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Annexin V-iFluor® 350 conjugate

The detection of binding activity of Annexin V-iFluor® 350 conjugate to phosphatidylserine in Jurkat cells. Jurkat cells were treated without (Green) or with 1 μM staurosporine (Red) in 37 ºC for 4 hours, and then labeled with Annexin V-iFluor® 350 conjugate for 30 minutes. Fluorescence intensity was measured using ACEA NovoCyte flow cytometer in Pacific Blue channel.
The detection of binding activity of Annexin V-iFluor® 350 conjugate to phosphatidylserine in Jurkat cells. Jurkat cells were treated without (Green) or with 1 μM staurosporine (Red) in 37 ºC for 4 hours, and then labeled with Annexin V-iFluor® 350 conjugate for 30 minutes. Fluorescence intensity was measured using ACEA NovoCyte flow cytometer in Pacific Blue channel.
Ordering information
Price ()
Catalog Number20070
Unit Size
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Additional ordering information
Telephone1-408-733-1055
Fax1-408-733-1304
Emailsales@aatbio.com
InternationalSee distributors
ShippingStandard overnight for United States, inquire for international
Physical properties
Molecular weight36000
SolventWater
Spectral properties
Correction Factor (260 nm)0.83
Correction Factor (280 nm)0.23
Extinction coefficient (cm -1 M -1)200001
Excitation (nm)345
Emission (nm)450
Quantum yield0.951
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12352200

OverviewpdfSDSpdfProtocol


Molecular weight
36000
Correction Factor (260 nm)
0.83
Correction Factor (280 nm)
0.23
Extinction coefficient (cm -1 M -1)
200001
Excitation (nm)
345
Emission (nm)
450
Quantum yield
0.951
Annexins are a family of proteins that bind to phospholipid membranes in the presence of calcium. Annexin V is a valuable tool for studying cell apoptosis. It is used as a probe to detect cells which have expressed phosphatidylserine on the cell surface, a feature found in apoptosis as well as other forms of cell death. There are a variety of parameters that can be used for monitoring cell viability. Annexin V-dye conjugates are widely used to monitor cell apoptosis through measuring the translocation of phosphatidylserine (PS). In apoptosis, PS is transferred to the outer leaflet of the plasma membrane. The appearance of phosphatidylserine on the cell surface is a universal indicator of the initial/intermediate stages of cell apoptosis and can be detected before morphological changes can be observed. This fluorescent Annexin V conjugate has spectral properties similar to Alexa Fluor® 350 (Alexa Fluor® 350 is the trademark of Invitrogen).

Platform


Flow cytometer

Excitation355 nm laser
Emission450/50 nm filter
Instrument specification(s)Pacific Blue channel

Fluorescence microscope

Recommended plateBlack wall/clear bottom
Instrument specification(s)Pacific Blue channel

Calculators


Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of Annexin V-iFluor® 350 conjugate to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM2.778 µL13.889 µL27.778 µL138.889 µL277.778 µL
5 mM555.556 nL2.778 µL5.556 µL27.778 µL55.556 µL
10 mM277.778 nL1.389 µL2.778 µL13.889 µL27.778 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles
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Spectrum


Open in Advanced Spectrum Viewer
spectrum

Spectral properties

Correction Factor (260 nm)0.83
Correction Factor (280 nm)0.23
Extinction coefficient (cm -1 M -1)200001
Excitation (nm)345
Emission (nm)450
Quantum yield0.951

Citations


View all 6 citations: Citation Explorer
Cancer Stem Cells and Somatic Stem Cells as Potential New Drug Targets, and Prognosis Markers, and Therapy Efficacy Predictors in Breast Cancer Treatment
Authors: Skurikhin, Evgenii and Pershina, Olga and Ermakova, Natalia and Pakhomova, Angelina and Widera, Darius and Pan, Edgar and Zhukova, Mariia and Morozov, Sergey and Kubatiev, Aslan and Dygai, Alexander
Journal: (2021)
Genetic Factors as the Basis of Sex Differences in Damage to Lung Endothelium and Regulation of Angiogenesis Cells in Modeling Pulmonary Emphysema in C57BL/6 Mice with Dyslipidemia and Hyperglycemia
Authors: Skurikhin, EG and Pakhomova, AV and Pershina, OV and Ermakova, NN and Krupin, VA and Pan, ES and Sandrikina, LA and Putrova, OD and Zhukova, MA and Kurochkina, IV and others,
Journal: Bulletin of Experimental Biology and Medicine (2021): 326--331
Cancer stem cells and somatic stem cells as potential new drug targets, prognosis markers, and therapy efficacy predictors in breast cancer treatment
Authors: Pershina, Olga and Ermakova, Natalia and Pakhomova, Angelina and Widera, Darius and Pan, Edgar and Zhukova, Mariia and Slonimskaya, Elena and Morozov, Sergey G and Kubatiev, Aslan and Dygai, Alexander and others,
Journal: Biomedicines (2021): 1223
Pericytes and Smooth Muscle Cells Circulating in the Blood as Markers of Impaired Angiogenesis during Combined Metabolic Impairments and Lung Emphysema
Authors: Pakhomova, AV and Pershina, OV and Ermakova, NN and Krupin, VA and Pan, ES and Putrova, OD and Khmelevskaya, ES and Vaizova, OE and Pozdeeva, AS and Dygai, AM and others,
Journal: Bulletin of Experimental Biology and Medicine (2020): 334--340
Targeted Magnetic Intra-Lysosomal Hyperthermia produces lysosomal reactive oxygen species and causes Caspase-1 dependent cell death
Authors: Clerc, Pascal and Jeanjean, Pauline and Halalli, Nicolas and Gougeon, Michel and Pipy, Bernard and Carrey, Julian and Fourmy, Daniel and Gigoux, Véronique
Journal: Journal of Controlled Release (2017)

References


View all 32 references: Citation Explorer
Gold fluorescent annexin A5 as a novel apoptosis detection tool
Authors: Kurschus FC, Pal PP, Baumler P, Jenne DE, Wiltschi B, Budisa N.
Journal: Cytometry A (2009): 626
Glycogen synthase kinase-3 and Omi/HtrA2 induce annexin A2 cleavage followed by cell cycle inhibition and apoptosis
Authors: Wang CY, Lin YS, Su WC, Chen CL, Lin CF.
Journal: Mol Biol Cell (2009): 4153
Evaluation of annexin V and Calcein-AM as markers of mononuclear cell apoptosis during human immunodeficiency virus infection
Authors: Palma PF, Baggio GL, Spada C, Silva RD, Ferreira SI, Treitinger A.
Journal: Braz J Infect Dis (2008): 108
Detection of apoptosis induced by new type gosling viral enteritis virus in vitro through fluorescein annexin V-FITC/PI double labeling
Authors: Chen S, Cheng AC, Wang MS, Peng X.
Journal: World J Gastroenterol (2008): 2174
Measurement of annexin V uptake and lactadherin labeling for the quantification of apoptosis in adherent Tca8113 and ACC-2 cells
Authors: Hu T, Shi J, Jiao X, Zhou J, Yin X.
Journal: Braz J Med Biol Res (2008): 750
Two-way trafficking of Annexin V positive cells between mother and fetus: determination of apoptosis at delivery
Authors: Kolialexi A, Tsangaris GT, Anagnostopoulos A, Chondros D, Bagiokos V, Kitsiou S, Kanavakis E, Mavrou A.
Journal: Prenat Diagn (2007): 348
Rhodamine B isothiocyanate doped silica-coated fluorescent nanoparticles (RBITC-DSFNPs)-based bioprobes conjugated to Annexin V for apoptosis detection and imaging
Authors: Shi H, He X, Wang K, Yuan Y, Deng K, Chen J, Tan W.
Journal: Nanomedicine (2007): 266
Annexin A5-functionalized bimodal lipid-based contrast agents for the detection of apoptosis
Authors: van Tilborg GA, Mulder WJ, Deckers N, Storm G, Reutelingsperger CP, Strijkers GJ, Nicolay K.
Journal: Bioconjug Chem (2006): 741
Analysis of cycloheximide-induced apoptosis in human leukocytes: Fluorescence microscopy using annexin V/propidium iodide versus acridin orange/ethidium bromide
Authors: Baskic D, Popovic S, Ristic P, Arsenijevic NN.
Journal: Cell Biol Int (2006): 924
Quantum dots based probes conjugated to annexin V for photostable apoptosis detection and imaging
Authors: Le Gac S, Vermes I, van den Berg A.
Journal: Nano Lett (2006): 1863