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AAT Bioquest

Posted on November 17, 2023
What are the principles of the TUNEL assay?
Apoptosis and NecrosisCaspasesDNA and RNADNA FragmentationDNA & RNA Markers and Ladders


Posted on October 10, 2023
What causes cells to stop growing?
Apoptosis and NecrosisCell Cycle AnalysisCell Cycle and ProlferationCell Cycle and ProliferationCell SenescenceDNA and RNA Quantitation


Posted on July 24, 2023
What are the precautions or limitations when using propidium iodide?
Chemical ReagentsApoptosis and NecrosisCell Viability AssaysDNA and RNANucleic AcidsPropidium iodide (PI)


Posted on July 24, 2023
Can propidium iodide be used in live cell imaging?
Chemical ReagentsApoptosis and NecrosisCell Viability AssaysDNA and RNANucleic AcidsPropidium iodide (PI)


Posted on July 24, 2023
What are the applications of propidium iodide?
Apoptosis and NecrosisCell Viability AssaysDNA and RNANucleic AcidsPropidium iodide (PI)


Posted on July 24, 2023
How is propidium iodide used in flow cytometry?
Chemical ReagentsApoptosis and NecrosisCell Viability AssaysDNA and RNANucleic AcidsPropidium iodide (PI)


Posted on July 24, 2023
How is propidium iodide typically applied to cells?
Chemical ReagentsApoptosis and NecrosisCell Viability AssaysFlow Cytometry ReagentsImmunohistochemistry (IHC)Nucleic AcidsPropidium iodide (PI)Spectral Flow Cytometry


Posted on June 27, 2023
How can cell viability assays be classified?
Chemical ReagentsApoptosis and NecrosisCell SignalingCell Viability AssaysCellular ProcessesLive or Dead


Posted on June 27, 2023
What are some common sources of error in cell viability assays?
Chemical ReagentsApoptosis and NecrosisCell SignalingCell Viability AssaysCellular ProcessesLive or Dead


Posted on June 27, 2023
What is the principle of cell viability assays?
Chemical ReagentsApoptosis and NecrosisCell SignalingCell Viability AssaysCellular ProcessesLive or Dead


Posted on June 27, 2023
What factors should I consider when selecting the right cell viability assay for my experiment?
Chemical ReagentsApoptosis and NecrosisCell SignalingCell Viability AssaysCellular ProcessesLive or Dead


Posted on June 27, 2023
What factors affect the accuracy of cell viability assays?
Chemical ReagentsApoptosis and NecrosisCell SignalingCell Viability AssaysCellular ProcessesLive or Dead


Posted on June 27, 2023
What are the limitations of cell viability assays?
Chemical ReagentsApoptosis and NecrosisCell SignalingCell Viability AssaysCellular ProcessesLive or Dead


Posted on June 27, 2023
What are the applications of cell viability assays?
Apoptosis and NecrosisCell SignalingCell Viability AssaysCellular ProcessesLive or Dead


Posted on June 27, 2023
How do cell viability assay work?
Apoptosis and NecrosisCell SignalingCell Viability AssaysCellular ProcessesChemical ReagentsLive or DeadPropidium iodide (PI)


Posted on June 22, 2023
What triggers entosis?
Apoptosis and NecrosisCell SignalingCell Viability AssaysCellular ProcessesPhysiological Probes


Posted on June 22, 2023
Can all cells undergo entosis?
Apoptosis and NecrosisCell Viability AssaysCellular ProcessesPhysiological Probes


Posted on June 22, 2023
How do cells within cells form?
Apoptosis and NecrosisCell Viability AssaysCellular ProcessesPhysiological Probes


Posted on June 22, 2023
What happens to a cell when it is internalized?
Apoptosis and NecrosisCell Viability AssaysCellular ProcessesPhysiological Probes


Posted on June 22, 2023
How does entosis differ from other types of cell engulfment?
Apoptosis and NecrosisCell Viability AssaysCellular ProcessesPhysiological Probes


Posted on June 22, 2023
Is entosis seen in vivo?
Apoptosis and NecrosisCell Viability AssaysCellular ProcessesPhysiological Probes


Posted on June 22, 2023
How might entosis affect tumors?
Apoptosis and NecrosisCell Viability AssaysCellular ProcessesPhysiological Probes


Posted on June 22, 2023
What is the mechanism of entosis?
Apoptosis and NecrosisCell Viability AssaysCellular ProcessesPhysiological Probes


Posted on June 22, 2023
What happens to the engulfed cell during entosis?
Apoptosis and NecrosisCell Viability AssaysCellular ProcessesPhysiological Probes


Posted on June 22, 2023
What is the difference between phagocytosis and pinocytosis?
Apoptosis and NecrosisCell Viability AssaysCellular ProcessesPhysiological Probesphagocytosis


Posted on June 22, 2023
What is the significance of pinocytosis?
Apoptosis and NecrosisCell Viability AssaysCellular ProcessesPhysiological Probes


Posted on June 22, 2023
What are the steps involved in pinocytosis?
Apoptosis and NecrosisCell Viability AssaysCellular ProcessesPhysiological Probes


Posted on June 22, 2023
What are the examples of pinocytosis?
Apoptosis and NecrosisCell Viability AssaysCellular ProcessesPhysiological Probes


Posted on June 22, 2023
What are the primary types of endocytosis?
Apoptosis and NecrosisCell Viability AssaysCellular ProcessesPhysiological Probesphagocytosis


Posted on June 22, 2023
What are the processes of pinocytosis?
Apoptosis and NecrosisCell Viability AssaysCellular ProcessesPhysiological Probes


Posted on June 22, 2023
What are the basic steps of phagocytosis?
Apoptosis and NecrosisCell Viability AssaysCellular ProcessesPhysiological Probes


Posted on June 22, 2023
What are the basic steps of endocytosis?
Apoptosis and NecrosisCell Viability AssaysCellular ProcessesCellular Structures and OrganellesPhysiological ProbesPlasma Membrane


Posted on June 22, 2023
What are the functions of exocytosis?
Apoptosis and NecrosisCell Viability AssaysCellular ProcessesPhysiological Probes


Posted on June 21, 2023
What is the principle of CVS assay?
Apoptosis and NecrosisCell CytotoxicityCell Viability AssaysCellular ProcessesDNAcell death


Posted on June 21, 2023
What is the principle of fluorometric cell viability assays?
Chemical ReagentsApoptosis and NecrosisCell CytotoxicityCell Viability AssaysCellular ProcessesPhysiological Probes


Posted on June 21, 2023
What is the principle of real-time viability assay?
Apoptosis and NecrosisCell SignalingCell Viability AssaysCellular ProcessesPropidium iodide (PI)


Posted on June 21, 2023
What is the principle of flow cytometric cell viability assays?
Apoptosis and NecrosisCell SignalingCell Viability AssaysCellular ProcessesFlow Cytometry ReagentsLive or Dead


Posted on June 21, 2023
What are the limitations of dye exclusion assays?
Chemical ReagentsApoptosis and NecrosisCell SignalingCell Viability AssaysCellular ProcessesLive or DeadPhysiological Probes


Posted on June 21, 2023
What are the limitations of colorimetric assays of cell viability and cytotoxicity?
Apoptosis and NecrosisCell CytotoxicityCell Viability AssaysCellular ProcessesLive or DeadPhysiological Probes


Posted on June 21, 2023
What are the limitations of fluorometric cell viability assays?
Apoptosis and NecrosisCell SignalingCell Viability AssaysCellular ProcessesFlow Cytometry ReagentsLive or Dead


Posted on June 21, 2023
What are the pros of membrane asymmetry cell viability assays?
Annexin-V StainingApoptosis and NecrosisCell Viability AssaysCellular ProcessesChemical ReagentsClassic Dyes


Posted on June 21, 2023
What are the cons of membrane asymmetry cell viability assays?
Chemical ReagentsAnnexin-V StainingApoptosis and NecrosisCell Viability AssaysCellular ProcessesClassic Dyes


Posted on June 21, 2023
What are the pros of membrane permeability assays?
Chemical ReagentsApoptosis and NecrosisCell SignalingCell Viability AssaysCellular ProcessesLive or DeadPhysiological Probes


Posted on June 21, 2023
What are the cons of membrane permeability assays?
Chemical ReagentsApoptosis and NecrosisCell SignalingCell Viability AssaysCellular ProcessesPhysiological Probes


Posted on June 21, 2023
What dyes have been used for the dye exclusion method?
Chemical ReagentsApoptosis and NecrosisCell Viability AssaysCellular ProcessesPhysiological Probes


Posted on June 21, 2023
What are the advantages of real-time viability assay?
Chemical ReagentsApoptosis and NecrosisCell Viability AssaysCellular Processes


Posted on June 21, 2023
What are the disadvantages of real-time viability assay?
Chemical ReagentsApoptosis and NecrosisCell Viability AssaysCellular Processes


Posted on June 21, 2023
What are the advantages of fluorometric assays of cell viability and cytotoxicity?
Chemical ReagentsApoptosis and NecrosisCell CytotoxicityCell SignalingCellular ProcessesLive or Dead


Posted on June 9, 2023
What triggers necrosis?
Apoptosis and NecrosisCaspasesCell Viability AssaysCellular Processescell death


Posted on June 9, 2023
How do cells die?
Apoptosis and NecrosisCaspasesCell Viability AssaysCellular ProcessesEnzymescell death


Posted on June 9, 2023
What are the major steps of apoptosis?
Apoptosis and NecrosisCaspasesCell Viability AssaysCellular ProcessesEnzymescell death


Posted on June 9, 2023
What signaling pathways trigger apoptotic cell death?
Apoptosis and NecrosisCell Viability AssaysCellular Processescell death


Posted on June 9, 2023
What are the characteristics of apoptosis?
Apoptosis and NecrosisCaspasesCell Viability AssaysCellular ProcessesEnzymescell death


Posted on June 9, 2023
What are the characteristics of necrosis?
Apoptosis and NecrosisCell Viability AssaysCellular Processescell death


Posted on June 9, 2023
What are the differences between autophagy and apoptosis?
Apoptosis and NecrosisCell Viability AssaysCellular Processescell death


Posted on June 9, 2023
What are the similarities between autophagy and apoptosis?
Apoptosis and NecrosisAutophagyCell Viability AssaysCellular Processescell death


Posted on June 9, 2023
What are the methods used to analyze cell death?
Annexin-V StainingApoptosis and NecrosisCell Viability AssaysCellular ProcessesDNAFlow Cytometry ReagentsPropidium iodide (PI)Spectral Flow Cytometrycell death


Posted on May 3, 2023
How do cells communicate?
Apoptosis and NecrosisCell Cycle AnalysisCell Cycle and ProliferationCell CytotoxicityCell MetabolismCell SignalingCell ViabilityCellular ProcessesG-Protein-Coupled Receptors (GPCR)Membrane Potential and Channels


Posted on May 3, 2023
What are the common outcomes of cell signaling?
Apoptosis and NecrosisCell Cycle AnalysisCell Cycle and ProliferationCell CytotoxicityCell MetabolismCell SignalingCellular ProcessesEnzymes


Posted on May 3, 2023
What are the cellular responses to the cell signaling pathway?
Apoptosis and NecrosisCell Cycle and ProliferationCell SignalingCellular ProcessesEnzymesProtein


Posted on April 13, 2023
How do I differentiate pro-caspases and caspases?
ApoptosisApoptosis and NecrosisCell Viability AssaysCellular Processes


Posted on April 13, 2023
What are the types of apoptotic signaling pathways?
BioconjugationApoptosisApoptosis and NecrosisCell Viability AssaysCellular Processes


Posted on April 13, 2023
What are the common stimuli for apoptosis?
ApoptosisApoptosis and NecrosisCell Viability AssaysCellular Processes


Posted on April 13, 2023
What is the role of caspases in apoptosis?
ApoptosisApoptosis and NecrosisCaspasesCell Viability AssaysCellular ProcessesEnzymes


Posted on April 13, 2023
What are the differences between autolysis and apoptosis?
ApoptosisApoptosis and NecrosisCell Viability AssaysCellular ProcessesEnzymes


Posted on April 13, 2023
What are the similarities between autolysis and apoptosis?
ApoptosisApoptosis and NecrosisCell Viability AssaysCellular Processes


Posted on January 26, 2023
What are the differences between reversible and irreversible cell injury?
Apoptosis and NecrosisCell SignalingCellular ProcessesCellular Structures and OrganellesEnzymescell damagecell death


Posted on January 26, 2023
What are the characteristics of irreversible cell injury?
ATPApoptosis and NecrosisCell SignalingCellular ProcessesCellular Structures and OrganellesEnzymesMitochondriacell damage


Posted on November 30, 2022
How do cells protect themselves against cellular stress?
Apoptosis and NecrosisCell Cycle AssaysCell Proliferation AssaysCell Structures and OraganellesCell Viability AssaysPhysiological Probes


Posted on November 17, 2022
What's the significance of caspase-9?
ApoptosisApoptosis and NecrosisCaspasesCell Viability AssaysCellular ProcessesEnzymes


Posted on November 17, 2022
Where are caspases found?
ApoptosisApoptosis and NecrosisCaspasesCell Viability AssaysCellular ProcessesEnzymes


Posted on November 17, 2022
Which enzyme is responsible for apoptosis?
ApoptosisApoptosis and NecrosisCaspasesCell Viability AssaysCellular ProcessesEnzymes


Posted on November 17, 2022
What are the types of caspases?
ApoptosisApoptosis and NecrosisCaspasesCell Viability AssaysCellular ProcessesEnzymes


Posted on November 17, 2022
What are the similarities between procaspase and caspase?
Apoptosis and NecrosisCaspasesCell Viability AssaysCellular ProcessesEnzymes


Posted on November 17, 2022
What are the differences between procaspase and caspase?
ApoptosisApoptosis and NecrosisCaspasesCell Viability AssaysCellular ProcessesEnzymes


Posted on November 17, 2022
How do I stain cells with propidium iodide?
Apoptosis and NecrosisBioconjugationCell Cycle AssaysCell Proliferation AssaysCell Viability AssaysCellular ProcessesNucleus


Posted on November 17, 2022
How do I dissolve propidium iodide (PI)?
Apoptosis and NecrosisCell Cycle AssaysCell Structures a nd OrganellesCell Viability AssaysNucleus


Posted on November 7, 2022
How many types of cell death are there?
Apoptosis and NecrosisAutophagyCell Viability AssaysCellular ProcessesCellular Structures and Organellescell death


Posted on November 7, 2022
Why are Annexin V conjugates and 7-AAD used together?
Chemical ReagentsAnnexin-V StainingApoptosis and NecrosisCell Cycle AssaysCell Viability AssaysCellular ProcessesCellular Structures and OrganellesClassic DyesFluoresceinsNucleusannexin V


Posted on November 7, 2022
Where are apoptotic bodies found?
Apoptosis and NecrosisCell Viability AssaysCellular ProcessesCellular Structures and Organellescell death


Posted on November 7, 2022
What do apoptotic bodies contain?
Apoptosis and NecrosisCell Viability AssaysCellular ProcessesCellular Structures and Organellescell death


Posted on November 7, 2022
Which cellular organelles are involved in initiation of the intrinsic pathway of apoptosis?
Apoptosis and NecrosisCell Viability AssaysCellular ProcessesCellular Structures and OrganellesMitochondriamitochondria membrane potential


Posted on November 7, 2022
How do caspases execute apoptosis?
Apoptosis and NecrosisCaspasesCell Viability AssaysCellular ProcessesEnzymescell death


Posted on November 7, 2022
What are apoptotic signals?
Antibodies and ProteomicsApoptosis and NecrosisCell Viability AssaysCellular Processescell death


Posted on November 7, 2022
What is the significance of apoptosis?
Apoptosis and NecrosisCell Viability AssaysCellular Processescell death


Posted on October 26, 2022
Does propidium iodide stain apoptotic cells?
BioconjugationApoptosis and NecrosisCell Cycle AssaysCell Structures and OrganellesCell Viability AssaysNucleus


Posted on October 26, 2022
What does propidium iodide measure?
Antibody and Protein LabelingApoptosis and NecrosisCell Structures and OrganellesCell Viability AssaysNucleus


Posted on October 25, 2022
What are the types of signaling molecules?
BioconjugationApoptosis and NecrosisCell SignalingCellular ProcessesG-Protein-Coupled Receptors (GPCR)Intracellular Ions


Posted on October 14, 2022
What are the most common types of cell death?
Apoptosis and NecrosisCell Viability AssaysCellular ProcessesPhysiological Probes


Posted on August 9, 2022
What are the differences between ADP and ATP?
Cell CytotoxicityPhysiological ProbesApoptosis and Necrosis


Posted on March 8, 2022
What is the composition of a DNA fragment?
Cell Structures and OrganellesCellular ProcessesApoptosis and NecrosisDNA and RNA Quantitation


Posted on February 16, 2022
What is DNA fragmentation?
Apoptosis and NecrosisCellular ProcessesCell Structures and OrganellesNucleusDNA and RNA QuantitationPhysiological Probes


Posted on January 7, 2022
What are organoid cultures?
Cell ViabilityCellular ProcessesApoptosis and NecrosisPhysiological Probes


Posted on December 10, 2021
Can 7-AAD stain live cells?
Cell ViabilityCell CytotoxicityCellular ProcessesApoptosis and Necrosis


Posted on December 9, 2021
Does pH and staining temperature affect Annexin V-Phosphatidylserine binding?
Cell ViabilityCell CytotoxicityCellular ProcessesApoptosis and NecrosisFlow Cytometry Reagents


Posted on December 9, 2021
Why do I need to use Annexin V Binding Buffer?
Cell ViabilityCell CytotoxicityApoptosis and NecrosisFlow Cytometry Reagents


Posted on October 15, 2021
What are inflammasome inhibitors?
EnzymesCell ViabilityApoptosis and NecrosisCaspases


Posted on October 15, 2021
Can ROS trigger inflammasome activation?
Reactive Oxygen Species (ROS)Apoptosis and NecrosisCell ViabilityPhysiological Probes


Posted on October 15, 2021
Are inflammasomes and caspase-1 related?
Apoptosis and NecrosisCaspasesEnzymesCell Viability


Posted on October 13, 2021
How is NLRP3 activation detected?
Cell MetabolismCell Structures a nd OrganellesMembrane Potential and ChannelsApoptosis and Necrosis


Posted on October 13, 2021
How is ASC oligomerization detected?
Apoptosis and NecrosisEnzymesCell ViabilityPhysiological Probes


Posted on October 13, 2021
What are ASC oligomers?
Apoptosis and NecrosisCell ViabilityEnymes


Posted on October 13, 2021
What is the primary function of the NLRP3 inflammasome?
Cell Structures and OrganellesApoptosis and NecrosisCell Viability


Posted on October 11, 2021
What is "non canonical" inflammasome activation?
EnzymesCell ViabilityApoptosis and Necrosis


Posted on October 11, 2021
What is the AIM2 inflammasome?
Antibodies and ProteomicsApoptosis and NecrosisCaspases


Posted on October 11, 2021
How are inflammasomes detected?
Cell ViabilityCell CytotoxicityApoptosis and Necrosis


Posted on October 8, 2021
What are the main functions of inflammasomes?
EnzymesCell ViabilityApoptosis and Necrosis


Posted on October 6, 2021
What are the 3 main components of an inflammasome?
EnzymesCell ViabilityApoptosis and Necrosis


Posted on October 5, 2021
What are inflammasomes?
EnzymesCell ViabilityApoptosis and Necrosis


Posted on September 10, 2021
Can BrdU (Bromodeoxyuridine) be used in vitro?
BioconjugationCell ViabilityCell CytotoxicityApoptosis and Necrosis


Posted on April 19, 2021
What are the main components of homeostasis?
Apoptosis and NecrosisCellular Processes


Posted on April 16, 2021
What is the process of self-destruction of the cell nucleus (often referred to as "programmed cell death") known as?
Apoptosis and NecrosisCellular Processes


Posted on November 25, 2020
What is the definition of apoptosis?
Cell ViabilityCell cytotoxicityCellular processesApoptosis and Necrosis


Posted on October 21, 2020
How fast does apoptosis occur?
Cellular ProcessesApoptosis and NecrosisAnnexin-V Staining


Posted on October 13, 2020
Is apoptosis good or bad?
Cell CytotoxicityApoptosis and NecrosisCell Viability


Posted on May 18, 2020
Why are reactive oxygen species (ROS) bad?
Apoptosis and NecrosisCell SignalingCellular MetabolismCellular ProcessesEnzymesPhysiological ProbesReactive Oxygen Species (ROS)


Posted on May 18, 2020
What are apoptotic markers?
Apoptosis and NecrosisCaspasesCell ViabilityCell Viability AssaysCellular ProcessesCellular Structures and OrganellesTUNELannexin Vmitochondria membrane potential


Posted on May 18, 2020
How do you detect apoptosis?
Chemical ReagentsAnnexin-V StainingApoptosis and NecrosisCell ViabilityCell Viability AssaysCellular ProcessesClassic DyesPhysiological ProbesTUNELannexin V


Posted on May 18, 2020
What is the essential difference between apoptosis and necrosis?
Apoptosis and NecrosisCell ViabilityCell Viability AssaysCellular ProcessClassic DyesPhysiological Probesannexin Vcell death


Posted on May 18, 2020
How do you identify stages of apoptosis?
Chemical ReagentsAnnexin-V StainingApoptosis and NecrosisCellular ProcessesCellular Structures and OrganellesClassic DyesPhysiological ProbesTUNELannexin V


Posted on May 9, 2020
Are Annexin V-FITC+/PI+ cells classified as late-apoptotic cells or necrotic cells?
Chemical ReagentsAV-FITCAnnexin-V StainingApoptosisApoptosis and NecrosisCell ViabilityCell Viability AssaysClassic DyesPropidium iodide (PI)annexin V


Posted on May 9, 2020
Can apoptosis be detected by cell cycle studies?
Chemical ReagentsAnnexin-V StainingApoptosisApoptosis and NecrosisCell ViabilityCell Viability AssaysCellular ProcessesClassic DyesTUNELannexin V


Posted on May 9, 2020
What stain should I use to analyze apoptosis in flow cytometry?
Annexin-V StainingApoptosisApoptosis and NecrosisCell ViabilityCell Viability AssaysClassic DyesFlow Cytometry ReagentsFluoresceinsannexin Vflow cytometry


Posted on May 9, 2020
What are the best methods to identify necrotic cells?
Antibodies and ProteomicsApoptosisApoptosis and NecrosisCell ViabilityCell Viability AssaysCellular ProcessesDehydrogenaseEnzymesFlow Cytometry Reagentsflow cytometry


Posted on May 9, 2020
What does elevated fluorescence of PI alone in Annexin V/PI staining indicate?
Annexin-V StainingApoptosisApoptosis and NecrosisCell ViabilityCell Viability AssaysFlow Cytometry Reagentsannexin V


Posted on April 17, 2020
Why should a cell die or undergo apoptosis?
ApoptosisApoptosis and NecrosisCell ViabilityCellular Processescell deathFlow Cytometry ReagentsPhysiological Probesflow cytometry


Posted on April 17, 2020
What is the purpose of using cold PBS in the washing step of Annexin V staining?
Chemical ReagentsAnnexin-V StainingApoptosisApoptosis and NecrosisCell MetabolismCell ViabilityCellular ProcessesPBSannexin V