Cell Viability | AAT Bioquest

Posted on July 17, 2024
What are the applications of viability PCR (vPCr)?
Chemical ReagentsCell ViabilityCellular Structures and OrganellesPhysiological ProbesPlasma MembranePolymerase Chain Reaction (PCR)

Posted on December 15, 2023
What is the purpose of cryopreserving cultured cells?
Chemical ReagentsCell CultureCell Proliferation AssaysCell ViabilityCell countingadherent cellscryopreservation (frozen) sections

Posted on June 15, 2023
How do quiescent cells maintain their viability?
Cell Cycle AnalysisCell Cycle AssaysCell Cycle and ProliferationCell ViabilityCellular Processes

Posted on May 24, 2023
What effect will the cell sorting process have on my cells' viability?
Chemical ReagentsCell ViabilityFlow Cytometry ReagentsSpectral Flow Cytometryflow cytometerflow cytometryFluorescence Activated Cell Sorting (FACS)Cell Sorting

Posted on May 3, 2023
What is the significance of cell signaling?
Cell Cycle AnalysisCell Cycle and ProliferationCell MetabolismCell SignalingCell ViabilityCellular ProcessesG-Protein-Coupled Receptors (GPCR)Membrane Potential and Channels

Posted on May 3, 2023
What are the functions of cell signaling?
Cell Cycle AnalysisCell Cycle and ProliferationCell CytotoxicityCell MetabolismCell SignalingCell ViabilityCellular ProcessesG-Protein-Coupled Receptors (GPCR)Membrane Potential and Channels

Posted on May 3, 2023
How do cells communicate?
Apoptosis and NecrosisCell Cycle AnalysisCell Cycle and ProliferationCell CytotoxicityCell MetabolismCell SignalingCell ViabilityCellular ProcessesG-Protein-Coupled Receptors (GPCR)Membrane Potential and Channels

Posted on November 16, 2022
How does trypan blue distinguish living and dead cells?
BioconjugationCell Proliferation AssaysCell ViabilityCell Viability AssaysCellular Processes

Posted on June 10, 2022
What is the cell wall made of?
Cell Structures and OrganellesCellular ProcessesCell ViabilityBioconjugation

Posted on June 9, 2022
Do fungi have cell walls?
Cell ViabilityCell Structures and OrganellesNucleusEnzymesBioconjugation

Posted on June 9, 2022
Where is the cell wall located?
Cell Structures and OrganellesBioconjugationCell Viability

Posted on June 9, 2022
What are the functions of the cell wall?
Cell Structures and OrganellesCell MetabolismCellular ProcessesPhysiological ProbesCell Viability

Posted on June 6, 2022
What are the examples of eukaryotic cells?
BioconjugationNucleusCellular ProcessesCell ViabilityDNA and RNA QuantitationPhysiological Probes

Posted on June 1, 2022
How many chromosomes do people have?
Physiological ProbesCell ViabilityDNA and RNA QuantitationCell Structures and Organelles

Posted on May 24, 2022
How does 7-AAD bind to DNA?
Flow Cytometry ReagentsCell ViabilityCell Cycle AssaysCell Cytotoxicity

Posted on May 24, 2022
Is 7-AAD cell-permeable?
Cell Cycle AssaysCell CytotoxicityCellular ProcessesCell ViabilityFlow Cytometry Reagents

Posted on May 24, 2022
Can 7-AAD be fixed?
Cell Cycle AssaysCell CytotoxicityCellular ProcessesCell ViabilityFlow Cytometry Reagents

Posted on May 19, 2022
How does a prokaryotic cell divide?
Physiological ProbesCellular ProcessesCell ViabilityCell Cycle and ProliferationCell Metabolism

Posted on May 6, 2022
What type of transport moves material against the concentration gradient with the use of energy?
Physiological ProbesBioconjugationCell ViabilityCellular ProcessesEnzymes

Posted on April 29, 2022
What enzyme breaks hydrogen bonds in DNA replication?
Cellular ProcessesCell CytotoxicityCell ViabilityPhysiological Probes

Posted on April 28, 2022
What are the primary types of cells?
Cell Structures and OrganellesCell ViabilityPhysiological ProbesBioconjugationCell Viability

Posted on January 7, 2022
What are cellular spheroids?
Cellular ProcessesCell ViabilityPhysiological Probes

Posted on January 7, 2022
What is 3D tissue?
Cellular ProcessesCell ViabilityPhysiological Probes

Posted on January 7, 2022
What is a Histotypic culture?
Cell ViabilityCellular ProcessesPhysiological Probes

Posted on January 7, 2022
What is a 3D scaffold?
Cellular ProcessesCell ViabilityPhysiological Probes

Posted on January 7, 2022
Can tissues be 3D printed?
Cellular ProcessesCell ViabilityPhysiological Probes

Posted on January 7, 2022
What are 2D cell cultures used for?
Cellular ProcessesCell ViabilityPhysiological Probes

Posted on January 7, 2022
What is a 3D spheroid?
Cellular ProcessesCell ViabilityPhysiological Probes

Posted on January 7, 2022
What are organoid cultures?
Cell ViabilityCellular ProcessesApoptosis and NecrosisPhysiological Probes

Posted on January 7, 2022
How are 3D cultures made?
Physiological ProbesCellular ProcessesCell Viability

Posted on January 7, 2022
What is 4D cell culture?
Physiological ProbesCell Viability

Posted on January 7, 2022
Is 3D cell culture more expensive than 2D culture?
Physiological ProbesCell Viability

Posted on January 7, 2022
What are the advantages and limitations of 2D cell culture?
Cell ViabilityPhysiological Probes

Posted on January 7, 2022
When did 3D cell culture start being used?
Physiological ProbesEnzymesCell Viability

Posted on December 21, 2021
What is 7-AAD used for?
Cell ViabilityCell Cycle AssaysCellular ProcessesFlow Cytometry Reagents

Posted on December 21, 2021
What is 7-AAD?
DNA and RNA QuantitationCell ViabilityCell Cycle AssaysCellular ProcessesFlow Cytometry Reagents

Posted on December 20, 2021
What does 7-AAD bind to?
Cell ViabilityCell Cycle AssaysCell CytotoxicityPhysiological Probes

Posted on December 17, 2021
Is 7-AAD toxic to cells?
Cell ViabilityCell Cycle AssaysCellular ProcessesCell Cytotoxicity

Posted on December 10, 2021
Can 7-AAD stain live cells?
Cell ViabilityCell CytotoxicityCellular ProcessesApoptosis and Necrosis

Posted on December 10, 2021
Does 7-AAD stain necrotic cells?
Cell ViabilityCell Cycle AssaysCell CytotoxicityCellular ProcessesFlow Cytometry Reagents

Posted on December 9, 2021
Does pH and staining temperature affect Annexin V-Phosphatidylserine binding?
Cell ViabilityCell CytotoxicityCellular ProcessesApoptosis and NecrosisFlow Cytometry Reagents

Posted on December 9, 2021
Why do I need to use Annexin V Binding Buffer?
Cell ViabilityCell CytotoxicityApoptosis and NecrosisFlow Cytometry Reagents

Posted on October 15, 2021
What are inflammasome inhibitors?
EnzymesCell ViabilityApoptosis and NecrosisCaspases

Posted on October 15, 2021
Can ROS trigger inflammasome activation?
Reactive Oxygen Species (ROS)Apoptosis and NecrosisCell ViabilityPhysiological Probes

Posted on October 15, 2021
Are inflammasomes and caspase-1 related?
Apoptosis and NecrosisCaspasesEnzymesCell Viability

Posted on October 13, 2021
How is ASC oligomerization detected?
Apoptosis and NecrosisEnzymesCell ViabilityPhysiological Probes

Posted on October 13, 2021
What are ASC oligomers?
Apoptosis and NecrosisCell ViabilityEnymes

Posted on October 13, 2021
How does ATP activate the inflammasome?
Physiological ProbesCell MetabolismCell Cycle AssaysCell Viability

Posted on October 13, 2021
What is the primary function of the NLRP3 inflammasome?
Cell Structures and OrganellesApoptosis and NecrosisCell Viability

Posted on October 12, 2021
Is AIM2 an NLR protein?
Antibodies and ProteomicsCell Viability

Posted on October 11, 2021
What is "non canonical" inflammasome activation?
EnzymesCell ViabilityApoptosis and Necrosis

Posted on October 11, 2021
How are inflammasomes detected?
Cell ViabilityCell CytotoxicityApoptosis and Necrosis

Posted on October 8, 2021
What are the main functions of inflammasomes?
EnzymesCell ViabilityApoptosis and Necrosis

Posted on October 6, 2021
What are the 3 main components of an inflammasome?
EnzymesCell ViabilityApoptosis and Necrosis

Posted on October 6, 2021
What causes inflammasome activation?
Physiological ProbesCell ViabilityCellular ProcessesCell Metabolism

Posted on October 5, 2021
Where are inflammasomes found?
EnzymesCell ViabilityCellular Processes

Posted on October 5, 2021
What are inflammasomes?
EnzymesCell ViabilityApoptosis and Necrosis

Posted on September 29, 2021
Can rhodamines be used to investigate ROS?
Cell ViabilityCellular ProcessesReactive Oxygen Species (ROS)

Posted on September 10, 2021
Is BrdU (Bromodeoxyuridine) the only thymidine analog?
Cell Proliferation AssaysBioconjugationCell CytotoxicityCell Viability

Posted on September 10, 2021
Is BrdU (Bromodeoxyuridine) staining performed in vivo?
Cell Proliferation AssaysBioconjugationCell ViabilityCell Cytotoxicity

Posted on September 10, 2021
Can BrdU (Bromodeoxyuridine) be used in vitro?
BioconjugationCell ViabilityCell CytotoxicityApoptosis and Necrosis

Posted on September 10, 2021
How long can BrdU-labeled cells be detected?
Cell ViabilityCell CytotoxicityCellular Processes

Posted on September 10, 2021
How does BrdU (Bromodeoxyuridine) cause mutation?
Cell ViabilityBioconjugationCell Cytotoxicity

Posted on September 9, 2021
Does BrdU (Bromodeoxyuridine) affect the cell cycle?
Cell ViabilityCell CytotoxicityCellular Porcesses

Posted on September 9, 2021
Does BrdU (Bromodeoxyuridine) trigger cell death?
Cell ViabilityCell cytotoxicityCellular ProcessesPhysiological Probes

Posted on September 9, 2021
What are terminal transferases?
BioconjugationCell ViabilityTUNEL

Posted on September 3, 2021
Is BrdU (Bromodeoxyuridine) myelotoxic?
Cell ViabilityCell CytotoxicityCellular Processes

Posted on September 3, 2021
How should BrdU (Bromodeoxyuridine) be disposed of?
Cell CytotoxicityCell ViabilityCellular Processes

Posted on September 3, 2021
Are there any alternatives to BrdU (Bromodeoxyuridine)?
Cell CytotoxicityCell ViabilityPhysiological Probes

Posted on September 3, 2021
Is BrdU (Bromodeoxyuridine) toxic?
Cell ViabilityCell CytotoxicityCellular Processes

Posted on September 3, 2021
What is BrdU (Bromodeoxyuridine) used for?
Cell ViabilityCell CytotoxicityCellular Processes

Posted on September 3, 2021
Is BrdU (Bromodeoxyuridine) light sensitive?
Cell ViabilityCell CytotoxicityCellular Processes

Posted on September 3, 2021
Is BrdU (Bromodeoxyuridine) a stain?
Cell ViabilityCell CytotoxicityCellular Processes

Posted on September 3, 2021
What kind of cells does BrdU (Bromodeoxyuridine) stain?
Cell ViabilityCell CytotoxicityCellular Processes

Posted on September 3, 2021
Is BrdU (Bromodeoxyuridine) a teratogen?
Cell ViabilityCell CytotoxicityCellular Processes

Posted on September 3, 2021
Is BrdU (Bromodeoxyuridine) a biohazard?
Cell ViabilityCell CytotoxicityCellular Processes

Posted on September 3, 2021
Is BrdU (Bromodeoxyuridine) radioactive?
Cell ViabilityCell CytotoxicityCellular Processes

Posted on September 3, 2021
Is BrdU (Bromodeoxyuridine) a carcinogen?
Cell ViabilityCell CytotoxicityCellular Processes

Posted on September 3, 2021
What is BrdU (Bromodeoxyuridine)?
Cell ViabilityCell CytotoxicityCellular Processes

Posted on August 30, 2021
Is Calcein, AM toxic to cells?
Calcein AMCell Membrane IntegrityCell Viabilitylive cell tracking

Posted on April 19, 2021
What are the four primary types of tissues in the human body?
Cell Structures and OrganellesCell Viability

Posted on February 10, 2021
Is cell cycle duration the same for all cells?
Cell Cycle and ProliferationCell Viability

Posted on January 15, 2021
In normal mammalian cells, which take longer: the processes that lead to chromosome duplication or those that lead to cell division?
Cell Viability

Posted on January 15, 2021
In cells that undergo very rapid cell division, which phases are decreased or eliminated?
Cell ViabilityCell Cytotoxicity

Posted on January 15, 2021
What are the two major phases of the cell cycle and during which does cell copying take place? During which does cleavage take place?
Cell ViabilityCellular ProcessesCell Cycle and Proliferation

Posted on January 15, 2021
If a cell that is in G1 is not biologically ready to continue on to S phase, what does it do?
Cell ViabilityCellular ProcessesCell Signaling

Posted on January 15, 2021
During which phase of interphase does cell growth occur?
Cell ViabilityCell Cycle and ProliferationCellular Processes

Posted on December 18, 2020
What does attachment efficiency mean?
Cell Cycle and ProliferationPhysiological ProbesCell Viability

Posted on December 18, 2020
What is cell culture synchronization?
Cell ViabilityCellular ProcessesCell Cycle and Proliferation

Posted on December 17, 2020
What are adherent cells?
Cell ViabilityCellular Processes

Posted on December 16, 2020
What types of disaggregation techniques are used to make primary cell cultures?
Cell ViabilityCellular ProcessesCell Cytotoxicity

Posted on December 16, 2020
What is the difference between a cell line and a cell strain?
Cell ViabilityCell CytotoxicityCellular Process

Posted on December 16, 2020
What are the differences between primary and secondary cell cultures?
Cell ViabilityCell Cycle and ProliferationCellular Processes

Posted on December 16, 2020
What is the difference between continuous culture and batch culture techniques?
Cell Cycle and ProliferationCell ViabilityCellular Processes

Posted on December 15, 2020
What does passaging mean in cell culture?
Cellular ProcessesCell ViabilityCell Cycle and Proliferation

Posted on December 15, 2020
How do I make my cell culture immortal?
Cell Cycle and ProliferationPhysiological ProbesCell Viability

Posted on December 7, 2020
What are the types of tissue culture?
Cell Cycle and ProliferationCell Viability

Posted on December 4, 2020
What media should I use for my tissue culture?
Cell ViabilityCell Cycle and Proliferation

Posted on December 4, 2020
What are some examples of tissue culture?
Cell Cycle and ProliferationMembrane Potential and ChannelsCell Viability

Posted on November 25, 2020
How do I determine cell viability?
Cell ViabilityCell CytotoxicityCellular ProcessesEnzymes

Posted on November 25, 2020
What is the definition of apoptosis?
Cell ViabilityCell cytotoxicityCellular processesApoptosis and Necrosis

Posted on November 24, 2020
What is the difference between cell viability and cell proliferation?
Cell ViabilityCell CytotoxicityCellular Processes

Posted on October 19, 2020
What is the difference between ribosome and lysosome?
Cell Structures and OrganellesCell ViabilityLysosomes

Posted on October 13, 2020
Is apoptosis good or bad?
Cell CytotoxicityApoptosis and NecrosisCell Viability

Posted on July 22, 2020
What is calcein AM?
Chemical ReagentsCalcein AMCell ViabilityCell Viability AssaysCellular ProcessesFluoresceinsGreen FluorescencePhysiological Probes

Posted on July 22, 2020
What does calcein AM stain?
Chemical ReagentsCalcein AMCell ViabilityCell Viability AssaysCellular ProcessesFluoresceinsGreen FluorescencePhysiological Probes

Posted on July 22, 2020
How do you dissolve calcein and calcein AM?
Calcein AMCell ViabilityCell Viability AssaysCellular ProcessesFluoresceinsGreen FluorescencePhysiological Probes

Posted on July 22, 2020
How does calcein AM work?
Chemical ReagentsCalcein AMCell ViabilityCell Viability AssaysCellular ProcessesFluoresceinsGreen FluorescencePhysiological Probes

Posted on July 22, 2020
How does propidium iodide staining work?
Cell Cycle AnalysisCell Cycle AssaysCell ViabilityCell Viability AssaysCellular ProcessesDNAPropidium iodide (PI)

Posted on July 22, 2020
How does DAPI work?
Antibodies and Proteomics Bioconjugation Chemical ReagentsCell ViabilityCell Viability AssaysCellular ProcessesDAPI

Posted on July 22, 2020
How does trypan blue work?
Chemical ReagentsCell ViabilityCell Viability AssaysCellular ProcessesPhysiological Probes

Posted on May 18, 2020
Why does trypan blue quench FITC or GFP in flow cytometry?
Chemical ReagentsCell ViabilityCell Viability AssaysCellular ProcessesFlow Cytometry ReagentsSpectral Flow Cytometryflow cytometry

Posted on May 18, 2020
What is trypan blue (TB) assay?
Chemical ReagentsCell ViabilityCell Viability AssaysImmunohistochemistry (IHC)Live or Deadcell-impermeable

Posted on May 18, 2020
Are there upgraded trypan blue derivatives for cell viability testing?
Chemical ReagentsCell ViabilityCell Viability AssaysCellular ProcessesClassic DyesFluoresceinsFluorescencePhysiological Probes

Posted on May 18, 2020
What is cell viability assay?
Antibodies and Proteomics Chemical ReagentsCell ViabilityCell Viability AssaysCellular ProcessesImmunohistochemistry (IHC)

Posted on May 18, 2020
What dyes are used in flow cytometry for cell viability testing?
Cell ViabilityCell Viability AssaysCell-Impermeable DNA dyesFlow Cytometry ReagentsFluorescenceSpectral Flow Cytometrycell-impermeableflow cytometry

Posted on May 18, 2020
What is cell sorting in flow cytometry?
Antibodies and ProteomicsCell ViabilityCell Viability AssaysCellular ProcessesFlow Cytometry ReagentsSpectral Flow Cytometryflow cytometry

Posted on May 18, 2020
What are apoptotic markers?
Apoptosis and NecrosisCaspasesCell ViabilityCell Viability AssaysCellular ProcessesCellular Structures and OrganellesTUNELannexin Vmitochondria membrane potential

Posted on May 18, 2020
How do you detect apoptosis?
Chemical ReagentsAnnexin-V StainingApoptosis and NecrosisCell ViabilityCell Viability AssaysCellular ProcessesClassic DyesPhysiological ProbesTUNELannexin V

Posted on May 18, 2020
What is the essential difference between apoptosis and necrosis?
Apoptosis and NecrosisCell ViabilityCell Viability AssaysCellular ProcessClassic DyesPhysiological Probesannexin Vcell death

Posted on May 9, 2020
Are Annexin V-FITC+/PI+ cells classified as late-apoptotic cells or necrotic cells?
Chemical ReagentsAV-FITCAnnexin-V StainingApoptosisApoptosis and NecrosisCell ViabilityCell Viability AssaysClassic DyesPropidium iodide (PI)annexin V

Posted on May 9, 2020
Can apoptosis be detected by cell cycle studies?
Chemical ReagentsAnnexin-V StainingApoptosisApoptosis and NecrosisCell ViabilityCell Viability AssaysCellular ProcessesClassic DyesTUNELannexin V

Posted on May 9, 2020
What stain should I use to analyze apoptosis in flow cytometry?
Annexin-V StainingApoptosisApoptosis and NecrosisCell ViabilityCell Viability AssaysClassic DyesFlow Cytometry ReagentsFluoresceinsannexin Vflow cytometry

Posted on May 9, 2020
Which viability test is better: MTT or trypan blue?
Chemical ReagentsCell Cycle and ProliferationCell CytotoxicityCell Membrane IntegrityCell Proliferation AssaysCell ViabilityCell Viability AssaysLive or Dead

Posted on May 9, 2020
What are the best methods to identify necrotic cells?
Antibodies and ProteomicsApoptosisApoptosis and NecrosisCell ViabilityCell Viability AssaysCellular ProcessesDehydrogenaseEnzymesFlow Cytometry Reagentsflow cytometry

Posted on May 9, 2020
What does elevated fluorescence of PI alone in Annexin V/PI staining indicate?
Annexin-V StainingApoptosisApoptosis and NecrosisCell ViabilityCell Viability AssaysFlow Cytometry Reagentsannexin V

Posted on April 24, 2020
What is the difference between ethidium homodimer and dihydroethidium in terms of staining?
Peptide and Oligonucleotide LabelingCell ViabilityDNA and RNA QuantitationDNA and RNAFluorescenceNucleic AcidsPhysiological Probes

Posted on April 17, 2020
Why should a cell die or undergo apoptosis?
ApoptosisApoptosis and NecrosisCell ViabilityCellular Processescell deathFlow Cytometry ReagentsPhysiological Probesflow cytometry

Posted on April 17, 2020
What is the purpose of using cold PBS in the washing step of Annexin V staining?
Chemical ReagentsAnnexin-V StainingApoptosisApoptosis and NecrosisCell MetabolismCell ViabilityCellular ProcessesPBSannexin V

Posted on February 25, 2020
How can I tell if my cell sample is dying?
Cell ViabilityCell countingCell-Impermeable DNA dyes

Posted on February 19, 2020
How do I minimize cell damage during Fluo-4 AM loading?
AM esterCalcium dyesCell ViabilityGreen Fluorescencecalcium indicatorcell damagecell-permeable probesfluo-4

Posted on February 19, 2020
My cells are dying after transfection-what solutions do you recommend?
Cell AssayCell CytotoxicityCell Membrane IntegrityCell ViabilityControlsDNAProteinTransfectioncell culturecell damagecell death

Posted on February 19, 2020
How do I prepare my cells for transfection?
Cell ViabilityTransfectioncell toxicity

Posted on October 22, 2019
Why is determining cell viability important?
Live or Deadlive cell trackinglive cell analysisCell Viability

Posted on October 22, 2019
What are the differences between MTT and WST assays?
AbsorbanceCell ViabilityCell AssayCell CytotoxicityColorimetricWST-8cell proliferation

Posted on August 26, 2019
Why are cells lysed?
Cell AssayCell ViabilityWestern Blotcell lysishomogenouscentrifugeIHCSignal Amplificationantibody labelingimmunohistochemistry

Posted on August 26, 2019
Are NADH and ROS related?
Cell ViabilityNADNADHNAD/NADH RatioNADPNADPHNADP/NADPH ratioROSROS ProbesReactive Oxygen SpeciesTotal ROS probecell damagemitochondria ROS

Posted on August 26, 2019
What does NAD+ do in the body?
Cell ViabilityNAD/NADH RatioNADNADHNADP/NADPH ratioNADPHNADPNADH Assayhuman

Posted on August 19, 2019
What is a WST-8 assay?
WST-8Cell Viabilitylive cell analysiscell proliferationColorimetric

Posted on June 12, 2019
Do you have fixable live cell staining assay kits?
Cell CytotoxicityCell ViabilityMultidrug Resistance (MDR1/P-gp, MRP1)flow cytometryfluorescence imagingfluorescence microplate readerfluorescent microscopefluorescent plate readerlive cell assay

Posted on June 12, 2019
Do you have any cytotoxicity information for your Nuclear LCS dyes?
Cell CytotoxicityPhotostabilityLive or DeadCell ViabilityNuclei Staining

Posted on June 12, 2019
Do you have a ready-to-use cell viability assay kit?
ATPCell Viabilitycell toxicityCell Cytotoxicitychemiluminescenceluminescenseluciferasefirefly luciferaseDTT freeready-to-usehomogenouscell assay