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AAT Bioquest

Posted on April 10, 2024
What are the limitations of semi-dry transfer techniques for western blot?
Antibodies and Proteomics BioconjugationWestern Blot


Posted on April 10, 2024
What are the advantages of semi-dry transfer technique for western blot?
Antibodies and Proteomics BioconjugationWestern Blot


Posted on April 10, 2024
What are the advantages of tank transfer technique?
Antibodies and Proteomics BioconjugationWestern Blot


Posted on April 10, 2024
What are the limitations of tank transfer technique?
Antibodies and Proteomics BioconjugationWestern Blot


Posted on April 10, 2024
When should I use fluorescence detection methods for western blot?
Antibodies and Proteomics BioconjugationPrimary AntibodiesSecondary AntibodiesWestern Blot


Posted on April 10, 2024
What are the disadvantages of fluorescence detection method for western blot?
Antibodies and Proteomics BioconjugationFluorescencePrimary AntibodiesSecondary AntibodiesWestern Blot


Posted on April 10, 2024
What are the advantages of fluorescence detection methods for western blot?
Antibodies and Proteomics BioconjugationFluorescencePrimary AntibodiesSecondary AntibodiesWestern Blot


Posted on April 10, 2024
When should I use a chemiluminescent detection method for western blot?
Antibodies and Proteomics BioconjugationPrimary AntibodiesSecondary AntibodiesWestern Blot


Posted on April 10, 2024
What are the disadvantages of Chemiluminescent detection method for western blot?
Antibodies and Proteomics BioconjugationPrimary AntibodiesSecondary AntibodiesWestern Blot


Posted on April 10, 2024
What are the advantages of Chemiluminescent detection method for western blot?
Antibodies and Proteomics BioconjugationPrimary AntibodiesSecondary AntibodiesWestern Blot


Posted on April 10, 2024
When should I use colorimetric detection for western blot?
Antibodies and Proteomics BioconjugationPrimary AntibodiesSecondary AntibodiesWestern Blot


Posted on April 10, 2024
What are the disadvantages of colorimetric detection for western blot?
Antibodies and Proteomics BioconjugationPrimary AntibodiesSecondary AntibodiesWestern Blot


Posted on April 10, 2024
What are the advantages of colorimetric detection for western blot?
Antibodies and Proteomics BioconjugationPrimary AntibodiesSecondary AntibodiesWestern Blot


Posted on April 10, 2024
What are the most commonly used enzymes in colorimetric detection for western blot?
Antibodies and Proteomics BioconjugationPrimary AntibodiesSecondary AntibodiesWestern Blot


Posted on April 10, 2024
How do I choose the right western blot detection method?
Antibodies and Proteomics BioconjugationPrimary AntibodiesSecondary AntibodiesWestern Blot


Posted on April 10, 2024
What are the disadvantages of the radioactive detection method for western blot?
Antibodies and Proteomics BioconjugationPrimary AntibodiesSecondary AntibodiesWestern Blot


Posted on April 10, 2024
What are the advantages of the radioactive detection method for western blot?
Antibodies and Proteomics BioconjugationWestern Blot


Posted on April 10, 2024
What should I do if I have a high background (uniform distribution) in western blot?
Antibodies and Proteomics BioconjugationPrimary AntibodiesSecondary AntibodiesWestern Blotimmunofluorescence


Posted on April 10, 2024
How do I optimize the detection results with low molecular weight proteins?
Antibodies and Proteomics BioconjugationWestern Blotmolecular weight


Posted on April 10, 2024
What are the variables that need to be considered when optimizing protein transfer?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingWestern Blotimmunofluorescence


Posted on April 10, 2024
What roles do SDS and alcohol play in the western blot transfer step?
Antibodies and Proteomics BioconjugationPrimary AntibodiesSecondary AntibodiesWestern Blotimmunofluorescence


Posted on April 10, 2024
What are the different types of transfer methods in western blot?
Antibodies and Proteomics BioconjugationPrimary AntibodiesSecondary AntibodiesWestern Blotimmunofluorescence


Posted on April 10, 2024
Why is the data produced with a western blot typically considered to be semi-quantitative?
Antibodies and Proteomics BioconjugationPrimary AntibodiesSecondary AntibodiesWestern Blotimmunofluorescence


Posted on April 10, 2024
What are the applications of southern blotting?
Antibodies and Proteomics BioconjugationDNA and RNA QuantitationSecondary AntibodiesWestern Blotimmunofluorescence


Posted on April 10, 2024
What are the steps involved in southern blotting?
DNA and RNA QuantitationEnzymesGel ElectrophoresisPrimary AntibodiesSecondary AntibodiesWestern Blot


Posted on April 10, 2024
What are the principles of southern blotting?
Antibodies and Proteomics BioconjugationPrimary AntibodiesSecondary AntibodiesWestern Blotimmunofluorescence


Posted on April 10, 2024
What are the principles of western blotting?
Antibodies and Proteomics BioconjugationPrimary AntibodiesSecondary AntibodiesWestern Blotimmunofluorescence


Posted on April 10, 2024
What are the applications of western blotting?
Antibodies and Proteomics BioconjugationSecondary AntibodiesWestern Blotimmunofluorescence


Posted on April 10, 2024
What do I need for my western blot membrane blotting?
Antibodies and Proteomics BioconjugationSecondary AntibodiesWestern Blotimmunofluorescence


Posted on April 10, 2024
What are the disadvantages of nylon membranes?
Antibodies and Proteomics BioconjugationSecondary AntibodiesWestern Blotimmunofluorescence


Posted on April 10, 2024
What are the advantages of nylon membranes?
Antibodies and Proteomics BioconjugationSecondary AntibodiesWestern Blotimmunofluorescence


Posted on April 10, 2024
What are the differences between nitrocellulose membrane and nylon membrane?
Antibodies and ProteomicsPhysiological ProbesSecondary AntibodiesWestern Blotimmunofluorescence


Posted on April 10, 2024
How do I optimize my western blot experiments?
Antibodies and Proteomics BioconjugationSecondary AntibodiesWestern Blotimmunofluorescence


Posted on April 10, 2024
What are the common causes of an unexpected size of western blot band?
Antibodies and Proteomics BioconjugationSecondary AntibodiesWestern Blotimmunofluorescence


Posted on April 10, 2024
What should I take into consideration before I strip and reprobe my western blotting experiment?
Antibodies and Proteomics BioconjugationSecondary AntibodiesWestern Blotimmunofluorescence


Posted on November 7, 2023
What are the applications of immunochemical techniques?
Antibodies and Proteomics Bioconjugation Chemical Reagents Enzyme-Linked Immunosorbent Assay (ELISA)Cell SignalingCellular ProcessesImmunohistochemistry (IHC)Polymerase Chain Reaction (PCR)Western Blot


Posted on November 7, 2023
What are the most commonly used enzymes for protein detection?
Enzyme-Linked Immunosorbent Assay (ELISA)Biotin and StreptavidinHorseradish Peroxidase (HRP) and Poly-HRPWestern BlotProtein Assays & Analysis


Posted on June 15, 2023
What are the methods used to detect phosphorylated proteins?
Antibodies and ProteomicsEnzymesProteinWestern Blot


Posted on May 10, 2023
How do I measure kinase activity?
ATPCell SignalingCellular ProcessesEnzymesProteinWestern Blot


Posted on April 18, 2023
What types of labels can be attached to antibodies?
Antibodies and Proteomics Bioconjugation Enzyme-Linked Immunosorbent Assay (ELISA)Antibody LabelingAntibody and Protein LabelingImmunohistochemistry (IHC)Western Blot


Posted on March 1, 2023
What is the procedure of expression screening?
DNA and RNA QuantitationDNA and RNAGel ElectrophoresisPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)Western Blot


Posted on February 23, 2023
What are the different technologies available to analyze transcription factors?
DNA and RNA QuantitationDNA and RNAWestern Blot


Posted on October 25, 2022
What are the detection methods for western blot?
Enzyme-Linked Immunosorbent Assay (ELISA)Antibody and Protein LabelingImmunohistochemistry (IHC)Secondary AntibodiesWestern Blot


Posted on October 25, 2022
What are the steps involved in western blot?
Bioconjugation Enzyme-Linked Immunosorbent Assay (ELISA)Antibody and Protein LabelingHorseradish Peroxidase (HRP) and Poly-HRPSecondary AntibodiesWestern Blot


Posted on September 19, 2022
Why is SDS used in protein gel electrophoresis?
Agarose gel electrophoresisDNA and RNADNA sequencingGel ElectrophoresisPolymerase Chain Reaction (PCR)Western Blotdetergentgene sequencingpolyacrylamide gel


Posted on July 26, 2022
What is Cytokeratin 18?
Antibodies and Proteomics Enzyme-Linked Immunosorbent Assay (ELISA)Antibody LabelingImmunohistochemistry (IHC)Spectral Flow CytometryWestern Blotflow cytometryimmunocytochemistry


Posted on September 29, 2020
What is the difference between Western Blot and ELISA?
Antibodies and Proteomics Chemical Reagents Enzyme-Linked Immunosorbent Assay (ELISA)Antibody and Protein LabelingImmunohistochemistry (IHC)Western Blot


Posted on June 1, 2020
What is the difference between nitrocellulose and PVDF membranes in western blot?
Antibodies and ProteomicsGel ElectrophoresisProtein PurificationWestern Blot


Posted on June 1, 2020
What is enhanced chemiluminescence?
D-LuciferinHRPHorseradish Peroxidase (HRP) and Poly-HRPLuciferaseWestern Blotchemiluminescenceluciferin


Posted on June 1, 2020
Why are proteins transferred from gel to a membrane in western blot?
Gel ElectrophoresisProtein PurificationWestern Blot


Posted on June 1, 2020
What is the difference between SDS-PAGE and western blot?
Antibodies and ProteomicsGel ElectrophoresisProtein PurificationWestern Blot


Posted on April 24, 2020
What can I do if low molecular weight proteins are not showing any bands in western blot?
Antibodies and ProteomicsAntibody and Protein LabelingGel ElectrophoresisProteinWestern Blot


Posted on April 24, 2020
How can I determine if a protein has been knocked out?
Antibodies and ProteomicsProteinReal-Time PCR (qPCR)Western BlotqPCR


Posted on April 24, 2020
What are the criteria for choosing primary and secondary antibodies in western blotting?
Antibodies and ProteomicsAntibody and Protein LabelingPrimary AntibodiesSecondary AntibodiesWestern Blot


Posted on April 24, 2020
What is the major difference between Northern and Southern blotting?
Antibodies and ProteomicsAgarose gel electrophoresisDNA and RNAEnzymesGel ElectrophoresisNucleic AcidsProteinWestern Blotpolyacrylamide gel


Posted on April 24, 2020
What is an appropriate incubation time for primary antibody in western blot analysis?
Antibodies and ProteomicsAgarose gel electrophoresisAntibody and Protein LabelingGel ElectrophoresisWestern Blotpolyacrylamide gel


Posted on April 24, 2020
Can I use an HRP-conjugated primary antibody for western blot (with no secondary)?
Antibodies and Proteomics Bioconjugation Chemical ReagentsAntibody and Protein LabelingHRPHRP-labeled primary antibodyHorseradish Peroxidase (HRP) and Poly-HRPPrimary AntibodiesSecondary AntibodiesWestern Blotantibody conjugation


Posted on April 24, 2020
What is the difference between western blotting and immunoprecipitation?
Antibodies and ProteomicsGel ElectrophoresisProteinWestern Blot


Posted on April 24, 2020
What is the difference between Myc and c-Myc tag proteins?
Antibodies and Proteomics Enzyme-Linked Immunosorbent Assay (ELISA)Antibody and Protein LabelingImmunohistochemistry (IHC)ProteinWestern Blot


Posted on April 17, 2020
What are some reliable methods for validating antibodies?
Antibodies and Proteomics Enzyme-Linked Immunosorbent Assay (ELISA)Antibody and Protein LabelingIHCImmunohistochemistry (IHC)Western Blot


Posted on April 17, 2020
What is the role of Tau protein aggregation in neurodegeneration?
Antibodies and Proteomics Bioconjugation Enzyme-Linked Immunosorbent Assay (ELISA)Antibody and Protein LabelingWestern Blot


Posted on April 17, 2020
Can we co-incubate the primary and secondary antibodies at the same time for Western Blotting ?
Antibodies and ProteomicsAntibody and Protein LabelingPrimary AntibodiesSecondary AntibodiesWestern Blot


Posted on April 2, 2020
What is mitophagy in cell biology?
AutophagyMitochondriaWestern Blotmitochondrial DNACellular Processes


Posted on August 26, 2019
Why are cells lysed?
Cell AssayCell ViabilityWestern Blotcell lysishomogenouscentrifugeIHCSignal Amplificationantibody labelingimmunohistochemistry


Posted on August 21, 2019
Can I measure NADPH without lysing my cells?
NADH AssayNADP/NADPH ratioNADHNADPNADPHNADWestern Blotcell lysiscell assayemission wavelengthexcitation wavelengthfluorescence imagingfluorimetriclive cell analysislive cell assaymultiplex


Posted on July 8, 2019
What is a Western Blot?
ConjugationProteinagarose gelchemiluminescencecolorimetricfluorimetricWestern Blotprotein mass