): 626

Glycogen synthase kinase-3 and Omi/HtrA2 induce annexin A2 cleavage followed by cell cycle inhibition and apoptosis

Authors:

Wang CY, Lin YS, Su WC, Chen CL, Lin CF.

Journal:

Mol Biol Cell (2009): 4153

Evaluation of annexin V and Calcein-AM as markers of mononuclear cell apoptosis during human immunodeficiency virus infection

Authors:

Palma PF, Baggio GL, Spada C, Silva RD, Ferreira SI, Treitinger A.

Journal:

Braz J Infect Dis (2008): 108

Measurement of annexin V uptake and lactadherin labeling for the quantification of apoptosis in adherent Tca8113 and ACC-2 cells

Authors:

Hu T, Shi J, Jiao X, Zhou J, Yin X.

Journal:

Braz J Med Biol Res (2008): 750

Detection of apoptosis induced by new type gosling viral enteritis virus in vitro through fluorescein annexin V-FITC/PI double labeling

Authors:

Chen S, Cheng AC, Wang MS, Peng X.

Journal:

World J Gastroenterol (2008): 2174

Physical properties

Molecular weight	~36000
Solvent	Water

Spectral properties

Correction factor (260 nm)	0.83
Correction factor (280 nm)	0.23
Extinction coefficient (cm ^-1 M ^-1)	20000 ¹
Excitation (nm)	345
Emission (nm)	450
Quantum yield	0.95 ¹

Usage and storage

Intended use	Research Use Only (RUO)
Storage	Freeze (< -15 °C); Minimize light exposure

Instrument settings

Flow cytometer
Excitation	355 or 405 nm laser
Emission	450/40 nm filter
Instrument specification(s)	Pacific Blue channel

Fluorescence microscope
Excitation	DAPI filter set
Emission	DAPI filter set
Recommended plate	Black wall/clear bottom

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Page updated on October 2, 2026