iFluor™ Labeling Probes

AAT Bioquest is rapidly expanding our product lines to meet your constantly changing research needs. We have been developing fluorescent dyes to solve various limitations with the existing fluorescent labeling reagents while offering classic fluorescent labeling reagents with high purity and competitive price to help you to get more research done with less money.

iFluor^® dyes are the products of our recent R&D efforts. They are a series of excellent fluorescent labeling dyes that span the full UV-visible and near IR spectrum. All the iFluor^® dyes have excellent water solubility. Their hydrophilic property makes their protein conjugation readily performed in aqueous media, minimizing the use of organic solvents. The resulted conjugates are resistant to precipitation during storage. iFluor^® dyes also have much better labeling performance than the classic fluorescent labeling dyes such as FITC, TRITC, Texas Red^®, Cy3^®, Cy5^® and Cy7^®. Some of our iFluor^® dyes significantly outperform Alexa Fluor^® labeling dyes on certain antibodies.

Key Features of iFluor^® Fluorescent Labeling Dyes

• Excellent water solubility
• Available in a variety of fluorescence colors
• Their conjugates exhibit more intense fluorescence than other spectrally similar conjugates of classic fluorescent dyes
• More photostable than the classic fluorescent dyes
• Absorption spectra match the principal output wavelengths of common excitation sources
• Robust and highly fluorescent over a broad pH range

iFluor^® Dye-Labeled Antibodies

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AAT Bioquest iFluor^® dyes are optimized for labeling proteins, in particular, antibodies. These dyes are bright, photostable and have minimal quenching on proteins. They can be well excited by the major laser lines of fluorescence instruments (e.g., 350, 405, 488, 555 and 633 nm). The almost identical spectral characteristics to those of Alexa Fluor^® and DyLight™ make iFluor^®-labeled secondary antibody conjugates an excellent alternative to the anti-IgG conjugates of Alexa Fluor^® and DyLight™. Secondary antibodies bind to the primary antibody to assist in detection, sorting and purification of target antigens. Our secondary antibodies are used throughout various types of assays, including ELISA or Western Blot. The types of the secondary antibodies are selected according to the source of the primary antibody, the class of the primary antibody (e.g., IgG or IgM), and the kind of label which is preferred.