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AAT Bioquest

Posted on May 2, 2024
What are the different types of blocking buffers?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingBuffer Preparations and RecipesImmunohistochemistry (IHC)


Posted on April 19, 2024
What factors should I consider when choosing a DNA extraction method?
Chemical ReagentsBuffer Preparations and RecipesCellular Structures and OrganellesDNA and RNA QuantitationDNA extractionNucleic AcidsNucleuscell lysis


Posted on April 19, 2024
What is the dialysis method in protein isolation?
Chemical ReagentsBuffer Preparations and Recipesaffinity purificationcell lysisdetergent


Posted on April 19, 2024
What is the purpose of dialysis in protein isolation?
Chemical ReagentsBuffer Preparations and Recipesaffinity purificationcell lysisdetergent


Posted on April 19, 2024
What factors affect the rate of dialysis?
Chemical ReagentsBuffer Preparations and Recipesaffinity purificationcell lysisdetergent


Posted on April 19, 2024
What are the principles of dialysis?
Chemical ReagentsBuffer Preparations and Recipesaffinity purificationcell lysisdetergent


Posted on April 19, 2024
What is a typical dialysis procedure
Chemical ReagentsBuffer Preparations and Recipesaffinity purificationcell lysisdetergent


Posted on April 19, 2024
What is protein desalting?
Chemical ReagentsBuffer Preparations and Recipesaffinity purificationcell lysisdetergent


Posted on April 19, 2024
What are the methods used for protein desalting?
Chemical ReagentsBuffer Preparations and Recipesaffinity purificationcell lysisdetergent


Posted on April 19, 2024
How do I optimize protein desalting?
Chemical ReagentsBuffer Preparations and Recipesaffinity purificationcell lysisdetergent


Posted on April 19, 2024
What are the differences between desalting and buffer exchange?
Chemical ReagentsBuffer Preparations and Recipesaffinity purificationcell lysisdetergent


Posted on April 19, 2024
What is diafiltration?
Chemical ReagentsBuffer Preparations and RecipesProtein Purificationaffinity purificationcell lysisdetergent


Posted on April 19, 2024
What are the applications of diafiltration?
Chemical ReagentsBuffer Preparations and RecipesProtein Purificationaffinity purificationcell lysisdetergent


Posted on April 19, 2024
What is the process of diafiltration?
Chemical ReagentsBuffer Preparations and RecipesProtein PurificationSerial Dilutionaffinity purificationcell lysisdetergent


Posted on April 19, 2024
What are the benefits of diafiltration?
Chemical ReagentsBuffer Preparations and RecipesProtein PurificationSerial Dilutionaffinity purificationcell lysisdetergent


Posted on April 10, 2024
How to eliminate non-specific binding?
Antibodies and Proteomics Bioconjugation Chemical ReagentsAntibody and Protein LabelingBuffer Preparations and RecipesPhysiological Probes


Posted on April 10, 2024
How does electrophoretic transfer work?
Chemical ReagentsBuffer Preparations and Recipes


Posted on April 10, 2024
What is electrophoretic transfer?
Chemical ReagentsBuffer Preparations and Recipes


Posted on April 10, 2024
What are the disadvantages of using dried milk as a blocking buffer?
Antibodies and Proteomics Bioconjugation Chemical ReagentsAntibody and Protein LabelingBuffer Preparations and Recipes


Posted on April 10, 2024
What are the advantages of using dried milk as a blocking buffer?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingBSABuffer Preparations and Recipes


Posted on April 10, 2024
What are the components of an extraction buffer?
Chemical ReagentsBuffer Preparations and RecipesDNA extractionExtractioncell lysisdetergent


Posted on April 10, 2024
What is the significance of an extraction buffer?
Chemical ReagentsBuffer Preparations and RecipesDNA extractionExtractioncell lysisdetergent


Posted on December 22, 2023
How do I lyse cells using physical methods?
Buffer Preparations and RecipesDialysisExtractioncell lysis


Posted on December 22, 2023
Why is lysis important in DNA extraction?
Buffer Preparations and RecipesDialysisExtractioncell lysis


Posted on December 22, 2023
How do I lyse cells using chemical methods?
Buffer Preparations and RecipesDialysisExtractioncell lysis


Posted on December 22, 2023
What is a phosphate buffer solution?
Buffer Preparations and RecipesPBS


Posted on December 22, 2023
What are the advantages of phosphate buffer?
Buffer Preparations and RecipesPBS


Posted on December 22, 2023
What are the disadvantages of phosphate buffer?
Buffer Preparations and RecipesPBS


Posted on December 22, 2023
What are the three major buffer systems?
Buffer Preparations and RecipesPBS


Posted on December 22, 2023
What is a buffer?
Buffer Preparations and RecipesIntracellular pH


Posted on December 22, 2023
How do buffers work?
Buffer Preparations and Recipes


Posted on December 22, 2023
What makes a good buffer?
Buffer Preparations and Recipes


Posted on December 22, 2023
What is a buffer composed of?
Buffer Preparations and Recipes


Posted on December 22, 2023
How do I choose the right biological buffer?
Buffer Preparations and Recipes


Posted on December 22, 2023
At what pH does a buffer solution exhibit maximum buffer capacity?
Buffer Preparations and RecipesIntracellular pH


Posted on December 22, 2023
When do buffers work the best?
Buffer Preparations and RecipesIntracellular pH


Posted on December 22, 2023
What is buffer capacity?
Buffer Preparations and RecipesIntracellular pH


Posted on December 22, 2023
When is buffer capacity at maximum?
Buffer Preparations and RecipesIntracellular pH


Posted on December 22, 2023
How do I calculate buffer capacity?
Buffer Preparations and RecipesIntracellular pH


Posted on May 2, 2023
What would happen if I forgot to add the sample loading buffer?
Buffer Preparations and RecipesChemical Reagentsbuffer


Posted on May 2, 2023
What are the components in the sample loading buffer?
Chemical ReagentsBuffer Preparations and Recipesbuffer


Posted on April 26, 2023
What materials are needed to make a phosphate buffer?
Chemical ReagentsBuffer Preparations and Recipesbuffer


Posted on April 26, 2023
Why is a phosphate buffer solution especially useful for biological applications?
Chemical ReagentsBuffer Preparations and Recipesbuffer


Posted on April 26, 2023
What criteria should I use to determine the type of lysis buffer required for an experiment?
Chemical ReagentsBuffer Preparations and Recipesbuffer


Posted on April 26, 2023
What are the common lysis buffers for general protein extraction?
Chemical ReagentsBuffer Preparations and Recipesbuffer


Posted on April 26, 2023
How do I determine the concentration of an unknown acid or base solution?
Chemical ReagentsBuffer Preparations and Recipesbuffer


Posted on April 26, 2023
What are the most common buffers used for agarose gel electrophoresis?
Chemical ReagentsAgarose gel electrophoresisBuffer Preparations and RecipesGel Electrophoresisbuffer


Posted on April 26, 2023
When should I use a TBE buffer for gel electrophoresis?
Chemical ReagentsAgarose gel electrophoresisBuffer Preparations and RecipesGel Electrophoresisbuffer


Posted on April 26, 2023
When should I use a TAE buffer for gel electrophoresis?
Chemical ReagentsAgarose gel electrophoresisBuffer Preparations and RecipesGel Electrophoresisbuffer


Posted on April 14, 2023
Can homogeneous mixtures be separated?
Chemical ReagentsBuffer Preparations and RecipesStock SolutionWorking Solution


Posted on April 14, 2023
How do I separate mixtures and solutions?
Chemical ReagentsBuffer Preparations and RecipesStock SolutionWorking Solution


Posted on April 14, 2023
How do I prepare a solution of a specific concentration?
Buffer Preparations and RecipesStock SolutionWorking Solutionbuffer


Posted on March 17, 2023
What are the different types of mixture based on the size of the particles involved?
Chemical ReagentsBuffer Preparations and RecipesStock Solutionbuffer


Posted on December 8, 2022
What are the factors that will affect the rate and effectiveness of tissue fixation?
Chemical ReagentsBuffer Preparations and RecipesFixationfixed cells


Posted on December 8, 2022
What are the types of fixatives?
Chemical ReagentsBuffer Preparations and RecipesFixationfixed cells


Posted on December 8, 2022
What are the general types of fixation?
Chemical ReagentsBuffer Preparations and RecipesFixationfixed cells


Posted on December 8, 2022
What are the commonly used chemical fixatives for microscopy?
Chemical ReagentsBuffer Preparations and RecipesFixationfixed cells


Posted on December 8, 2022
What are the functions of fixative?
Chemical ReagentsBuffer Preparations and RecipesFixationfixed cells


Posted on December 8, 2022
What are the properties of an ideal fixative?
Chemical ReagentsBuffer Preparations and RecipesFixationfixed cells


Posted on December 8, 2022
What are the differences between formalin and formaldehyde?
Chemical ReagentsBuffer Preparations and RecipesFixationfixed cells


Posted on December 8, 2022
Is formaldehyde a crosslinking agent?
Chemical ReagentsBuffer Preparations and RecipesFixationfixed cells


Posted on December 8, 2022
Why do tissues require fixation?
Chemical ReagentsBuffer Preparations and RecipesFixationfixed cells


Posted on December 8, 2022
What is the purpose of cell fixation?
Chemical ReagentsBuffer Preparations and RecipesFixationfixed cells


Posted on November 8, 2022
What kind of washing buffer should I use for immunoprecipitation protocols?
ToolsBioconjugationBuffer Preparations and Recipes


Posted on October 4, 2022
What is the main function of the TE buffer?
Buffer Preparations and RecipesDNA and RNA QuantitationPhysiological ProbesTools


Posted on October 4, 2022
Why is EDTA used in the TE buffer?
Buffer Preparations and RecipesDNA and RNA QuantitationPhysiological ProbesTools


Posted on October 4, 2022
Why is Tris used in the TE buffer?
ToolsBuffer Preparations and RecipesDNA and RNA QuantitationPhysiological Probes


Posted on October 3, 2022
What is the composition of TE buffer?
ToolsBuffer Preparations and RecipesPhysiological Probes


Posted on October 3, 2022
What is the function of TE buffer in DNA extraction?
Buffer Preparations and RecipesPhysiological Probes


Posted on September 29, 2022
What is TE (Tris-EDTA) buffer?
Buffer Preparations and RecipesPhysiological Probes Tools


Posted on February 10, 2022
What are the commonly used discontinuous gel buffer systems?
Gel ElectrophoresisPhysiological ProbesBioconjugationBuffer Preparations and Recipes


Posted on June 1, 2020
What is the difference between PAGE and SDS-PAGE?
Buffer Preparations and RecipesGel ElectrophoresisGel Electroporesisbufferdetergent


Posted on May 9, 2020
What is the best method for permeabilizing cells?
Antibodies and Proteomics Chemical ReagentsBuffer Preparations and RecipesCellular Structures and OrganellesPBSPermeabilizationbuffercell lysisdetergent


Posted on May 9, 2020
For primer dilution in PCR, should I use water or TE buffer?
Chemical ReagentsBuffer Preparations and RecipesPCRPolymerase Chain Reaction (PCR)buffer


Posted on April 24, 2020
How can you detect endogenous expression of early endosome and lysosome in immunofluorescence?
Chemical ReagentsBSABuffer Preparations and RecipesImmunohistochemistry (IHC)LysosomesPBSbufferimmunofluorescence


Posted on April 24, 2020
What is the difference between staining buffer and FACS buffer?
Chemical ReagentsBSABuffer Preparations and RecipesEDTAImmunohistochemistry (IHC)PBSbuffer


Posted on April 24, 2020
How do you dilute primary antibodies from 1:200 to 1:1000?
Chemical ReagentsBuffer Preparations and RecipesCalculating ConcentrationIHCImmunohistochemistry (IHC)Serial Dilutionbuffer


Posted on April 24, 2020
What is the difference between bicarbonate and PBS as a coating buffer in ELISA assay?
Chemical Reagents Enzyme-Linked Immunosorbent Assay (ELISA)Buffer Preparations and RecipesELISAPBSbuffer


Posted on April 17, 2020
What is the benefit of using TN buffer (10 mM Tris, 400 mM Nacl, pH 7.5) in the RNA extraction?
Antibodies and Proteomics Chemical ReagentsBuffer Preparations and RecipesDNA and RNAExtractionRNAbuffer


Posted on April 17, 2020
What is the function of adding BSA as buffer in Biotin-Streptavidin system?
Antibodies and Proteomics Bioconjugation Chemical ReagentsAntibody and Protein LabelingBSABiotin and StreptavidinBuffer Preparations and Recipesbovine serum albuminbuffer


Posted on April 17, 2020
How does PBS clean cells?
Chemical ReagentsBuffer Preparations and RecipesIHCImmunohistochemistry (IHC)PBSbuffer


Posted on April 17, 2020
What is the actual mechanism involved in blocking a nitrocelluolse membrane ?
Antibodies and Proteomics Bioconjugation Chemical ReagentsBSABuffer Preparations and Recipesbovine serum albuminbuffer


Posted on April 17, 2020
Does PBS buffer have any advantage over SSC buffer?
Bioconjugation Chemical ReagentsBuffer Preparations and RecipesDNA and RNAFISHPhysiological Probesbufferfluorescence microscopein situ hybridization


Posted on April 17, 2020
Best hybridization buffer for DNA Hybridization?
Buffer Preparations and Recipesbuffer


Posted on April 17, 2020
Does PBS buffer have any effect on cell adhesion?
Buffer Preparations and RecipesPBSadherent cellsbuffer Chemical Reagents


Posted on April 17, 2020
Does adding triton with the primary antibody hinder antigen detection in immunohistochemistry?
Antibodies and ProteomicsAntibody and Protein LabelingImmunohistochemistry (IHC)Primary Antibodies Chemical ReagentsBuffer Preparations and RecipesIHCbufferdetergent


Posted on April 17, 2020
What is the best blocking buffer for immunofluorescence ?
Antibody and Protein LabelingBSABuffer Preparations and RecipesImmunohistochemistry (IHC)PBSbufferimmunofluorescence


Posted on April 17, 2020
Why do we use Citrate buffer in Antigen retrieval step during IHC?
Antibodies and ProteomicsAntibody and Protein LabelingImmunohistochemistry (IHC)buffer Chemical ReagentsBuffer Preparations and Recipes


Posted on April 17, 2020
When should DTT be added in tissue lysis?
Antibodies and Proteomics Chemical ReagentsBuffer Preparations and RecipesPhysiological Probesbuffer


Posted on April 17, 2020
Should I use cold or warm PBS washes when performing protein extraction?
Buffer Preparations and RecipesPBSbuffer Antibodies and ProteomicsEnzymesExtractionProtein


Posted on April 17, 2020
What is the role of Tween-20 in antigen retrieval buffer?
Immunohistochemistry (IHC)detergent Antibodies and Proteomics Chemical ReagentsBuffer Preparations and RecipesIHCbuffer


Posted on April 17, 2020
What is the advantage or dissadvantage of having Tween 20 in flow cytometry wash and staining buffers?
Antibodies and Proteomics Chemical ReagentsBuffer Preparations and RecipesFlow Cytometry ReagentsSpectral Flow Cytometrybufferdetergent


Posted on April 17, 2020
What's the role of BSA/Milk as blocking agents??
Antibodies and Proteomics Bioconjugation Enzyme-Linked Immunosorbent Assay (ELISA)Antibody and Protein LabelingBSABuffer Preparations and RecipesImmunohistochemistry (IHC)bovine serum albuminbuffer


Posted on April 17, 2020
Is agitation necessary during IHC antibody incubation?
Antibodies and ProteomicsBuffer Preparations and RecipesIHCImmunohistochemistry (IHC)Permeabilizationbufferdetergent


Posted on April 17, 2020
What is the purpose of a buffered solution used for RNase treatment?
Chemical ReagentsBuffer Preparations and RecipesDNA PurityDNA and RNAEnzymesNucleic Acidsbuffer


Posted on April 17, 2020
What % of BSA to use for blocking step?
Antibodies and Proteomics Chemical ReagentsBSABuffer Preparations and RecipesPhysiological Probesbovine serum albuminbuffer