Chemical Reagents | AAT Bioquest

Posted on July 25, 2024
How does accumulation of end-products affect enzyme activity?
Cellular ProcessesChemical ReagentsEnzymesPhysiological Probes

Posted on March 13, 2024
Are Lewis acid and Bronsted acid the same?
Chemical ReagentsIntracellular pH

Posted on July 6, 2023
Do enzymes decrease the activation energy of a reaction?
Chemical ReagentsEnzymesPhysiological Probes

Posted on July 6, 2023
How do enzymes achieve specificity?
Chemical ReagentsEnzymesPhysiological Probes

Posted on July 6, 2023
Does the presence of cofactors affect enzyme activity?
Chemical ReagentsEnzymesPhysiological Probes

Posted on June 27, 2023
How does mass spectrometry identify proteins?
Antibodies and ProteomicsAntibody and Protein LabelingChemical ReagentsPhysiological Probes

Posted on June 27, 2023
How do cell viability assay work?
Apoptosis and NecrosisCell SignalingCell Viability AssaysCellular ProcessesChemical ReagentsLive or DeadPropidium iodide (PI)

Posted on June 21, 2023
What are the pros of membrane asymmetry cell viability assays?
Annexin-V StainingApoptosis and NecrosisCell Viability AssaysCellular ProcessesChemical ReagentsClassic Dyes

Posted on June 21, 2023
What are the advantages of using mass spectrometry (MS) to detect phosphorylated Proteins?
Antibodies and ProteomicsAntibody and Protein LabelingChemical ReagentsPhysiological ProbesProtein

Posted on May 9, 2023
What are the relationships between FACS and flow cytometry?
BioconjugationChemical ReagentsFlow Cytometry ReagentsFluorescenceSpectral Flow Cytometry

Posted on May 9, 2023
What are the applications of flow cytometry and FACS?
BioconjugationApoptosisCell Cycle AnalysisCell Proliferation AssaysChemical ReagentsFlow Cytometry ReagentsFluorescenceSpectral Flow Cytometry

Posted on May 2, 2023
What would happen if I forgot to add the sample loading buffer?
Buffer Preparations and RecipesChemical Reagentsbuffer

Posted on May 2, 2023
What are the two ways to label DNA molecules?
BioconjugationCellular Structures and OrganellesChemical ReagentsDNADNA and RNA QuantitationNucleic AcidsNucleusPhysiological Probes

Posted on January 12, 2023
What are the factors that cause fluorescence quenching?
Bioconjugation Förster Resonance Energy Transfer (FRET)Chemical ReagentsFluorescencePhysiological ProbesTide Quencher Dyes

Posted on December 14, 2022
Can atoms be subdivided?
BioconjugationChemical Reagents

Posted on November 14, 2022
What are the applications of GFP?
Antibodies and Proteomics Bioconjugation Chemical ReagentsAntibody and Protein LabelingChemical ReagentsClassic DyesGreen FluorescenceProtein

Posted on November 8, 2022
Are amino acids zwitterionic?
Antibody and Protein LabelingChemical ReagentsDye to Peptide ConjugationNucleusPhysiological Probes

Posted on October 14, 2022
Is coumarin legal?
BioconjugationChemical ReagentsClassic DyesCoumarins

Posted on October 14, 2022
Where does coumarin come from?
BioconjugationChemical ReagentsClassic DyesCoumarins

Posted on October 14, 2022
Is coumarin a natural chemical?
BioconjugationChemical ReagentsClassic DyesCoumarins

Posted on October 14, 2022
Is there a better dye than AMCA?
BioconjugationChemical ReagentsClassic DyesCoumarins

Posted on October 14, 2022
What are coumarin derivatives used for?
BioconjugationChemical ReagentsClassic DyesCoumarins

Posted on October 14, 2022
What is coumarin used for?
BioconjugationChemical ReagentsClassic DyesCoumarins

Posted on October 7, 2022
How do cells recognize signals?
Calcium IndicatorsChemical ReagentsIntracellular IonsPhysiological Probes

Posted on July 21, 2022
What are the applications of phage display technology?
BioconjugationChemical ReagentsAntibodies and ProteomicsAntibody and Protein Labeling

Posted on May 25, 2022
How to choose the right method of crosslinking?
CrosslinkersBioconjugationChemical ReagentsProtein to Protein Conjugation

Posted on May 25, 2022
What is crosslinking?
Protein to Protein ConjugationBioconjugationCrosslinkersChemical Reagents

Posted on May 25, 2022
What is chemical cross-linking?
BioconjugationProtein to Protein ConjugationCrosslinkersChemical Reagents

Posted on May 24, 2022
Is M+ peak the molecular weight?
Chemical ReagentsPhysiological ProbesAntibody and Protein Labeling

Posted on May 24, 2022
Is the M+ peak the molecular ion peak?
Physiological ProbesAntibody and Protein LabelingChemical Reagents

Posted on May 24, 2022
What is a molecular ion peak in mass spectrometry?
Antibody and Protein LabelingChemical ReagentsPhysiological Probes

Posted on May 24, 2022
What causes molecular ion peaks?
Antibody and Protein LabelingChemical ReagentsPhysiological Probes

Posted on May 24, 2022
What are the types of mass spectrometry?
Chemical ReagentsAntibody and Protein LabelingPhysiological Probes

Posted on May 24, 2022
What is the base peak?
Physiological ProbesChemical ReagentsAntibody and Protein Labeling

Posted on May 24, 2022
What does mass spectrometry detect?
Physiological ProbesAntibody and Protein LabelingAntibody and Protein LabelingChemical Reagents

Posted on May 24, 2022
What is the basic principle of mass spectrometry?
Chemical ReagentsAntibody and Protein LabelingPhysiological Probes

Posted on May 24, 2022
How does mass spectrometry work?
Chemical ReagentsAntibody and Protein LabelingPhysiological Probes

Posted on May 24, 2022
How do I find M+ peak?
ToolsAntibody and Protein LabelingChemical Reagents

Posted on May 24, 2022
What kind of substances interfere with mass spectrometry analysis?
Chemical ReagentsToolsPhysiological Probes

Posted on May 24, 2022
What is the difference between a molecular ion peak and a base peak?
Physiological ProbesToolsChemical Reagents

Posted on May 24, 2022
What determines peak height in the mass spectrum?
Chemical ReagentsAntibody and Protein LabelingToolsPhysiological Probes

Posted on May 12, 2022
Do atoms gain or lose mass when they react with each other?
Chemical ReagentsBioconjugationCell Metabolism

Posted on May 12, 2022
Why do some chemical bonds need more energy to break?
Chemical ReagentsClassic DyesCrosslinkersBioconjugation

Posted on May 12, 2022
How do enzymes speed up a chemical reaction?
EnzymesChemical ReagentsPhysiological ProbesCell Metabolism

Posted on April 25, 2022
What are the branches of chemistry and their definition?
Chemical ReagentsClassic Dyes

Posted on April 25, 2022
What are the differences between chemical change and physical change?
Chemical ReagentsClassic Dyes

Posted on April 25, 2022
What are the common grades for chemicals and reagents?
Chemical ReagentsClassic Dyes

Posted on April 22, 2022
What are the differences between reagents and reactants?
Chemical Reagents

Posted on April 22, 2022
What are the primary types of chemical bonds?
Chemical ReagentsClassic Dyes

Posted on April 19, 2022
What are the basic types of chemical reactions?
Chemical ReagentsPhysiological ProbesClassic Dyes

Posted on April 18, 2022
What are the indicators of chemical reactions?
Chemical ReagentsEnzymes

Posted on April 15, 2022
What is chemical bonding?
Chemical Reagents

Posted on April 15, 2022
How does dilution affect molarity?
ToolsChemical Reagents

Posted on April 14, 2022
What are the differences between lysine and arginine?
Cell Structures and OrganellesChemical ReagentsPhysiological ProbesBioconjugation

Posted on April 14, 2022
What are the categories of amino acids?
Cell Structures and OrganellesChemical ReagentsBioconjugationPhysiological ProbesEnzymes

Posted on March 25, 2022
What are the applications of capillary electrophoresis?
BioconjugationChemical ReagentsDyes by Functional GroupAntibody and Protein Labeling

Posted on March 25, 2022
What are the advantages of fragment analysis?
Antibodies and ProteomicsAntibody and Protein LabelingChemical ReagentsDNA and RNA Quantitation

Posted on March 25, 2022
How can I improve PCR efficiency?
BioconjugationChemical ReagentsDNA and RNA Quantitation

Posted on March 24, 2022
What are the steps of Sanger sequencing?
Chemical ReagentsDNA and RNA QuantitationAntibody and Protein LabelingPhysiological Probes

Posted on March 24, 2022
What is fragment analysis?
Chemical ReagentsDyes by Functional GroupPhysiological ProbesDNA and RNA Quantitation

Posted on March 24, 2022
How do I determine the amplification efficiency of my qPCR assay?
Chemical ReagentsDNA and RNA QuantitationAntibody and Protein LabelingBioconjugation

Posted on March 24, 2022
What are the applications of RT-qPCR (Quantitative Reverse Transcription PCR)?
Antibody and Protein LabelingDNA and RNA QuantitationChemical ReagentsDyes by Functional Group

Posted on March 24, 2022
What is RT-qPCR (Quantitative Reverse Transcription PCR)?
DNA and RNA QuantitationChemical ReagentsBioconjugationPhysiological Probes

Posted on March 16, 2022
Why is Protein Purification Important?
Antibodies and ProteomicsAntibody and Protein LabelingChemical Reagents

Posted on March 16, 2022
What are the two common approaches used in column chromatography?
Chemical ReagentsClassic DyesDyes by Functional Groups

Posted on March 16, 2022
What are the common methods for protein purification?
Chemical ReagentsClassic DyesAntibodies and ProteomicsAntibody and Protein Labeling

Posted on March 16, 2022
How Does Protein Purification Work?
Antibodies and ProteomicsAntibody and Protein LabelingChemical ReagentsCrosslinkers

Posted on March 10, 2022
What is DNA profiling?
Antibody and Protein LabelingDNA and RNA QuantitationChemical ReagentsPhysiological Probes

Posted on March 3, 2022
What are the differences between a standard PCR and RT PCR?
Reverse Transcription PCR (RT-PCR)Polymerase Chain Reaction (PCR)Chemical ReagentsClassic DyesDNA and RNA Quantitation

Posted on February 25, 2022
What is RT-PCR?
Chemical ReagentsNucleusDNA and RNA QuantitationPhysiological ProbesCell Structures and Organelles

Posted on January 5, 2022
What are the four stages of mass spectrometry?
Chemical ReagentsAntibody and Protein Labeling

Posted on December 13, 2021
What are coumarin dyes used for?
CoumarinsClassic DyesChemical Reagents

Posted on December 1, 2021
What is the formula of ethidium bromide?
Chemical ReagentsNucleusPhysiological ProbesDNA and RNA Quantitation

Posted on November 30, 2021
Is ethidium bromide used in RT PCR?
NucleusPhysiological ProbesDNA and RNA QuantitationChemical Reagents

Posted on October 1, 2021
Which rhodamines are used in biological research laboratories?
Rhodamines and Rhodamine DerivativesClassic DyesChemical ReagentsDyes by Functional Groups

Posted on October 1, 2021
Is Rhodamine 6G soluble in water?
Rhodamines and Rhodamine DerivativesClassic DyesChemical ReagentsDyes by Functional Groups

Posted on October 1, 2021
What is Rhodamine B used for?
Rhodamines and Rhodamine DerivativesClassic DyesChemical ReagentsDyes by Functional Groups

Posted on October 1, 2021
What is the difference between Rhodamine 123 and Rhodamine B?
Chemical ReagentsClassic DyesDyes by Functional GroupsRhodamines and Rhodamine Derivatives

Posted on October 1, 2021
Is Rhodamine B an amphoteric dye?
Rhodamines and Rhodamine DerivativesClassic DyesChemical ReagentsDyes by Functional Groups

Posted on September 30, 2021
How should rhodamines be disposed of?
Rhodamines and Rhodamine DerivativesChemical ReagentsDyes by Functional Groups

Posted on September 30, 2021
What is the chemical formula of Rhodamine B?
Rhodamines and Rhodamine DerivativesChemical ReagentsDyes by Functional Groups

Posted on September 30, 2021
What happens when rhodamine B is in the presence of acid?
Rhodamines and Rhodamine DerivativesChemical ReagentsDyes by Functional Groups

Posted on September 30, 2021
Is rhodamine hydrophilic or hydrophobic?
Rhodamines and Rhodamine DerivativesChemical ReagentsDyes by Functional GroupsClassic Dyes

Posted on September 30, 2021
Is rhodamine a fluorochrome?
Rhodamines and Rhodamine DerivativesChemical ReagentsDyes by Functional GroupsBioconjugation

Posted on September 30, 2021
What are rhodamine dyes?
Rhodamines and Rhodamine DerivativesChemical ReagentsDyes by Functional Groups

Posted on September 29, 2021
What is the difference between Rhodamine B and Rhodamine 6G?
Rhodamines and Rhodamine DerivativesClassic DyesChemical ReagentsDyes by Functional Group

Posted on September 29, 2021
What wavelength does rhodamine B absorb?
Classic DyesChemical ReagentsDyes by Functional GroupRhodamines and Rhodamine Derivatives

Posted on September 29, 2021
What is the quantum yield of rhodamine B?
Classic DyesChemical ReagentsDyes by Functional Group

Posted on September 29, 2021
What other names is rhodamine B called?
Chemical ReagentsClassic DyesDye by Functional Group

Posted on September 29, 2021
Are rhodamines toxic?
Classic DyesChemical ReagentsDyes by Functional GroupRhodamines and Rhodamine Derivatives

Posted on September 29, 2021
Are rhodamines pH-dependent?
Classic DyesChemical ReagentsDyes by Functional Group

Posted on August 13, 2021
How does indocyanine green (ICG) fluorescence work?
Chemical Reagents

Posted on August 13, 2021
What kind of solvents are used for dissolving indocyanine green (ICG)?
Chemical ReagentsDyes by Functional Group

Posted on August 13, 2021
What is the structure of (ICG)?
BioconjugationChemical ReagentsAntibody and Protein Labeling

Posted on August 13, 2021
Can indocyanine green (ICG) be conjugated to antibodies?
BioconjugationChemical ReagentsAntibody and Protein Labeling

Posted on August 12, 2021
Can indocyanine green (ICG) be used in Western blotting?
BioconjugationChemical Reagents

Posted on August 12, 2021
Is indocyanine green (ICG) used for small animal in vivo imaging?
BioconjugationChemical ReagentsClassic Dyes

Posted on August 12, 2021
What are indocyanine green (ICG) hydrazides used for?
BioconjugationChemical Reagents

Posted on August 12, 2021
Are there any alternatives to indocyanine green (ICG)?
Chemical ReagentsBioconjugation

Posted on August 12, 2021
Does indocyanine green (ICG) have a charge?
Chemical ReagentsClassic Dyes

Posted on August 12, 2021
Does indocyanine green (ICG) contain iodine?
Chemical ReagentsDyes by Functional Group

Posted on August 11, 2021
Is indocyanine green (ICG) FDA approved?
Chemical ReagentsClassic DyesDyes by Functional Group

Posted on August 11, 2021
What is indocyanine green (ICG)?
Classic DyesChemical Reagents

Posted on August 10, 2021
What are indocyanine green (ICG) injections used for?
BioconjugationChemical ReagentsDyes by Functional Group

Posted on August 10, 2021
How is indocyanine green administered?
BioconjugationChemical Reagents

Posted on August 10, 2021
How long does indocyanine green (ICG) stay in tissues after being injected?
Chemical ReagentsDyes by Functional GroupClassic Dyes

Posted on July 27, 2021
Can Cy3 be used in flow cytometry?
Chemical ReagentsCycanineClassic Dyes

Posted on July 27, 2021
Are any of the cyanine dyes infrared?
CyaninesClassic DyesChemical Reagents

Posted on July 27, 2021
Which cyanine dyes can be used for in vivo imaging applications?
CyaninesClassic DyesChemical ReagentsPeptide and Oligonucleotide Labeling

Posted on July 20, 2021
What is the difference between Cy5 and Cy5.5?
Classic DyesCyaninesChemical Reagents

Posted on July 20, 2021
Is glycine a zwitterion?
Chemical Reagents

Posted on July 2, 2021
Are zwitterion molecules stable?
Chemical Reagents

Posted on July 2, 2021
Are all phospholipids zwitterionic?
Chemical Reagents

Posted on July 1, 2021
Can Cy5 be used to label DNA oligos?
Chemical Reagentsptide and Oligonucleotide Labeling

Posted on May 3, 2021
What’s the difference between CRISPR and restriction enzymes?
Chemical Reagents

Posted on April 16, 2021
What is the most important determinant of toxicity?
Chemical Reagents

Posted on April 9, 2021
What happens after the acetylation of lysines?
Chemical ReagentsDNA and RNA

Posted on April 9, 2021
What is TD50?
ToolsChemical Reagents

Posted on April 9, 2021
How do you calculate LD50?
ToolsChemical Reagents

Posted on April 9, 2021
What is the difference between LD50 and TD50?
ToolsChemical Reagents

Posted on April 9, 2021
What are the units of LD50?
ToolsChemical Reagents

Posted on April 9, 2021
What is the difference between fatal and lethal toxicity?
ToolsChemical Reagents

Posted on April 8, 2021
What is the LD50 of cyanide?
Chemical Reagents

Posted on April 8, 2021
What is the LD50 of lead?
Chemical Reagents

Posted on April 8, 2021
What does toxicity mean?
Chemical Reagents

Posted on April 8, 2021
What factors influence toxicity level?
Chemical Reagents

Posted on April 8, 2021
What are the four routes of exposure?
Chemical Reagents

Posted on April 7, 2021
What is Forster radius?
Förster Resonance Energy Transfer (FRET)Chemical Reagents

Posted on March 17, 2021
What kind of detector is used in fluorimetry?
BioconjugationChemical ReagentsiFiFluor Dyes and Kits

Posted on March 17, 2021
How do I calculate FRET efficiency?
Chemical ReagentsPeptide and Oligonucleotide LabelingFörster Resonance Energy Transfer (FRET)

Posted on March 17, 2021
What is FRET efficiency?
Peptide and Oligonucleotide LabelingFörster Resonance Energy Transfer (FRET)Chemical Reagents

Posted on March 17, 2021
What does FRET mean?
Förster Resonance Energy Transfer (FRET)Peptide and Oligonucleotide LabelingChemical ReagentsClassic Dyes

Posted on March 17, 2021
How do you choose FRET pairs?
Chemical ReagentsFörster Resonance Energy Transfer (FRET)Peptide and Oligonucleotide Labeling

Posted on March 16, 2021
What is FRET imaging?
Classic DyesChemical ReagentsFörster Resonance Energy Transfer (FRET)

Posted on March 16, 2021
What is FRET technique?
Classic DyesChemical Reagents

Posted on March 16, 2021
What makes a good fluorophore?
Classic DyesChemical Reagents

Posted on March 16, 2021
Is a fluorophore and a fluorochrome the same thing?
Classic DyesChemical Reagents

Posted on February 5, 2021
What is digoxigenin?
Chemical ReagentsDyes by Functional Group

Posted on January 29, 2021
What are the differences between a fluorophore and a chromophore?
Chemical ReagentsDyes by Functional GroupFörster Resonance Energy Transfer (FRET)

Posted on January 26, 2021
How are oligonucleotides synthesized?
Tide Quencher DyesPeptide and Oligonucleotide LabelingFörster Resonance Energy Transfer (FRET)Chemical Reagents

Posted on January 26, 2021
What are oligonucleotides used for?
Chemical ReagentsPeptide and Oligonucleotide Labeling

Posted on November 30, 2020
How do I calculate PCR efficiency?
DNA and RNAChemical ReagentsClassic Dyes

Posted on November 30, 2020
What is the acceptable PCR primer efficiency value?
DNA and RNAPhysiological ProbesNucleusChemical Reagents

Posted on November 25, 2020
What is stringency in hybridization?
Chemical ReagentsPhysiological ProbesDNA and RNANucleus

Posted on November 25, 2020
How do I evaluate the performance of a real-time PCR reaction?
Classic DyesDNA and RNAChemical Reagents

Posted on November 24, 2020
How can I improve the PCR output of a low concentrated DNA?
Chemical ReagentsDNA and RNAPhysiological Probes

Posted on November 3, 2020
How do I determine cytotoxicity?
Peptide and OligonucleotideChemical ReagentsFörster Resonance Energy Transfer (FRET)

Posted on October 28, 2020
How do restriction enzymes work?
Chemical Reagents

Posted on October 21, 2020
How are oligos made?
Peptide and Oligonucleotide LabelingChemical ReagentsDyes by Functional Group

Posted on April 17, 2020
What is IHC double staining?
Antibodies and ProteomicsAntibody and Protein LabelingImmunohistochemistry (IHC)Chemical ReagentsClassic DyesIHCPhysiological Probes