iFluor® conjugated secondary antibodies are powerful tools to identify and visualize target proteins in many applications, including fluorescent cell imaging, immunohistochemistry, flow cytometry, western blotting, and ELISA. The exceptional brightness and photostability of our iFluor® dyes combined with the specificity of our cross-adsorbed goat secondary antibodies guarantee consistency and quality, ensuring bright staining of medium-to-low abundance targets with minimal noise. AAT Bioquest offers secondary antibodies conjugated to 28 unique iFluor® dyes covering the entire spectrum with minimal spectral overlap to accommodate multi-color analysis.
In addition to iFluor dyes, we also offer secondary antibodies conjugated to HRP and poly-HRP, biotin, phycobiliproteins and tandem dyes, Alexa Fluor®
equivalent XFD dyes, and traditional fluorophores (e.g., FITC, Texas Red®
, Cy5, etc.).
iFluor® Secondary Antibodies - Superior Brightness and Photostability
Secondary antibodies labeled with iFluor® dyes exhibit superior brightness, higher signal-to-noise ratios, and improved photostability, outperforming spectrally similar secondary antibody conjugates such as fluorescein and most Alexa Fluor®
secondary antibody conjugates. The excellent photostability and pH insensitivity of our iFluor® secondary antibodies ensure fluorescence intensity remains high over a wide molar range and more extended periods of time Figure 1.
Comparison of photobleaching rates of iFluor® 488 goat anti-mouse IgG (Cat No. 16735
) versus FITC goat anti-mouse IgG (Cat No. 16852
). Images were taken at 120-second intervals on a Keyence X710 fluorescence microscope. TFI represents total fluorescence intensity as calculated by ImageJ software.
Table 1. iFluor® Dyes Spectral Properties