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iFluor™ Labeling Probes

AAT Bioquest is rapidly expanding our product lines to meet your constantly changing research needs. We have been developing fluorescent dyes to solve various limitations with the existing fluorescent labeling reagents while offering classic fluorescent labeling reagents with high purity and competitive price to help you to get more research done with less money.

iFluor® dyes are the products of our recent R&D efforts. They are a series of excellent fluorescent labeling dyes that span the full UV-visible and near IR spectrum. All the iFluor® dyes have excellent water solubility. Their hydrophilic property makes their protein conjugation readily performed in aqueous media, minimizing the use of organic solvents. The resulted conjugates are resistant to precipitation during storage. iFluor® dyes also have much better labeling performance than the classic fluorescent labeling dyes such as FITC, TRITC, Texas Red®, Cy3®, Cy5® and Cy7®. Some of our iFluor® dyes significantly outperform Alexa Fluor® labeling dyes on certain antibodies.

Key Features of iFluor® Fluorescent Labeling Dyes

  • Excellent water solubility
  • Available in a variety of fluorescence colors
  • Their conjugates exhibit more intense fluorescence than other spectrally similar conjugates of classic fluorescent dyes
  • More photostable than the classic fluorescent dyes
  • Absorption spectra match the principal output wavelengths of common excitation sources
  • Robust and highly fluorescent over a broad pH range

 

Table 1. iFluor® Dye Equivalents of Common Dyes

If you are using
Try this iFluor® dye
Alexa Fluor® 350, AMCA, DyLight™ 350 iFluor® 350
Alexa Fluor® 405, DyLight™ 405 iFluor® 405
Alexa Fluor® 488, Cy2®, FITC, DyLight™ 488 iFluor® 488
Alexa Fluor® 514 iFluor® 514
Alexa Fluor® 532 iFluor® 532
Alexa Fluor® 555, Cy3®, DyLight™ 550, TRITC iFluor® 555
Alexa Fluor® 594, DyLight™ 594, Texas Red® iFluor® 594
Alexa Fluor® 633, DyLight™ 633 iFluor® 633
Alxea Fluor® 647, Cy5®, DyLight™ 650 iFluor® 647
Alexa Fluor® 680, Cy5.5®, IRDye® 700, DyLight™ 680 iFluor® 680

Table 2. Amine-Reactive iFluor® Dyes for Labeling Antibodies

Cat No.
Product Name
Ex (nm)
Em (nm)
Unit Size
Price
1020iFluor® 350 succinimidyl ester 3464431 mg$217
1021iFluor® 350 succinimidyl ester 4024251 mg$217
1023iFluor® 488 succinimidyl ester 4915181 mg$217
1024iFluor® 514 succinimidyl ester 5115271 mg$217
1025iFluor® 532 succinimidyl ester 5365601 mg$217
1028iFluor® 555 succinimidyl ester 5525671 mg$217
1029iFluor® 594 succinimidyl ester 5946141 mg$217
1038iFluor® 610 succinimidyl ester 6056271 mg$217
1030iFluor® 633 succinimidyl ester 6386551 mg$217
1030iFluor® 633 succinimidyl ester 6386551 mg$217

Table 3. Thiol-Reactive iFluor® Dyes for Labeling Antibodies

Cat No.
Product Name
Ex (nm)
Em (nm)
Unit Size
Price
1060iFluor® 350 maleimide 3464431 mg$217
1062iFluor® 488 maleimide 4915181 mg$217
1063iFluor® 555 maleimide 5525671 mg$217
1065iFluor® 647 maleimide 6496641 mg$217
1066iFluor® 680 maleimide 6766951 mg$217
1067iFluor® 700 maleimide 6857101 mg$217
1068iFluor® 750 maleimide 7497751 mg$217
1366iFluor® 790 maleimide 7828111 mg$327

 

iFluor® Dye-Labeled Antibodies


AAT Bioquest iFluor® dyes are optimized for labeling proteins, in particular, antibodies. These dyes are bright, photostable and have minimal quenching on proteins. They can be well excited by the major laser lines of fluorescence instruments (e.g., 350, 405, 488, 555 and 633 nm). The almost identical spectral characteristics to those of Alexa Fluor® and DyLight™ make iFluor®-labeled secondary antibody conjugates an excellent alternative to the anti-IgG conjugates of Alexa Fluor® and DyLight™. Secondary antibodies bind to the primary antibody to assist in detection, sorting and purification of target antigens. Our secondary antibodies are used throughout various types of assays, including ELISA or Western Blot. The types of the secondary antibodies are selected according to the source of the primary antibody, the class of the primary antibody (e.g., IgG or IgM), and the kind of label which is preferred.
 

Table 4. iFluor® Dye-Labeled Secondary Antibodies

Cat#
Product Name
Ex (nm)
Em (nm)
Size
Price
16440iFluor® 350 goat anti-mouse IgG (H+L)345442200 ug$84
16444iFluor® 405 goat anti-mouse IgG (H+L) 401420200 ug$84
16448iFluor® 488 goat anti-mouse IgG (H+L) 491514200 ug$84
16460iFluor® 555 goat anti-mouse IgG (H+L) 552567200 ug$84
16468iFluor® 594 goat anti-mouse IgG (H+L) 592614200 ug$84
16478iFluor® 633 goat anti-mouse IgG (H+L) 638655200 ug$84
16482iFluor® 647 goat anti-mouse IgG (H+L) 654674200 ug$84
16486iFluor® 680 goat anti-mouse IgG (H+L) 682701200 ug$84
16494iFluor® 700 goat anti-mouse IgG (H+L) 693713200 ug$84
16506iFluor® 750 goat anti-mouse IgG (H+L) 753779200 ug$84