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AAT Bioquest

FAQ

Posted on May 14, 2024
What are the similarities between isothermal amplification and PCR?
Cellular Structures and OrganellesDNA and RNA QuantitationNucleic AcidsNucleusPolymerase Chain Reaction (PCR)


Posted on May 14, 2024
What is nucleic acid amplification?
Cellular Structures and OrganellesDNA and RNA QuantitationNucleic AcidsNucleusPolymerase Chain Reaction (PCR)


Posted on May 14, 2024
What is the significance of nucleic acid amplification?
Cellular Structures and OrganellesDNA and RNA QuantitationNucleic AcidsNucleusPolymerase Chain Reaction (PCR)


Posted on May 10, 2024
What is isothermal amplification?
DNA and RNA QuantitationNucleic AcidsNucleusPolymerase Chain Reaction (PCR)


Posted on May 10, 2024
How does isothermal amplification work?
DNA and RNA QuantitationNucleic AcidsNucleusPolymerase Chain Reaction (PCR)


Posted on May 6, 2024
What is the workflow of the DNA fragment analysis?
DNA and RNA QuantitationDNA FragmentationGene Expression Analysis & GenotypingNucleic AcidsNucleusPolymerase Chain Reaction (PCR)


Posted on May 2, 2024
Is there alternative techniques to Southern blotting?
DNA and RNA QuantitationDNA sequencingPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Sanger SequencingWestern Blot


Posted on May 2, 2024
What are the advantages of southern blot over PCR?
Cellular Structures and OrganellesDNA and RNA QuantitationGene Expression Analysis & GenotypingNucleic AcidsNucleusPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Western Blot


Posted on April 26, 2024
What should I do to optimize the bisulfite PCR conditions for amplifying bisulfite-treated material?
Cellular Structures and OrganellesDNA and RNA QuantitationGene Expression Analysis & GenotypingNucleic AcidsNucleusPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)


Posted on April 24, 2024
How do I know if replication has occurred in my DNA of interest?
Cellular Structures and OrganellesDNA and RNA QuantitationDNA & RNA Markers and LaddersDNA FragmentationGel ElectrophoresisGene Expression Analysis & GenotypingPolymerase Chain Reaction (PCR)


Posted on April 24, 2024
What are the differences between agarose and polyacrylamide?
Cellular Structures and OrganellesDNA and RNA QuantitationDNA FragmentationGel ElectrophoresisPolymerase Chain Reaction (PCR)


Posted on April 24, 2024
What are the similarities between agarose and polyacrylamide?
Cellular Structures and OrganellesDNA and RNA QuantitationGel ElectrophoresisNucleic AcidsNucleusPolymerase Chain Reaction (PCR)


Posted on April 24, 2024
What are the advantages of agarose gel?
Cellular Structures and OrganellesDNA and RNA QuantitationGel ElectrophoresisNucleic AcidsNucleusPolymerase Chain Reaction (PCR)


Posted on April 24, 2024
What are the disadvantages of agarose gel?
Cellular Structures and OrganellesDNA and RNA QuantitationGel ElectrophoresisNucleic AcidsNucleusPolymerase Chain Reaction (PCR)


Posted on April 24, 2024
What are the advantages of polyacrylamide gel?
Cellular Structures and OrganellesDNA and RNA QuantitationGel ElectrophoresisNucleic AcidsNucleusPolymerase Chain Reaction (PCR)


Posted on April 24, 2024
What are the disadvantages of polyacrylamide gel?
Cellular Structures and OrganellesDNA and RNA QuantitationGel ElectrophoresisNucleic AcidsNucleusPolymerase Chain Reaction (PCR)


Posted on April 24, 2024
How does protein electrophoresis differ from DNA electrophoresis?
Cellular Structures and OrganellesDNA and RNA QuantitationGel ElectrophoresisNucleic AcidsNucleusPolymerase Chain Reaction (PCR)


Posted on April 24, 2024
What are the similarities between horizontal and vertical gel electrophoresis?
Cellular Structures and OrganellesDNA and RNA QuantitationGel ElectrophoresisNucleic AcidsNucleusPolymerase Chain Reaction (PCR)


Posted on April 24, 2024
What are the differences between horizontal and vertical gel electrophoresis?
Cellular Structures and OrganellesDNA and RNA QuantitationGel ElectrophoresisNucleic AcidsNucleusPolymerase Chain Reaction (PCR)


Posted on April 24, 2024
How do I analyze gel electrophoresis?
Cellular Structures and OrganellesDNA and RNA QuantitationDNA & RNA Markers and LaddersDNA FragmentationGel ElectrophoresisNucleic AcidsNucleusPolymerase Chain Reaction (PCR)


Posted on April 24, 2024
Why is it important to mix samples/ladders with loading dyes when setting up gel electrophoresis?
Cellular Structures and OrganellesDNA and RNA QuantitationGel ElectrophoresisNucleic AcidsNucleusPolymerase Chain Reaction (PCR)


Posted on April 24, 2024
What are the buffers of choice for gel electrophoresis?
Cellular Structures and OrganellesDNA and RNA QuantitationGel ElectrophoresisNucleic AcidsNucleusPolymerase Chain Reaction (PCR)


Posted on April 24, 2024
Why is TAE the preferred choice for gel electrophoresis?
Cellular Structures and OrganellesDNA and RNA QuantitationGel ElectrophoresisNucleic AcidsNucleusPolymerase Chain Reaction (PCR)


Posted on April 18, 2024
What are the common challenges in sample preparation for NGS?
DNA and RNA QuantitationGene Expression Analysis & GenotypingNucleic AcidsNucleusPolymerase Chain Reaction (PCR)Sanger Sequencing


Posted on April 18, 2024
What is a typical method of DNA library preparation for NGS?
DNA and RNA QuantitationGene Expression Analysis & GenotypingNucleic AcidsNucleusPolymerase Chain Reaction (PCR)Sanger Sequencing


Posted on April 18, 2024
What techniques can be used to determine the number of nucleic acids present in an NGS library?
DNA and RNA QuantitationGene Expression Analysis & GenotypingNucleic AcidsNucleusPolymerase Chain Reaction (PCR)Sanger Sequencing


Posted on March 18, 2024
What methods are used to detect miRNAs?
DNA and RNA QuantitationGene Expression Analysis & GenotypingNucleic AcidsNucleusPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)


Posted on March 14, 2024
What are the similarities between introns and exons?
Antibodies and Proteomics BioconjugationCellular ProcessesDNA and RNA QuantitationNucleic AcidsNucleusPolymerase Chain Reaction (PCR)Sanger Sequencing


Posted on March 14, 2024
What are the differences between introns and exons?
Antibodies and ProteomicsCellular ProcessesDNA and RNA QuantitationNucleic AcidsNucleusPolymerase Chain Reaction (PCR)Sanger Sequencing


Posted on March 11, 2024
What are the advantages of second generation NGS?
DNA and RNA QuantitationDNA sequencingGene Expression Analysis & GenotypingPolymerase Chain Reaction (PCR)Sanger Sequencinggene sequencing


Posted on March 11, 2024
What are the disadvantages of second generation NGS?
DNA and RNA QuantitationDNA sequencingGene Expression Analysis & GenotypingPolymerase Chain Reaction (PCR)Sanger Sequencinggene sequencing


Posted on March 11, 2024
What are the advantages of third generation NGS?
DNA and RNA QuantitationDNA sequencingGene Expression Analysis & GenotypingPolymerase Chain Reaction (PCR)Sanger Sequencinggene sequencing


Posted on February 28, 2024
What is nested PCR?
DNA and RNA QuantitationNucleic AcidsNucleusPolymerase Chain Reaction (PCR)


Posted on February 28, 2024
What is the principle of Nested PCR?
DNA and RNA QuantitationNucleic AcidsNucleusPolymerase Chain Reaction (PCR)


Posted on February 28, 2024
What are the applications of nested PCR?
DNA and RNA QuantitationNucleic AcidsNucleusPolymerase Chain Reaction (PCR)


Posted on February 28, 2024
What are the advantages of nested PCR?
DNA and RNA QuantitationNucleic AcidsNucleusPolymerase Chain Reaction (PCR)


Posted on February 28, 2024
What are the limitations of nested PCR?
DNA and RNA QuantitationNucleic AcidsNucleusPolymerase Chain Reaction (PCR)


Posted on February 28, 2024
What are the similarities between real time PCR and nested PCR?
DNA and RNA QuantitationNucleic AcidsNucleusPolymerase Chain Reaction (PCR)


Posted on February 28, 2024
What are the differences between real time PCR and nested PCR?
DNA and RNA QuantitationNucleic AcidsNucleusPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)


Posted on February 28, 2024
What are the functions of MgCI2 in a PCR reaction?
DNA and RNA QuantitationNucleic AcidsNucleusPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)


Posted on February 28, 2024
Why is MgCI2 used in the PCR?
DNA and RNA QuantitationNucleic AcidsNucleusPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)


Posted on February 28, 2024
When do I need to add some extra amount of MgCI2?
DNA and RNA QuantitationNucleic AcidsNucleusPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)


Posted on February 28, 2024
What is the ideal concentration of MGCI2 in a PCR reaction?
DNA and RNA QuantitationNucleic AcidsNucleusPolymerase Chain Reaction (PCR)


Posted on February 28, 2024
What is multiplex PCR?
DNA and RNA QuantitationNucleic AcidsNucleusPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)


Posted on February 28, 2024
What is the principle of multiplex PCR?
DNA and RNA QuantitationNucleic AcidsNucleusPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)


Posted on February 28, 2024
What are the advantages of multiplex PCR?
DNA and RNA QuantitationNucleic AcidsNucleusPolymerase Chain Reaction (PCR)


Posted on February 28, 2024
What are the limitations of multiplex PCR?
DNA and RNA QuantitationNucleic AcidsNucleusPolymerase Chain Reaction (PCR)


Posted on February 28, 2024
What are the applications of multiplex PCR?
DNA and RNA QuantitationNucleic AcidsNucleusPolymerase Chain Reaction (PCR)


Posted on February 28, 2024
What factors should I consider when designing amplification primers for multiplex PCR?
DNA and RNA QuantitationNucleic AcidsNucleusPolymerase Chain Reaction (PCR)


Posted on February 28, 2024
How does the immune system tell self from non-self?
DNA and RNA QuantitationNucleic AcidsNucleusPolymerase Chain Reaction (PCR)


Posted on February 20, 2024
What are the components required to set up the chain termination PCR?
DNA and RNA QuantitationDNA sequencingNucleic AcidsNucleusPolymerase Chain Reaction (PCR)Sanger Sequencing


Posted on February 20, 2024
What does each component in chain termination PCR do?
DNA and RNA QuantitationDNA sequencingNucleic AcidsNucleusPolymerase Chain Reaction (PCR)Sanger Sequencing


Posted on February 20, 2024
What are the different techniques commonly used to amplify DNA fragments before sequencing?
DNA and RNA QuantitationDNA sequencingNucleic AcidsNucleusPolymerase Chain Reaction (PCR)Sanger Sequencing


Posted on November 11, 2023
What are the advantages of digital PCR (dPCR)?
PCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Tide Quencher DyesDigital PCR


Posted on November 9, 2023
What are the disadvantages of end-point RT-PCR?
PCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Reverse Transcription PCR (RT-PCR)


Posted on November 8, 2023
What are the advantages of end-point reverse transcription polymerase chain reaction (RT-PCR)?
PCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Reverse Transcription PCR (RT-PCR)


Posted on November 8, 2023
What are the basic principles of real-time PCR?
Gene Expression Analysis & GenotypingPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)


Posted on November 8, 2023
What are the major differences between qPCR and conventional PCR?
PCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on November 7, 2023
What are the applications of immunochemical techniques?
Antibodies and Proteomics Bioconjugation Chemical Reagents Enzyme-Linked Immunosorbent Assay (ELISA)Cell SignalingCellular ProcessesImmunohistochemistry (IHC)Polymerase Chain Reaction (PCR)Western Blot


Posted on September 18, 2023
How are microbes identified?
BioconjugationCellular ProcessesCellular Structures and OrganellesMycoLight Dyes and KitsPhysiological ProbesPolymerase Chain Reaction (PCR)bacteria


Posted on September 18, 2023
What are the common medical microbiology techniques?
Cell CultureCell Cycle and ProliferationCellular Structures and OrganellesPolymerase Chain Reaction (PCR)


Posted on August 29, 2023
What are molecular beacons used for?
Peptide and Oligonucleotide LabelingDNA and RNA QuantitationNucleic AcidsPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)


Posted on August 29, 2023
How does a molecular beacon work?
Förster Resonance Energy Transfer (FRET) Peptide and Oligonucleotide LabelingNucleic AcidsPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Tide Quencher Dyes


Posted on August 29, 2023
What is the importance of molecular beacons?
Förster Resonance Energy Transfer (FRET) Peptide and Oligonucleotide LabelingDNA and RNA QuantitationNucleic AcidsPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Tide Quencher Dyes


Posted on August 29, 2023
What are the advantages of molecular beacon probes?
Förster Resonance Energy Transfer (FRET) Peptide and Oligonucleotide LabelingDNA and RNA QuantitationNucleic AcidsPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Tide Quencher Dyes


Posted on August 29, 2023
What are the limitations of molecular beacon probes?
Förster Resonance Energy Transfer (FRET) Peptide and Oligonucleotide LabelingDNA and RNA QuantitationNucleic AcidsNucleusPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Tide Quencher Dyes


Posted on August 29, 2023
Why are MBs particularly suited for mRNA studies?
Förster Resonance Energy Transfer (FRET) Peptide and Oligonucleotide LabelingDNA and RNA QuantitationNucleic AcidsPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Tide Quencher Dyes


Posted on August 29, 2023
What are the strategies for minimizing MB false-positive signals in living cells?
Förster Resonance Energy Transfer (FRET) Peptide and Oligonucleotide LabelingDNA and RNA QuantitationNucleic AcidsPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Tide Quencher Dyes


Posted on August 29, 2023
What are the properties of molecular beacons that affect nucleic acid detection?
Förster Resonance Energy Transfer (FRET) Peptide and Oligonucleotide LabelingDNA and RNA QuantitationNucleic AcidsPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Tide Quencher Dyes


Posted on August 29, 2023
What are the widely used detection methods in quantitative PCR (Q-PCR)
Förster Resonance Energy Transfer (FRET) Peptide and Oligonucleotide LabelingDNA and RNA QuantitationNucleic AcidsPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Tide Quencher Dyes


Posted on August 29, 2023
What are the steps of a molecular beacon RT-PCR assay?
Peptide and Oligonucleotide LabelingDNA and RNA QuantitationPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)Tide Quencher Dyes


Posted on August 29, 2023
What are the best ways to store a molecular beacon preparation?
Förster Resonance Energy Transfer (FRET) Peptide and Oligonucleotide LabelingDNA and RNA QuantitationNucleic AcidsPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)


Posted on August 29, 2023
What are the reasons for the low signal-to-background ratio after the synthesis and purification of a molecular beacon and how do I solve the problem?
Peptide and Oligonucleotide LabelingDNA and RNA QuantitationNucleic AcidsPolymerase Chain Reaction (PCR)PurificationReal-Time PCR (qPCR)


Posted on August 14, 2023
Why is thermostable DNA polymerase required for PCR?
DNA and RNA QuantitationGel ElectrophoresisNucleic AcidsNucleusPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on August 14, 2023
What are the applications of PCR?
DNA and RNA QuantitationNucleic AcidsNucleusPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on August 14, 2023
What does Taq polymerase do in PCR?
DNA and RNA QuantitationNucleic AcidsNucleusPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on August 14, 2023
What do I do if my PCR isn't working?
DNA and RNA QuantitationNucleic AcidsNucleusPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on August 14, 2023
What does Taq buffer do in PCR?
DNA and RNA QuantitationNucleic AcidsNucleusPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on August 14, 2023
What do forward and reverse primers do in PCR?
DNA and RNA QuantitationNucleic AcidsNucleusPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on August 14, 2023
What are the similarities between forward and reverse primers?
DNA and RNA QuantitationNucleic AcidsNucleusPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on August 14, 2023
What are the steps involved in getting the reverse primer sequence?
DNA and RNA QuantitationNucleic AcidsNucleusPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on August 14, 2023
What are the guidelines should I follow when designing primers?
DNA and RNA QuantitationNucleic AcidsNucleusPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on August 14, 2023
Why is it necessary to purify PCR products?
DNA and RNA QuantitationNucleic AcidsNucleusPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on August 14, 2023
Why are quenchers used in real time PCR?
DNA and RNA QuantitationNucleic AcidsNucleusPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Tide Quencher Dyes


Posted on August 14, 2023
When should I use one-step as opposed to two-step qRT-PCR?
DNA and RNA QuantitationNucleic AcidsNucleusPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Tide Quencher Dyes


Posted on August 14, 2023
When should I use dPCR to quantify NGS libraries?
DNA and RNA QuantitationPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)Tide Quencher Dyesgene sequencing


Posted on August 10, 2023
What is the basic principle behind RNA-Seq technology?
Cellular Structures and OrganellesDNA and RNA QuantitationNucleic AcidsNucleusPolymerase Chain Reaction (PCR)gene sequencing


Posted on August 9, 2023
How can I access and analyze a publicly available RNA-seq database?
Cellular Structures and OrganellesDNA and RNA QuantitationNucleic AcidsNucleusPolymerase Chain Reaction (PCR)gene sequencing


Posted on August 9, 2023
How do I prepare an RNA sequencing library?
Cellular Structures and OrganellesDNA and RNA QuantitationNucleic AcidsNucleusPolymerase Chain Reaction (PCR)gene sequencing


Posted on August 9, 2023
What are the challenges of RNA-Seq technology?
Cellular Structures and OrganellesDNA and RNA QuantitationNucleic AcidsNucleusPolymerase Chain Reaction (PCR)gene sequencing


Posted on August 9, 2023
Why is RNA-seq considered superior to microarrays?
DNA and RNA QuantitationNucleic AcidsNucleusPolymerase Chain Reaction (PCR)gene sequencing


Posted on August 9, 2023
What are some challenges and solutions in single cell RNA-Seq data analysis?
Cellular Structures and OrganellesDNA and RNA QuantitationNucleic AcidsNucleusPolymerase Chain Reaction (PCR)gene sequencing


Posted on August 9, 2023
What are the key steps involved in RNA-Seq?
Cellular Structures and OrganellesDNA and RNA QuantitationNucleic AcidsNucleusPolymerase Chain Reaction (PCR)


Posted on August 5, 2023
How are recombinant proteins made?
Antibodies and Proteomics BioconjugationDNA and RNAPolymerase Chain Reaction (PCR)ProteinTransfection


Posted on August 4, 2023
What’s the significance of ROX reference dye in real time PCR (qPCR)?
Chemical ReagentsCell Structures and OrganellsPhysiological ProbesPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)


Posted on August 3, 2023
What is removed during mRNA processing?
DNA and RNA QuantitationNucleic AcidsNucleusPolymerase Chain Reaction (PCR)Reverse Transcription PCR (RT-PCR)


Posted on August 3, 2023
How is transcribed mRNA processed in eukaryotes?
Antibodies and ProteomicsDNA and RNA QuantitationNucleic AcidsNucleusPolymerase Chain Reaction (PCR)Reverse Transcription PCR (RT-PCR)


Posted on July 10, 2023
How does protein synthesis occur in eukaryotes?
Cell SignalingCellular ProcessesDNA and RNA QuantitationDNA and RNAPolymerase Chain Reaction (PCR)Protein


Posted on May 20, 2023
Is magnesium chloride an acid or base?
DNA and RNA QuantitationDNA and RNANucleic AcidsPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on May 20, 2023
Is magnesium chloride soluble in organic solvents?
DNA and RNA QuantitationDNA and RNAPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)magnesium


Posted on May 20, 2023
How does magnesium concentration affect PCR?
DNA and RNA QuantitationDNA and RNANucleic AcidsPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on May 20, 2023
What is the ideal concentration of MgCl2?
DNA and RNA QuantitationDNA and RNAPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on May 20, 2023
What is the mechanism of MgCl2 during PCR amplification?
DNA and RNA QuantitationDNA and RNANucleic AcidsPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on May 20, 2023
Why is isothermal amplification not as commonly used as PCR?
DNA and RNA QuantitationDNA and RNANucleic AcidsPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on May 20, 2023
When should I use isothermal amplification instead of PCR?
DNA and RNA QuantitationDNA and RNANucleic AcidsPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on May 20, 2023
What are the advantages of isothermal amplification?
DNA and RNA QuantitationDNA and RNANucleic AcidsPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on May 20, 2023
What are the differences between isothermal amplification and PCR?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on May 20, 2023
What are the alternative methods of polymerase chain reaction (PCR)?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on May 19, 2023
How do I inactivate proteinase K?
Cellular ProcessesEnzymesPolymerase Chain Reaction (PCR)ProteasesProtein


Posted on May 19, 2023
What are the applications of proteinase K?
Cellular ProcessesDNA and RNAEnzymesPolymerase Chain Reaction (PCR)Protein


Posted on May 12, 2023
What are the differences between forward primer and reverse primer?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCRPCR primerPCR stepsPolymerase Chain Reaction (PCR)Types of PCR


Posted on May 12, 2023
What is the principle of PCR?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCRPCR primerPCR stepsPolymerase Chain Reaction (PCR)Types of PCR


Posted on May 12, 2023
What are the similarities between primers and promotors?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCR Detection of Viral DNA/RNAPCR primerPCR stepsPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)Types of PCRgene sequencing


Posted on May 12, 2023
What are the differences between primers and promotors?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCR Detection of Viral DNA/RNAPCR primerPCR stepsPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)Types of PCR


Posted on May 12, 2023
How does Real-Time PCR work?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCRPCR Detection of Viral DNA/RNAPCR primerPCR stepsPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)Types of PCR


Posted on May 12, 2023
What are the advantages of Real-time PCR over PCR?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCR Detection of Viral DNA/RNAPCR primerPCR stepsPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)Types of PCR


Posted on May 12, 2023
What is the workflow of real-time PCR?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCRPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)Types of PCR


Posted on May 12, 2023
What are the methods used to measure the DNA application in qPCR?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)Types of PCR


Posted on May 12, 2023
Should I use probe-based detection or dye-based detection for my qPCR analysis?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)Types of PCR


Posted on May 12, 2023
What are the factors I should consider to get accurate and reproducible results for my probe-based qPCR?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on May 12, 2023
What are the advantages of probe-based real-time PCR?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on May 12, 2023
What is the principle of probe-based qPCR?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCRPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on May 12, 2023
How do I design primers for qPCR?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCRPCR Detection of Viral DNA/RNAPCR stepsPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on May 12, 2023
What should I consider when I set up a PCR efficiency test for my assay?
DNA and RNA QuantitationDNA and RNAPCRPCR Detection of Viral DNA/RNAPCR primerPCR stepsPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on May 12, 2023
What is the gold standard for gene quantification?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCRPCR Detection of Viral DNA/RNAPCR stepsPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on May 12, 2023
What's the significance of reference genes?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCRPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)gene sequencing


Posted on May 12, 2023
What is the significance of the cycle threshold?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCRPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)Types of PCR


Posted on May 12, 2023
What are the advantages of multiplexing techniques in real time PCR (qPCR)?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCRPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on May 12, 2023
What factors should I consider when I choose fluorophore/quencher combinations for my multiplex qPCR reactions?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCRPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on May 12, 2023
What are the steps involved in probe-based qPCR?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCRPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)Types of PCR


Posted on May 2, 2023
What are features of next generation sequencing?
DNADNA and RNADNA sequencingNucleic AcidsNucleusPCRPolymerase Chain Reaction (PCR)Sanger Sequencinggene sequencing


Posted on May 2, 2023
What are the limitations of Sanger sequencing?
DNADNA and RNANucleic AcidsNucleusPCRPolymerase Chain Reaction (PCR)Sanger Sequencinggene sequencing


Posted on May 2, 2023
Which technique is used to visualize DNA following a PCR reaction?
Agarose gel electrophoresisDNADNA and RNAGel ElectrophoresisNucleic AcidsNucleusPCRPolymerase Chain Reaction (PCR)Proteinpolyacrylamide geldna ladder


Posted on May 2, 2023
What factors should I consider before I use restriction enzymes to cut DNA into small fragments?
DNA and RNA QuantitationDNA and RNAEnzymesNucleic AcidsNucleusPCRPolymerase Chain Reaction (PCR)


Posted on April 27, 2023
What happens if I add too much MgCI2 in my PCR reaction?
DNADNA and RNA QuantitationDNA and RNAPCRPCR primerPolymerase Chain Reaction (PCR)magnesium


Posted on April 27, 2023
What happens if I add too little MgCI2 in my PCR reaction?
DNADNA and RNA QuantitationDNA and RNAEnzymesPCR primerPolymerase Chain Reaction (PCR)


Posted on April 27, 2023
In PCR, what are the functions of DMSO?
DMSODNADNA and RNA QuantitationDNA and RNAPCRPCR primerPolymerase Chain Reaction (PCR)


Posted on March 1, 2023
What is the end product of transcription?
DNA and RNA QuantitationDNA and RNAEnzymesNucleic AcidsNucleusPolymerase Chain Reaction (PCR)PolymerasesReverse Transcription PCR (RT-PCR)


Posted on March 1, 2023
What are the differences between cDNA and genomic DNA library?
DNA and RNA QuantitationDNA and RNAGenomic DNANucleic AcidsPolymerase Chain Reaction (PCR)Reverse Transcription PCR (RT-PCR)


Posted on March 1, 2023
What is the significance of cDNA?
DNA and RNA QuantitationDNA and RNANucleic AcidsPolymerase Chain Reaction (PCR)Reverse Transcription PCR (RT-PCR)


Posted on March 1, 2023
What is the workflow of cDNA library construction?
DNA and RNA QuantitationDNA and RNANucleic AcidsPolymerase Chain Reaction (PCR)Reverse Transcription PCR (RT-PCR)


Posted on March 1, 2023
What are the advantages of cDNA library?
DNA and RNA QuantitationDNA and RNANucleic AcidsPolymerase Chain Reaction (PCR)Reverse Transcription PCR (RT-PCR)


Posted on March 1, 2023
What are the disadvantages of cDNA library?
DNA and RNA QuantitationDNA and RNANucleic AcidsPolymerase Chain Reaction (PCR)Reverse Transcription PCR (RT-PCR)


Posted on March 1, 2023
What are the steps to construct a genomic DNA library?
DNA and RNA QuantitationDNA and RNAEnzymesGenomic DNANucleic AcidsPolymerase Chain Reaction (PCR)Reverse Transcription PCR (RT-PCR)


Posted on March 1, 2023
What are the applications of cDNA library?
DNA and RNA QuantitationDNA and RNANucleic AcidsPolymerase Chain Reaction (PCR)Reverse Transcription PCR (RT-PCR)


Posted on March 1, 2023
What is the procedure of expression screening?
DNA and RNA QuantitationDNA and RNAGel ElectrophoresisPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)Western Blot


Posted on March 1, 2023
Where does the transcription terminate?
DNA and RNA QuantitationDNA and RNANucleic AcidsPolymerase Chain Reaction (PCR)PolymerasesReverse Transcription PCR (RT-PCR)


Posted on March 1, 2023
Are enhancers necessary for transcription?
DNA and RNA QuantitationDNA and RNANucleic AcidsPolymerase Chain Reaction (PCR)Reverse Transcription PCR (RT-PCR)


Posted on March 1, 2023
What are the promoter sequences?
DNA and RNA QuantitationDNA and RNANucleic AcidsPolymerase Chain Reaction (PCR)Reverse Transcription PCR (RT-PCR)


Posted on March 1, 2023
What are the common primers used in reverse transcription?
DNA and RNA QuantitationDNA and RNAPCR primerPeptide and OligonucleotidePolymerase Chain Reaction (PCR)RNAReverse Transcription PCR (RT-PCR)


Posted on March 1, 2023
What are the reaction components for reverse transcription?
DNA and RNA QuantitationDNA and RNAEnzymesNucleic AcidsPCR Detection of Viral DNA/RNAPCR primerPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)bufferdNTPsinhibitor


Posted on March 1, 2023
How do I optimize my reverse transcription process?
DNA and RNA QuantitationDNA and RNAGenomic DNANucleic AcidsPCR Detection of Viral DNA/RNAPCR primerPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on February 23, 2023
What are the applications for reverse transcriptase?
DNA and RNA QuantitationDNA and RNAPolymerase Chain Reaction (PCR)Reverse Transcription PCR (RT-PCR)


Posted on February 17, 2023
What are the differences between Taq polymerase and DNA polymerase?
DNA and RNA QuantitationDNA and RNAEnzymesNucleic AcidsNucleusPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)PolymerasesReal-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on February 17, 2023
What are the properties of Taq polymerase enzymes?
DNA and RNA QuantitationDNA and RNAEnzymesNucleic AcidsNucleusPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)PolymerasesReal-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on February 17, 2023
What is the optimal magnesium concentration for Taq polymerase?
DNA and RNA QuantitationDNA and RNAEnzymesNucleic AcidsNucleusPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)PolymerasesReal-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)magnesium


Posted on February 17, 2023
What happens if I use higher primer concentrations?
PCR Detection of Viral DNA/RNAPCR primerPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on February 17, 2023
What is the typical concentration for dNTPs?
DNA and RNA QuantitationDNA and RNAPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on February 17, 2023
Does PCR use DNA or RNA primers?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on February 17, 2023
What are the properties of a primer?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on February 17, 2023
How do I remove the RNA primers?
DNA and RNAEnzymesPolymerase Chain Reaction (PCR)Polymerases


Posted on February 17, 2023
What happens if I only use a forward primer?
DNA and RNAPCR primerPolymerase Chain Reaction (PCR)


Posted on February 13, 2023
What are the steps of PCR amplification?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on February 13, 2023
What are the advantages of PCR (polymerase chain reaction)?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCRPolymerase Chain Reaction (PCR)


Posted on February 13, 2023
What are the common PCR additives?
BSADMSODNA and RNA QuantitationDNA and RNAPolymerase Chain Reaction (PCR)bovine serum albumindetergentmagnesium


Posted on February 13, 2023
What are the components required for PCR reaction?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on February 13, 2023
How should oligos be purified for PCR?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on February 13, 2023
How does PCR work?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on February 13, 2023
What is PCR used for?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on February 13, 2023
How do I find the concentration of a primer?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCR Detection of Viral DNA/RNAPCR primerPolymerase Chain Reaction (PCR)


Posted on February 13, 2023
What primer concentration should I use for PCR?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)


Posted on January 30, 2023
How do bacterial ribosome binding sites work?
Cell Structures and OraganellesDNA and RNA QuantitationNucleusPhysiological ProbesPolymerase Chain Reaction (PCR)


Posted on January 30, 2023
What are the properties of glycine?
Bioconjugation Chemical ReagentsClassic DyesPolymerase Chain Reaction (PCR)


Posted on January 27, 2023
Is glycine a weak acid?
Bioconjugation Chemical ReagentsClassic DyesPolymerase Chain Reaction (PCR)


Posted on January 23, 2023
What are the similarities between promoters and enhancers?
DNA and RNA QuantitationDNA and RNADNA sequencingPolymerase Chain Reaction (PCR)


Posted on January 23, 2023
How do I mutate plasmids?
BioconjugationEnzymesNucleic AcidsPolymerase Chain Reaction (PCR)


Posted on January 18, 2023
How much DMSO do I add to PCR?
DMSODNA and RNA QuantitationDNA and RNAPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on January 18, 2023
What are the steps of emulsion PCR?
DNA and RNA QuantitationDNA and RNADNA sequencingPhysiological ProbesPolymerase Chain Reaction (PCR)


Posted on January 18, 2023
How can I avoid primer-dimer formation during PCR amplification?
DNA and RNA QuantitationDNA and RNAPhysiological ProbesPolymerase Chain Reaction (PCR)


Posted on January 13, 2023
What are the differences between static quenching and dynamic quenching?
Bioconjugation Chemical Reagents Förster Resonance Energy Transfer (FRET)Classic DyesPhysiological ProbesPolymerase Chain Reaction (PCR)Tide Quencher Dyes


Posted on January 13, 2023
What is the mechanism of dynamic quenching?
Bioconjugation Chemical Reagents Förster Resonance Energy Transfer (FRET)Classic DyesFluorescencePhysiological ProbesPolymerase Chain Reaction (PCR)Tide Quencher Dyes


Posted on January 13, 2023
What are the types of fluorescence quenching?
Bioconjugation Chemical Reagents Förster Resonance Energy Transfer (FRET)Classic DyesFluorescencePhysiological ProbesPolymerase Chain Reaction (PCR)Tide Quencher Dyes


Posted on January 9, 2023
Where are bound ribosomes located?
Cell Structures and OraganellesEndoplasmic Reticulum (ER)Physiological ProbesPolymerase Chain Reaction (PCR)


Posted on January 6, 2023
What are the differences between eukaryotic transcription and prokaryotic transcription?
Cellular Structures and OrganellesDNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPhysiological ProbesPolymerase Chain Reaction (PCR)


Posted on November 14, 2022
What are the similarities between Fluorophore and Chromophore?
Bioconjugation Chemical Reagents Förster Resonance Energy Transfer (FRET)DNA and RNA QuantitationDyes by Functional GroupPCRPhysiological ProbesPolymerase Chain Reaction (PCR)


Posted on November 14, 2022
What is the difference between Black Hole Quencher (BHQ) dye and TAMRA?
Bioconjugation Chemical ReagentsClassic DyesDNA and RNA QuantitationFluorescencePCRPhysiological ProbesPolymerase Chain Reaction (PCR)


Posted on October 22, 2022
Is RNA editing better than DNA editing?
Cell Structures and OrganellesDNA and RNA QuantitationNucleusPhysiological ProbesPolymerase Chain Reaction (PCR)


Posted on October 22, 2022
How do I detect alternative splicing?
NucleusPCR Detection of Viral DNA/RNAPhysiological ProbesPolymerase Chain Reaction (PCR)


Posted on September 19, 2022
What is SDS-PAGE?
Chemical ReagentsAgarose gel electrophoresisDNA and RNADNA sequencingGel ElectrophoresisPolymerase Chain Reaction (PCR)detergentpolyacrylamide gel


Posted on September 19, 2022
Why is SDS used in protein gel electrophoresis?
Agarose gel electrophoresisDNA and RNADNA sequencingGel ElectrophoresisPolymerase Chain Reaction (PCR)Western Blotdetergentgene sequencingpolyacrylamide gel


Posted on September 19, 2022
What is the purpose of preparing a master mix?
Cell Structures and OrganellesDNA and RNA QuantitationPhysiological ProbesPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)


Posted on August 31, 2022
What is a crosslinked polymer?
Antibody and Protein LabelingBioconjugationPolymerase Chain Reaction (PCR)PolymerasesProtein to Protein Conjugation


Posted on June 21, 2022
What are the advantages of RT-LAMP technology?
Polymerase Chain Reaction (PCR)DNA and RNA QuantitationPhysiological ProbesCell Structures and Organelles


Posted on June 21, 2022
How do I design LAMP primers?
Cell Structures and OrganellesDNA and RNA QuantitationPhysiological ProbesPolymerase Chain Reaction (PCR)


Posted on June 21, 2022
How many primers are used in the LAMP method?
Polymerase Chain Reaction (PCR)DNA and RNA QuantitationPhysiological Probes


Posted on March 22, 2022
What factors affect the specificity of PCR?
BioconjugationPhysiological ProbesPolymerase Chain Reaction (PCR)DNA and RNA Quantitation


Posted on March 3, 2022
What are the differences between a standard PCR and RT PCR?
Reverse Transcription PCR (RT-PCR)Polymerase Chain Reaction (PCR)Chemical ReagentsClassic DyesDNA and RNA Quantitation


Posted on February 9, 2022
What are the common applications of PCR?
Polymerase Chain Reaction (PCR)Coronavirus (SARS-CoV-2)Real-Time PCR (qPCR)DNA and RNA Quantitation


Posted on February 8, 2022
Does PCR use polymerase?
Physiological ProbesPolymerase Chain Reaction (PCR)PolymerasesDNA and RNA Quantitation


Posted on February 4, 2022
How does temperature affect PCR?
Polymerase Chain Reaction (PCR)DNA and RNA QuantitationPhysiological ProbesCell Structures and OrganellesNucleus


Posted on February 4, 2022
Why are the PCR reagents stored on ice at all times?
Polymerase Chain Reaction (PCR)Cell Structures and OrganellesNucleusDNA and RNA QuantitationPhysiological Probes


Posted on December 16, 2020
What is qPCR?
DNA and RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)qPCR


Posted on December 5, 2020
What is reverse transcription PCR?
DNA and RNA QuantitationDNA and RNAPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on November 23, 2020
What is PCR amplification?
DNA and RNA QuantitationDNA and RNANucleic AcidsPhysiological ProbesPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on September 30, 2020
What is the best DNA extraction method for genomics applications?
DNADNA and RNA QuantitationDNA and RNADNA extractionPolymerase Chain Reaction (PCR)


Posted on July 22, 2020
What methods are used in next-generation sequencing (NGS) to amplify templates?
DNADNA and RNADNA sequencingPolymerase Chain Reaction (PCR)gene sequencing


Posted on July 22, 2020
What methods are commonly used to label the probes in fluorescence in situ hybridization (FISH)?
DNA and RNAEnzymesFISHFluorescencePeptide and OligonucleotidePolymerase Chain Reaction (PCR)in situ hybridization


Posted on July 22, 2020
What are the general steps of fluorescence in situ hybridization (FISH) experiments?
DNADNA and RNAFISHFluorescencePolymerase Chain Reaction (PCR)RNAin situ hybridizationssDNA


Posted on July 22, 2020
How do you prepare probes for fluorescence in situ hybridization (FISH)?
DNA and RNAEnzymesFISHFluorescencePolymerase Chain Reaction (PCR)in situ hybridization


Posted on July 15, 2020
6-ROX is predominately used as a reference dye for performing PCR detections, however, 6-ROX is very unstable compared to other rhodamine dyes. What is a better dye replacement?
Chemical ReagentsDNA and RNADNA and RNA QuantitationFluorescencePCRPhysiological ProbesPolymerase Chain Reaction (PCR)


Posted on June 22, 2020
What is the difference between transcription and reverse transcription?
DNA and RNA QuantitationDNA and RNAPCRPhysiological ProbesPolymerase Chain Reaction (PCR)PolymerasesReal-Time PCR (qPCR)


Posted on June 22, 2020
What are the general steps of transcription?
DNA and RNA QuantitationDNA and RNAPCRPolymerase Chain Reaction (PCR)PolymerasesReal-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on June 22, 2020
Are all DNA molecules protein-coding?
DNADNA and RNANucleic AcidsPolymerase Chain Reaction (PCR)ProteinRNAReal-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on June 22, 2020
What are the functions of introns?
DNADNA and RNANucleic AcidsPCRPolymerase Chain Reaction (PCR)RNA


Posted on June 22, 2020
What is RNA splicing?
DNA and RNANucleic AcidsPolymerase Chain Reaction (PCR)ProteinRNA


Posted on June 22, 2020
What are the three major steps in mRNA processing?
DNADNA and RNAPolymerase Chain Reaction (PCR)ProteinRNA


Posted on June 22, 2020
What is the function of the 5’ cap and 3’ poly-A tail in mRNA?
DNADNA and RNAPolymerase Chain Reaction (PCR)ProteinRNA


Posted on June 22, 2020
What are exons?
DNADNA and RNANucleic AcidsPolymerase Chain Reaction (PCR)ProteinRNA


Posted on June 22, 2020
What is translation in biology?
Cellular ProcessesDNA and RNANucleic AcidsPolymerase Chain Reaction (PCR)ProteinRNA


Posted on June 22, 2020
What is recombinant DNA?
DNADNA and RNANucleic AcidsPolymerase Chain Reaction (PCR)


Posted on June 22, 2020
What is molecular cloning?
Cellular ProcessesDNADNA and RNANucleic AcidsPolymerase Chain Reaction (PCR)


Posted on June 22, 2020
What is the difference between PCR and molecular cloning regarding DNA replication?
DNADNA and RNANucleic AcidsPCRPolymerase Chain Reaction (PCR)


Posted on June 22, 2020
What are the main steps of cloning?
DNA and RNANucleic AcidsPCRPolymerase Chain Reaction (PCR)Purification


Posted on June 22, 2020
What is a cloning vector in molecular biology?
DNADNA and RNANucleic AcidsPolymerase Chain Reaction (PCR)RNA


Posted on June 22, 2020
What are the essential features of a plasmid cloning vector?
DNADNA and RNANucleic AcidsPolymerase Chain Reaction (PCR)RNA


Posted on June 22, 2020
Why are plasmids used as vectors in genetic engineering?
DNADNA and RNANucleic AcidsPolymerase Chain Reaction (PCR)RNA


Posted on June 22, 2020
What is horizontal gene transfer?
Cellular ProcessesConjugationDNADNA and RNANucleic AcidsPolymerase Chain Reaction (PCR)Transformation


Posted on June 22, 2020
What is cloned DNA used for?
Cellular ProcessesDNADNA and RNANucleic AcidsPolymerase Chain Reaction (PCR)


Posted on June 22, 2020
What is the difference between cloning and expression vectors?
Cellular ProcessesDNADNA and RNANucleic AcidsPolymerase Chain Reaction (PCR)RNA


Posted on June 22, 2020
Why does PCR reaction require a primer?
DNADNA and RNAPCRPCR primerPolymerase Chain Reaction (PCR)Polymerases


Posted on June 22, 2020
Why are 2 primers needed for PCR?
DNADNA and RNAPCRPCR primerPolymerase Chain Reaction (PCR)


Posted on June 22, 2020
Should PCR primers be complementary to each other?
DNADNA and RNAPCRPCR primerPolymerase Chain Reaction (PCR)Polymerases


Posted on June 22, 2020
What is a sense strand (or coding strand)?
DNADNA and RNANucleic AcidsPCRPolymerase Chain Reaction (PCR)dsDNAssDNA


Posted on June 22, 2020
How do I choose primers for PCR?
PCRPCR primerPolymerase Chain Reaction (PCR)


Posted on June 22, 2020
Why should primers used in PCR have similar melting temperatures?
DNAPCRPCR primerPolymerase Chain Reaction (PCR)ssDNA


Posted on June 22, 2020
What is the difference between melting temperature and annealing temperature?
DNAPCRPolymerase Chain Reaction (PCR)ssDNA


Posted on June 22, 2020
What are the advantages of Taq polymerase in PCR?
DNADNA and RNA QuantitationPCRPolymerase Chain Reaction (PCR)PolymerasesReal-Time PCR (qPCR)


Posted on June 22, 2020
What are the limitations of Taq polymerase?
DNADNA and RNA QuantitationEnzymesPCRPolymerase Chain Reaction (PCR)PolymerasesReal-Time PCR (qPCR)


Posted on June 22, 2020
Are there different types of polymerases used in PCR?
DNADNA and RNA QuantitationEnzymesPCRPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)


Posted on June 22, 2020
What are the three stages of a PRC process?
DNADNA and RNA QuantitationEnzymesPCRPCR stepsPolymerase Chain Reaction (PCR)PolymerasesReal-Time PCR (qPCR)


Posted on June 22, 2020
What are the limitations of PCR technology?
DNADNA and RNA QuantitationPCRPolymerase Chain Reaction (PCR)PolymerasesReal-Time PCR (qPCR)


Posted on June 22, 2020
How does SYBR Green work in qPCR?
DNADNA and RNA QuantitationPCRPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Types of PCR


Posted on June 22, 2020
How do hydrolysis probes work in qPCR? probes work?
DNADNA and RNA QuantitationPCRPolymerase Chain Reaction (PCR)PolymerasesReal-Time PCR (qPCR)Types of PCR


Posted on June 22, 2020
What are the differences between the detection methods based on a fluorescent probe and an intercalating dye in qPCR?
DNADNA and RNA QuantitationPCRPeptide and OligonucleotidePolymerase Chain Reaction (PCR)PolymerasesReal-Time PCR (qPCR)


Posted on June 22, 2020
What is the melting curve analysis in qPCR?
DNADNA and RNA QuantitationPCRPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)qPCR


Posted on June 22, 2020
Are there different types of fluorescent probes used in qPCR?
Peptide and Oligonucleotide LabelingDNA and RNA QuantitationDNA and RNAPCRPhysiological ProbesPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)


Posted on May 9, 2020
What is next-generation sequencing (NGS)?
Chemical ReagentsDNA and RNADNA sequencingPhysiological ProbesPolymerase Chain Reaction (PCR)gene sequencing


Posted on May 9, 2020
What is a next-generation sequencing (NGS) library?
DNA and RNADNA sequencingPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)gene sequencingqPCR


Posted on May 9, 2020
How pure does DNA have to be for next-generation sequencing (NGS)?
DNA and RNADNA sequencingPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)gene sequencing


Posted on May 9, 2020
How does next-generation sequencing (NGS) work?
DNA and RNADNA sequencingPCRPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)gene sequencing


Posted on May 9, 2020
What is targeted next-generation sequencing (NGS)?
DNA and RNADNA sequencingNucleic AcidsPolymerase Chain Reaction (PCR)dna sequencing


Posted on May 9, 2020
How long does next-generation sequencing (NGS) take?
DNA and RNADNA sequencingNucleic AcidsPCRPolymerase Chain Reaction (PCR)gene sequencing


Posted on May 9, 2020
What is the difference between next-generation (NGS) and high-throughput sequencing (HTS)?
DNA and RNADNA sequencingNucleic AcidsPCRPolymerase Chain Reaction (PCR)gene sequencing


Posted on May 9, 2020
Is next-generation sequencing (NGS) better than Sanger sequencing?
DNA and RNA QuantitationDNA and RNADNA sequencingNucleic AcidsPolymerase Chain Reaction (PCR)gene sequencing


Posted on May 9, 2020
Can next-generation sequencing (NGS) be used for virus genomes?
DNA and RNA QuantitationDNA and RNANucleic AcidsPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)RNA


Posted on May 9, 2020
What is the function of DMSO in PCR?
Chemical ReagentsDMSOPCRPolymerase Chain Reaction (PCR)


Posted on May 9, 2020
What is the function of MgCl2 in PCR?
Chemical ReagentsDNAPCRPhysiological ProbesPolymerase Chain Reaction (PCR)Polymerasesmagnesium


Posted on May 9, 2020
Why am I getting multiple bands for my PCR results?
DNA and RNADNA sequencingPCRPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)gene sequencing


Posted on May 9, 2020
For primer dilution in PCR, should I use water or TE buffer?
Chemical ReagentsBuffer Preparations and RecipesPCRPolymerase Chain Reaction (PCR)buffer


Posted on May 9, 2020
What are PCR primers?
Antibodies and ProteomicsDNA and RNAPCRPCR primerPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)qPCR


Posted on April 24, 2020
What is end-point RT-PCR?
Chemical ReagentsClassic DyesDNA and RNA QuantitationDNA and RNAFluorescencePCRPhysiological ProbesPolymerase Chain Reaction (PCR)RNAReverse Transcription PCR (RT-PCR)ethidium bromide


Posted on April 17, 2020
What is the difference between uses of Agarose gel electrophoresis and Polyacrylamide gel electrophoresis?
Agarose gel electrophoresisDNA and RNA QuantitationDNA sequencingGel ElectrophoresisPolymerase Chain Reaction (PCR)polyacrylamide gelMacromoleculeNucleic AcidsPhysiological Probes


Posted on April 7, 2020
6-VIC, SE is a popular amino-reactive fluorescent probe that is widely used in nucleic acid sequencing and related research. Is it also often used for labeling oligos?
Peptide and Oligonucleotide LabelingDNA sequencingPCRPhysiological ProbesPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on April 2, 2020
What is Sanger sequencing and what is it used for?
DNA sequencingGel ElectrophoresisPCRPolymerase Chain Reaction (PCR)electrophoresisDNA and RNA QuantitationDNA and RNAReverse Transcription PCR (RT-PCR)