mFluor™ Red 780-streptavidin conjugate

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<p>Flow cytometry analysis of HL-60 cells stained with (Red) or without (Green) 1ug/ml Anti-Human HLA-ABC-Biotin and  then followed by mFluor™ Red 780-streptavidin conjugate (Cat#16948). The fluorescence signal was monitored using ACEA NovoCyte flow cytometer in the APC-Cy7 channel.</p>
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Unit Size: Cat No: Price (USD): Qty:
100 ug 16948 $95


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Overview

SolventWater
Storage Freeze (<-15 °C)
Minimize light exposure
Category mFluor Dyes and Kits,
Dye to Nucleic Acid Conjugation,
Biotin and Streptavidin
Streptavidin conjugates are widely used together with a conjugate of biotin for specific detection of a variety of proteins, protein motifs, nucleic acids and other molecules since streptavidin has a very high binding affinity for biotin. This mFluor™ Red 780-streptavidin conjugate comprises streptavidin (as the biotin-binding protein) with mFluor™ Red 780 covalently attached (as the fluorescent label). It is commonly used as a second step reagent for indirect immunofluorescent staining, when used in conjunction with biotinylated primary antibodies. It is a very valuable tool for biotin-streptavidin-based biological assays and tests using a flow cytometer equiped with a 633 nm excitation (e.g., the Red Laser). A variety of the complementary biotinylated reagents are available from numerous commercial vendors.




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References

Overexpression of MACC1 and the association with hepatocyte growth factor/c-Met in epithelial ovarian cancer
Authors: Hongyu Li, Hui Zhang, Shujun Zhao, Yun Shi, Junge Yao, Yanyan Zhang, Huanhuan Guo, Xingsuo Liu
Journal: Oncology letters (2015): 1989--1996






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