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AAT Bioquest

Posted on May 30, 2023
What is the significance of unicellular organisms?
Bioconjugation Chemical ReagentsCellular Structures and OrganellesNucleusPhysiological Probesbacteria


Posted on May 30, 2023
What are the examples of unicellular organisms?
Bioconjugation Chemical ReagentsCellular Structures and OrganellesNucleusPhysiological Probesbacteria


Posted on May 30, 2023
What are the types of unicellular organisms?
Bioconjugation Chemical ReagentsCellular Structures and OrganellesNucleusPhysiological Probesbacteria


Posted on May 10, 2023
What are the characteristics of dextran?
Bioconjugation Chemical Reagentsdextranlive cell tracking


Posted on May 10, 2023
What are the advantages of fluorophore-labeled dextran?
Bioconjugation Chemical Reagentsdextranlive cell analysislive cell tracking


Posted on May 10, 2023
What are the applications of dextran conjugates?
Bioconjugation Chemical Reagentsdextranlive cell analysislive cell tracking


Posted on May 10, 2023
What are the degrees of labeling of the dextran conjugates?
Bioconjugation Chemical Reagentsdextranlive cell analysislive cell tracking


Posted on May 10, 2023
What are the general properties of FITC-dextran?
Bioconjugation Chemical Reagentsdextranlive cell analysislive cell tracking


Posted on May 10, 2023
What are the general properties of TRITC-dextran?
Bioconjugation Chemical Reagentsdextranlive cell analysislive cell tracking


Posted on May 10, 2023
What factors affect the stability of dextran?
Bioconjugation Chemical Reagentsdextranlive cell analysislive cell tracking


Posted on May 10, 2023
How do I dissolve dextran?
Bioconjugation Chemical Reagentsdextranlive cell analysislive cell tracking


Posted on May 10, 2023
What are labeled dextran conjugates used for?
Bioconjugation Chemical Reagentsdextranlive cell analysislive cell tracking


Posted on May 10, 2023
How are dextran conjugates purified?
Bioconjugation Chemical ReagentsPurificationdextranlive cell analysislive cell tracking


Posted on May 10, 2023
What are the factors that can affect TRITC-dextran conjugation?
Bioconjugation Chemical Reagentsdextranlive cell analysislive cell tracking


Posted on May 10, 2023
What is TRITC-dextran conjugate used for?
Bioconjugation Chemical Reagentsdextranlive cell analysislive cell tracking


Posted on May 10, 2023
What are the factors that can affect dextran-FITC conjugation?
Bioconjugation Chemical Reagentsdextranlive cell analysislive cell tracking


Posted on May 10, 2023
What are the applications of dextran-FITC conjugation?
Bioconjugation Chemical ReagentsFlow Cytometry ReagentsMicroscopySpectral Flow Cytometrydextranlive cell analysislive cell tracking


Posted on May 9, 2023
Is the hydrolysis of ATP to ADP reversible?
BioconjugationADPATPCell MetabolismCell Proliferation AssaysCell SignalingCellular ProcessesEnzymes


Posted on May 9, 2023
Is ATP a nucleotide?
BioconjugationADPATPCell MetabolismCell Proliferation AssaysCell SignalingCellular ProcessesEnzymesPhysiological Probes


Posted on May 9, 2023
What makes ATP relatively unstable?
BioconjugationADPATPCell MetabolismCell Proliferation AssaysCell SignalingCellular ProcessesEnzymesPhysiological Probes


Posted on May 9, 2023
Does hydrolysis release energy?
BioconjugationADPATPCell MetabolismCell Proliferation AssaysCell SignalingCellular ProcessesEnergyEnzymesPhysiological Probes


Posted on May 9, 2023
Is ATP hydrolysis reversible?
BioconjugationADPATPCell MetabolismCell Proliferation AssaysCell SignalingCellular ProcessesEnergyEnzymesPhysiological Probes


Posted on May 9, 2023
Is ATP hydrolysis exergonic?
BioconjugationADPATPCell MetabolismCell Proliferation AssaysCell SignalingCellular ProcessesEnergyEnzymesPhysiological Probes


Posted on May 9, 2023
How do I detect intracellular ATP levels?
BioconjugationADPATPCell Proliferation AssaysCell SignalingCellular ProcessesEnergyEnzymesFluorescencePhysiological Probesluminescense


Posted on May 9, 2023
What advantages does FACS have over MACS?
BioconjugationCellular ProcessesClassic DyesFlow Cytometry ReagentsSpectral Flow Cytometry


Posted on May 9, 2023
How do I choose the best cell separation method for my research?
BioconjugationCellular ProcessesClassic DyesFlow Cytometry ReagentsSpectral Flow Cytometry


Posted on May 9, 2023
What are the key measures of performance for cell separation methods?
Bioconjugation Chemical ReagentsFlow Cytometry ReagentsSpectral Flow Cytometry


Posted on May 9, 2023
What are the relationships between FACS and flow cytometry?
BioconjugationChemical ReagentsFlow Cytometry ReagentsFluorescenceSpectral Flow Cytometry


Posted on May 9, 2023
What are the applications of flow cytometry and FACS?
BioconjugationApoptosisCell Cycle AnalysisCell Proliferation AssaysChemical ReagentsFlow Cytometry ReagentsFluorescenceSpectral Flow Cytometry


Posted on May 9, 2023
How does cell sorting work?
Bioconjugation Chemical ReagentsFlow Cytometry ReagentsFluorescenceSpectral Flow Cytometry


Posted on May 9, 2023
What are the differences between magnetic-activated cell sorting and FACS?
Bioconjugation Chemical ReagentsFlow Cytometry ReagentsFluorescenceSpectral Flow Cytometry


Posted on May 9, 2023
What are the pros of MACS?
Bioconjugation Chemical ReagentsFlow Cytometry ReagentsFluorescenceSpectral Flow Cytometry


Posted on May 9, 2023
What are the mechanisms used to label cells in MACS?
Bioconjugation Chemical ReagentsFlow Cytometry ReagentsFluorescenceSpectral Flow Cytometry


Posted on May 2, 2023
What are the two ways to label DNA molecules?
BioconjugationCellular Structures and OrganellesChemical ReagentsDNADNA and RNA QuantitationNucleic AcidsNucleusPhysiological Probes


Posted on April 18, 2023
What are the steps involved in labeling an antibody using an antibody labeling kit?
Antibodies and Proteomics BioconjugationAntibody LabelingAntibody and Protein Labeling


Posted on April 18, 2023
What should I consider when I select antibodies?
Antibodies and Proteomics BioconjugationAntibody LabelingAntibody and Protein Labeling


Posted on April 18, 2023
How do I choose the appropriate antibody labeling kit?
Antibodies and Proteomics BioconjugationAntibody LabelingAntibody and Protein Labeling


Posted on April 18, 2023
What types of antibodies can be labeled using antibody labeling kits?
Antibodies and Proteomics Bioconjugation Enzyme-Linked Immunosorbent Assay (ELISA)Antibody LabelingAntibody and Protein LabelingImmunohistochemistry (IHC)Spectral Flow Cytometry


Posted on April 18, 2023
What types of labels can be attached to antibodies?
Antibodies and Proteomics Bioconjugation Enzyme-Linked Immunosorbent Assay (ELISA)Antibody LabelingAntibody and Protein LabelingImmunohistochemistry (IHC)Western Blot


Posted on April 18, 2023
How does an antibody labeling kit work?
Antibodies and Proteomics BioconjugationAntibody LabelingAntibody and Protein Labeling


Posted on April 18, 2023
What is the purpose of an antibody labeling kit?
Antibodies and Proteomics BioconjugationAntibody LabelingAntibody and Protein LabelingImmunohistochemistry (IHC)Spectral Flow Cytometry


Posted on April 18, 2023
How do I choose a method for protein purification?
Antibodies and Proteomics BioconjugationAffinity ChromatographyAntibody LabelingAntibody and Protein LabelingChromatographic techniquesProteinProtein Purification


Posted on April 18, 2023
What are the main types of chromatography techniques?
Antibodies and Proteomics BioconjugationAffinity ChromatographyAntibody LabelingAntibody and Protein LabelingChromatographic techniques


Posted on April 13, 2023
What are the types of apoptotic signaling pathways?
BioconjugationApoptosisApoptosis and NecrosisCell Viability AssaysCellular Processes


Posted on April 13, 2023
What types of samples can be used with an antifade mount medium?
BioconjugationFluorescenceImmunohistochemistry (IHC)anti-fadingfluorescence brightnessfluorescence microscope


Posted on April 13, 2023
How should an antifade reagent be chosen for a specific experiment or analysis?
BioconjugationFluorescenceImmunohistochemistry (IHC)anti-fadingfluorescence brightnessfluorescence microscope


Posted on April 13, 2023
Can antifade reagents be used in other types of imaging besides fluorescence microscopy?
BioconjugationFluorescenceImmunohistochemistry (IHC)anti-fadingfluorescence brightnessfluorescence microscope


Posted on April 13, 2023
How long does the fluorescence signal last when using an antifade mount medium?
BioconjugationFluorescenceImmunohistochemistry (IHC)anti-fadingfluorescence brightnessfluorescence microscope


Posted on April 13, 2023
How do I reduce sample exposure in order to minimize photobleaching?
BioconjugationFluorescenceImmunohistochemistry (IHC)anti-fadingfluorescence brightnessfluorescence microscope


Posted on April 13, 2023
What are some common anti-fading techniques?
BioconjugationFluorescenceImmunohistochemistry (IHC)anti-fadingfluorescence brightnessfluorescence microscope


Posted on April 13, 2023
What are some common components of an antifade mounting medium?
BioconjugationFluorescenceImmunohistochemistry (IHC)anti-fadingfluorescence brightnessfluorescence microscope


Posted on April 13, 2023
What causes fading in fluorescence microscopy?
BioconjugationFluorescenceImmunohistochemistry (IHC)anti-fadingfluorescence brightnessfluorescence microscope


Posted on April 13, 2023
Can antifade mounting medium be used with all types of fluorophores?
BioconjugationFluorescenceImmunohistochemistry (IHC)anti-fadingfluorescence brightness


Posted on April 12, 2023
What are the similarities between hormones and enzymes?
BioconjugationCellular MetabolismCellular ProcessesCellular Structures and OrganellesEnzymesPhysiological Probes


Posted on April 12, 2023
What are the differences between hormones and enzymes?
BioconjugationCellular MetabolismCellular ProcessesCellular Structures and OrganellesEnzymesPhysiological Probes


Posted on April 12, 2023
What are the examples of irreversible inhibitors?
BioconjugationCellular MetabolismCellular ProcessesCellular Structures and OrganellesEnzymesPhysiological Probes


Posted on April 12, 2023
What are the similarities between reversible and irreversible enzyme inhibition?
BioconjugationCellular MetabolismCellular ProcessesCellular Structures and OrganellesEnzymesPhysiological Probes


Posted on April 12, 2023
What is the significance of extracellular enzymes?
BioconjugationCellular MetabolismCellular ProcessesCellular Structures and OrganellesEnzymesPhysiological Probes


Posted on April 12, 2023
What are the differences between noncompetitive inhibition and uncompetitive inhibition?
BioconjugationCellular MetabolismCellular ProcessesCellular Structures and OrganellesEnzymesPhysiological Probes


Posted on April 12, 2023
What are the similarities between intracellular and extracellular enzymes?
BioconjugationCellular MetabolismCellular ProcessesCellular Structures and OrganellesEnzymesPhysiological Probes


Posted on April 12, 2023
How does temperature affect enzyme activities?
BioconjugationCellular MetabolismCellular ProcessesCellular Structures and OrganellesEnzymesPhysiological Probes


Posted on April 12, 2023
What are the differences between intracellular enzymes and extracellular enzymes?
BioconjugationCellular MetabolismCellular ProcessesCellular Structures and OrganellesEnzymesPhysiological Probes


Posted on February 9, 2023
What are the characteristics of gram-positive bacteria?
BioconjugationCellular Structures and OrganellesGram-positivebacteria


Posted on February 9, 2023
How do bacteria reproduce?
BioconjugationCellular Structures and OrganellesDNA and RNAbacteria


Posted on February 9, 2023
What is the smallest cell organelle in a cell?
BioconjugationCellular Structures and OrganellesNucleic AcidsProtein


Posted on February 9, 2023
How are plasmids passed on to other bacteria?
BioconjugationCellular Structures and Organellesbacteria


Posted on February 9, 2023
What are the three types of cell surface appendages of bacteria?
BioconjugationCellular Structures and Organellesbacteria


Posted on February 9, 2023
Do bacterial cells have DNA?
BioconjugationCellular Structures and OrganellesDNA and RNA


Posted on February 9, 2023
What are the similarities between bacteria and eukaryotes?
BioconjugationCellular Structures and Organellesbacteria


Posted on February 9, 2023
What are the differences between bacteria and eukaryotes?
BioconjugationCellular Structures and OrganellesDNA and RNAbacteria


Posted on February 9, 2023
What are the differences between archaea and bacteria?
BioconjugationCellular Structures and OrganellesGram Stainingbacteria


Posted on February 2, 2023
What is the structure of ribosomes?
BioconjugationCellular Structures and OrganellesDNA and RNA Quantitation


Posted on February 2, 2023
Why are ribosomes often referred to as the cell factories?
BioconjugationCellular Structures and OrganellesDNA and RNA Quantitation


Posted on February 2, 2023
Where are ribosomes made?
BioconjugationCellular Structures and OrganellesDNA and RNA Quantitation


Posted on February 2, 2023
What does "S" refer to in a 70s ribosome and 80s ribosome?
BioconjugationCellular Structures and OrganellesDNA and RNA Quantitation


Posted on February 2, 2023
What are the similarities between Shine Dalgarno and Kozak Sequence?
BioconjugationCellular Structures and OrganellesDNA and RNA Quantitation


Posted on February 2, 2023
What is the significance of the shine dalgarno sequence?
BioconjugationCellular Structures and OrganellesDNA and RNA Quantitation


Posted on January 30, 2023
What are the properties of glycine?
Bioconjugation Chemical ReagentsClassic DyesPolymerase Chain Reaction (PCR)


Posted on January 30, 2023
What are the types of luminescence?
Bioconjugation Enzyme-Linked Immunosorbent Assay (ELISA)Antibody and Protein LabelingImmunohistochemistry (IHC)


Posted on January 27, 2023
Is glycine a weak acid?
Bioconjugation Chemical ReagentsClassic DyesPolymerase Chain Reaction (PCR)


Posted on January 26, 2023
How do I solve the dead cell issue?
Antibodies and Proteomics BioconjugationCell Viability AssaysFlow Cytometry ReagentsLive or DeadSpectral Flow Cytometry


Posted on January 26, 2023
Why are dead cells problematic in flow cytometry?
Antibodies and Proteomics BioconjugationCell Viability AssaysCellular ProcessesDNA and RNALive or DeadSpectral Flow Cytometry


Posted on January 26, 2023
What do viruses and bacteria have in common?
Antibodies and Proteomics BioconjugationCellular Structures and OrganellesEnzymes


Posted on January 26, 2023
How are viruses and cells similar?
Antibodies and Proteomics BioconjugationCellular Structures and OrganellesDNA and RNAEnzymes


Posted on January 26, 2023
What are the similarities between virus and virion?
Antibodies and Proteomics BioconjugationCellular Structures and OrganellesDNA and RNAEnzymesProtein


Posted on January 26, 2023
What are the differences between virus and virion?
Antibodies and Proteomics BioconjugationCellular Structures and OrganellesDNA and RNANucleic Acids


Posted on January 26, 2023
What are the types of viruses based on the type of host?
Antibodies and Proteomics BioconjugationCellular Structures and OrganellesDNA and RNAEnzymesbacteria


Posted on January 26, 2023
Are viruses considered living?
Antibodies and Proteomics BioconjugationCellular Structures and OrganellesDNA and RNAEnzymesProtein


Posted on January 26, 2023
What are the basic components of virions?
Antibodies and Proteomics BioconjugationCellular Structures and OrganellesDNA and RNAEnzymesNucleic AcidsNucleusProtein


Posted on January 26, 2023
How are viruses classified?
Antibodies and Proteomics BioconjugationCellular Structures and OrganellesDNA and RNAEnzymesNucleic Acidsbacteria


Posted on January 26, 2023
What are the properties of a virus?
Antibodies and Proteomics BioconjugationEnzymesNucleic AcidsNucleusProteinbacteria


Posted on January 26, 2023
What are the steps of viral replication?
Antibodies and Proteomics BioconjugationCellular ProcessesDNA and RNAEnzymesNucleic AcidsProteinbacteria


Posted on January 26, 2023
What are the differences between lytic and lysogenic?
Antibodies and Proteomics BioconjugationDNA and RNAEnzymesNucleic AcidsProtein


Posted on January 26, 2023
What happens in the lysogenic cycle?
Antibodies and Proteomics BioconjugationDNA and RNAEnzymesNucleic AcidsProteinbacteria


Posted on January 26, 2023
What happens during lysogenic conversion?
Antibodies and Proteomics BioconjugationDNA and RNAEnzymesProteinbacteria


Posted on January 26, 2023
What are the differences between DNA viruses and RNA viruses?
Antibodies and Proteomics BioconjugationDNA and RNAEnzymesNucleic AcidsNucleusProtein


Posted on January 26, 2023
What is Baltimore classification?
Antibodies and Proteomics BioconjugationDNA and RNAEnzymesProtein


Posted on January 25, 2023
How do I choose the best method to assess cell survival?
BioconjugationCell Proliferation AssaysCell Viability AssaysCellular Processes


Posted on January 23, 2023
How do I mutate plasmids?
BioconjugationEnzymesNucleic AcidsPolymerase Chain Reaction (PCR)


Posted on January 20, 2023
What are the functions of plasmids?
BioconjugationCellular ProcessesCellular Structures and OrganellesDNA and RNANucleic AcidsNucleus


Posted on January 20, 2023
What are the common types of plasmids?
BioconjugationCellular ProcessesCellular Structures and OrganellesDNA and RNA QuantitationDNA and RNANucleic AcidsNucleus


Posted on January 20, 2023
What are the properties of a plasmid?
BioconjugationCellular ProcessesCellular Structures and OrganellesDNA and RNA QuantitationDNA and RNANucleic AcidsNucleus


Posted on January 20, 2023
What should I do if I get very few, if any, transformants when transforming large plasmids ((>10 kb) or BACS?
BioconjugationCellular ProcessesCellular Structures and OrganellesDNA and RNANucleic AcidsNucleus


Posted on January 20, 2023
What are the methods for plasmid detection?
BioconjugationCellular ProcessesCellular Structures and OrganellesDNA and RNA QuantitationDNA and RNANucleic AcidsNucleus


Posted on January 20, 2023
How do I increase plasmid yield?
BioconjugationCellular ProcessesCellular Structures and OrganellesDNA and RNA QuantitationDNA and RNA


Posted on January 20, 2023
How is plasmid removed from DNA?
BioconjugationCellular ProcessesCellular Structures and OrganellesDNA and RNA QuantitationDNA and RNANucleic AcidsNucleus


Posted on January 18, 2023
How do I dissolve BSA powder?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingBSAImmunohistochemistry (IHC)bovine serum albumin


Posted on January 13, 2023
What are the differences between bioluminescence and fluorescence?
Bioconjugation Chemical ReagentsEnzymesFluorescenceLuciferasefluorescence imagingluminescense


Posted on January 13, 2023
What are the types of molecules that exhibit fluorescence?
Bioconjugation Chemical ReagentsCoumarinsCyaninesFluoresceinsFluorescenceRhodamines and Rhodamine Derivativesfluorescence imaging


Posted on January 13, 2023
What are the different types of spectrometers used in fluorometry?
Bioconjugation Chemical ReagentsFluorescenceemission wavelengthexcitation wavelengthfluorescence imagingspectrophotometer


Posted on January 13, 2023
What are the differences between static quenching and dynamic quenching?
Bioconjugation Chemical Reagents Förster Resonance Energy Transfer (FRET)Classic DyesPhysiological ProbesPolymerase Chain Reaction (PCR)Tide Quencher Dyes


Posted on January 13, 2023
What are the applications of fluorescence quenching?
Bioconjugation Chemical Reagents Förster Resonance Energy Transfer (FRET)Classic DyesDNADNA and RNA QuantitationFluorescencePhysiological Probes


Posted on January 13, 2023
What is the mechanism of dynamic quenching?
Bioconjugation Chemical Reagents Förster Resonance Energy Transfer (FRET)Classic DyesFluorescencePhysiological ProbesPolymerase Chain Reaction (PCR)Tide Quencher Dyes


Posted on January 13, 2023
What are the types of fluorescence quenching?
Bioconjugation Chemical Reagents Förster Resonance Energy Transfer (FRET)Classic DyesFluorescencePhysiological ProbesPolymerase Chain Reaction (PCR)Tide Quencher Dyes


Posted on January 12, 2023
What are the factors that cause fluorescence quenching?
Bioconjugation Förster Resonance Energy Transfer (FRET)Chemical ReagentsFluorescencePhysiological ProbesTide Quencher Dyes


Posted on January 7, 2023
What is the purpose of active transport?
BioconjugationCell MetabolismCell Proliferation AssaysCell SignalingCellular Processes


Posted on January 7, 2023
What is the significance of active transport?
BioconjugationCell MetabolismCell Proliferation AssaysCell SignalingCellular Processes


Posted on January 7, 2023
Where are MMPs expressed?
BioconjugationAmplite® Reagents and Assay KitsEnzymesProteases


Posted on January 6, 2023
What are activation mechanisms of matrix metalloproteinases (MMPs)?
BioconjugationAmplite® Reagents and Assay KitsEnzymesProteases


Posted on January 6, 2023
How can MMPs be activated?
BioconjugationAmplite® Reagents and Assay KitsEnzymesProteases


Posted on January 6, 2023
What is the composition of an extracellular matrix?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCellular Structures and OrganellesMMPProteincollagen


Posted on January 6, 2023
Which MMPs are collagenases?
Antibodies and Proteomics Bioconjugation Förster Resonance Energy Transfer (FRET)Antibody and Protein LabelingEnzymesMMPProtein


Posted on January 6, 2023
What are the MMPs enzymes in cells?
Antibodies and Proteomics Bioconjugation Chemical Reagents Förster Resonance Energy Transfer (FRET)Antibody and Protein LabelingEnzymesMMPProtein


Posted on January 6, 2023
What are the functions of proteoglycans?
Antibodies and Proteomics Bioconjugation Chemical ReagentsAntibody and Protein LabelingCell SignalingPhysiological ProbesProtein


Posted on January 6, 2023
What is the structure of proteoglycans?
Antibodies and Proteomics Bioconjugation Chemical ReagentsAntibody and Protein LabelingProtein


Posted on January 6, 2023
What are the types of structural proteins in the extracellular matrix and their functions?
Antibodies and Proteomics Bioconjugation Chemical ReagentsAntibody and Protein LabelingCellular Structures and OrganellesPhysiological Probes


Posted on January 6, 2023
What is the extracellular matrix?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCellular Structures and Organelles


Posted on January 6, 2023
What are the differences between eukaryotic DNA and prokaryotic DNA?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCellular Structures and OrganellesDNAImmunohistochemistry (IHC)Nucleic AcidsNucleus


Posted on December 14, 2022
Can atoms be subdivided?
BioconjugationChemical Reagents


Posted on December 13, 2022
What are the differences between atoms and molecules?
Bioconjugation Chemical ReagentsCell Metabolism


Posted on December 13, 2022
What are the examples of an atom?
Bioconjugation Chemical ReagentsCell Metabolism


Posted on December 13, 2022
Can a molecule have only one atom?
Bioconjugation Chemical ReagentsCell Metabolism


Posted on December 13, 2022
What is a simple molecule?
Bioconjugation Chemical ReagentsCell Metabolism


Posted on December 13, 2022
How do atoms become molecules?
Bioconjugation Chemical ReagentsCell Metabolism


Posted on December 13, 2022
Why can't we see an atom with the naked eye?
Bioconjugation Chemical ReagentsCell Metabolism


Posted on December 13, 2022
What are the limitations of Dalton's atomic theory?
Bioconjugation Chemical ReagentsCell Metabolism


Posted on December 13, 2022
What are the general principles of Dalton's atomic theory?
Bioconjugation Chemical ReagentsCell Metabolism


Posted on December 13, 2022
What is the basis for Dalton’s Atomic Theory?
Bioconjugation Chemical ReagentsCell Metabolism


Posted on December 13, 2022
What are atoms made of?
Bioconjugation Chemical ReagentsCell Metabolism


Posted on December 13, 2022
What is the size of an atom?
Bioconjugation Chemical ReagentsCell Metabolism


Posted on December 13, 2022
Is salt a molecule?
Bioconjugation Chemical ReagentsCell Metabolism


Posted on December 13, 2022
Is oxygen a molecule?
Bioconjugation Chemical ReagentsCell Metabolism


Posted on December 10, 2022
Does DAPI stain all cells?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCell Cycle AssaysCellular ProcessesCellular Structures and OrganellesDAPIImmunohistochemistry (IHC)Nucleus


Posted on December 10, 2022
Can DAPI bind to RNA?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCell Cycle AssaysCellular ProcessesCellular Structures and OrganellesDAPIImmunohistochemistry (IHC)Nucleus


Posted on December 10, 2022
Do I have to fix cells before DAPI staining?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCell Cycle AssaysCellular ProcessesCellular Structures and OrganellesDAPIImmunohistochemistry (IHC)Nucleus


Posted on December 2, 2022
Is DAPI toxic to cells?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCell Cycle AssaysCellular Structures and OrganellesDAPIImmunohistochemistry (IHC)Nucleus


Posted on December 2, 2022
What is DAPI a marker of?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCellular Structures and OrganellesDAPIImmunohistochemistry (IHC)Nucleus


Posted on December 2, 2022
What organelle does DAPI stain?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCell Cycle AssaysCellular ProcessesCellular Structures and OrganellesDAPIImmunohistochemistry (IHC)Nucleus


Posted on December 2, 2022
Why is DAPI used as a counterstain?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCell Cycle AssaysCellular ProcessesCellular Structures and OrganellesDAPIImmunohistochemistry (IHC)Nucleus


Posted on December 2, 2022
What should I consider when choosing counterstain?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCell Cycle AssaysCellular ProcessesCellular ProcesssesDAPIImmunohistochemistry (IHC)Nucleus


Posted on December 2, 2022
What procedures may be required to prepare a sample for cellular staining?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCell Cycle AssaysCellular ProcessesCellular Structures and OrganellesDAPIImmunohistochemistry (IHC)Nucleus


Posted on December 2, 2022
What are the common cell stains?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCell Cycle AssaysCellular ProcessesCellular Structures and OrganellesDAPIImmunohistochemistry (IHC)


Posted on December 2, 2022
What laser excites DAPI?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCell Cycle AssaysCellular ProcessesCellular Structures and OrganellesDAPIImmunohistochemistry (IHC)Nucleus


Posted on November 30, 2022
What do I dissolve DAPI in?
BioconjugationAntibody and Protein LabelingCell Structures and OrganellesImmunohistochemistry (IHC)Nucleus


Posted on November 23, 2022
What cells express CD3?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCluster of Differentiation (CD Antibodies)Immunohistochemistry (IHC)


Posted on November 23, 2022
What is CD3 a marker of?
BioconjugationAntibodies and ProteomicsAntibody and Protein LabelingCluster of Differentiation (CD Antibodies)Immunohistochemistry (IHC)


Posted on November 23, 2022
What is the structure of CD19?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCluster of Differentiation (CD Antibodies)Immunohistochemistry (IHC)


Posted on November 23, 2022
What are the functions of CD19?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCluster of Differentiation (CD Antibodies)Immunohistochemistry (IHC)


Posted on November 23, 2022
What is CD19?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCluster of Differentiation (CD Antibodies)Immunohistochemistry (IHC)


Posted on November 23, 2022
What are the functions of CD14?
BioconjugationAntibodies and ProteomicsAntibody and Protein LabelingCluster of Differentiation (CD Antibodies)Immunohistochemistry (IHC)


Posted on November 23, 2022
What cells express CD34?
BioconjugationAntibodies and ProteomicsAntibody and Protein LabelingCluster of Differentiation (CD Antibodies)Immunohistochemistry (IHC)


Posted on November 23, 2022
What is the structure of CD34?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCluster of Differentiation (CD Antibodies)Immunohistochemistry (IHC)


Posted on November 23, 2022
What is CD34?
BioconjugationAntibodies and ProteomicsAntibody and Protein LabelingCluster of Differentiation (CD Antibodies)Immunohistochemistry (IHC)


Posted on November 23, 2022
What is CD34 a marker for?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCluster of Differentiation (CD Antibodies)Immunohistochemistry (IHC)


Posted on November 23, 2022
Is Foxp3 a Treg marker?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCluster of Differentiation (CD Antibodies)


Posted on November 22, 2022
What cells express Foxp3?
BioconjugationAntibody and Protein LabelingAntibody and ProteomicsCluster of Differentiation (CD Antibodies)


Posted on November 22, 2022
Where are Treg cells produced?
BioconjugationAntibody and Protein LabelingAntibody and ProteomicsCluster of Differentiation (CD Antibodies)


Posted on November 22, 2022
What is the significance of a Treg?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCluster of Differentiation (CD Antibodies)


Posted on November 22, 2022
What are the commonly used CD makers for the analysis of Tregs by flow cytometry?
BioconjugationAntibody and Protein LabelingCluster of Differentiation (CD Antibodies)


Posted on November 21, 2022
What organelles does a plant cell have but an animal cell doesn't?
Bioconjugation Chemical ReagentsCellular ProcessesCellular Structures and OrganellesPhysiological Probes


Posted on November 21, 2022
What are the functions of intermediate filaments?
Bioconjugation Chemical ReagentsCellular ProcessesCellular Structures and Organelles


Posted on November 21, 2022
What are the functions of actin filaments?
Bioconjugation Chemical ReagentsActinCellular ProcessesCellular Structures and OrganellesMitochondriaPhysiological Probes


Posted on November 16, 2022
How does trypan blue determine cell viability?
BioconjugationCell Cycle AssaysCell Proliferation AssaysCell Viability AssaysCellular Processes


Posted on November 16, 2022
How does trypan blue distinguish living and dead cells?
BioconjugationCell Proliferation AssaysCell ViabilityCell Viability AssaysCellular Processes


Posted on November 16, 2022
What does MTT measure?
BioconjugationCell CytotoxicityCell Proliferation AssaysCell Viability AssaysCellular Processes


Posted on November 16, 2022
What are the main functions of the cell membrane?
BioconjugationCell Structures and OrganellesMitochondriaPlasma Membrane


Posted on November 16, 2022
How does MTT assay determine cell viability?
BioconjugationCell Proliferation AssaysCell Viability AssaysCellular Processes


Posted on November 15, 2022
What are the similarities between luciferase and GFP?
BioconjugationAmplite® Reagents and Assay KitsEnzymesLuciferases


Posted on November 14, 2022
What are the similarities between Fluorophore and Chromophore?
Bioconjugation Chemical Reagents Förster Resonance Energy Transfer (FRET)DNA and RNA QuantitationDyes by Functional GroupPCRPhysiological ProbesPolymerase Chain Reaction (PCR)


Posted on November 14, 2022
What is the difference between Black Hole Quencher (BHQ) dye and TAMRA?
Bioconjugation Chemical ReagentsClassic DyesDNA and RNA QuantitationFluorescencePCRPhysiological ProbesPolymerase Chain Reaction (PCR)


Posted on November 14, 2022
What are the differences between GFP and YFP?
Antibodies and Proteomics Bioconjugation Chemical ReagentsAntibody and Protein LabelingClassic DyesGreen FluorescenceProtein


Posted on November 14, 2022
What activates GFP?
Antibodies and Proteomics Bioconjugation Chemical ReagentsAntibody and Protein LabelingClassic DyesGreen FluorescenceProtein


Posted on November 14, 2022
What are the applications of GFP?
Antibodies and Proteomics Bioconjugation Chemical ReagentsAntibody and Protein LabelingChemical ReagentsClassic DyesGreen FluorescenceProtein


Posted on November 14, 2022
What's the relationship between GFP and EGFP?
Antibodies and Proteomics Bioconjugation Chemical ReagentsAntibody and Protein LabelingClassic DyesGreen FluorescenceProtein


Posted on November 14, 2022
What are the differences between GFP and EGFP?
Antibodies and Proteomics Bioconjugation Chemical ReagentsAntibody and Protein LabelingClassic DyesGreen FluorescenceProtein


Posted on November 14, 2022
What are the types of fluorophores?
Antibodies and Proteomics Bioconjugation Chemical ReagentsClassic DyesFluoresceinsProteinRhodamines and Rhodamine Derivatives


Posted on November 10, 2022
What are the applications of TRITC-dextran?
Antibodies and Proteomics Bioconjugation Chemical ReagentsAntibody and Protein LabelingClassic DyesFlow Cytometry ReagentsFluoresceinsImmunohistochemistry (IHC)Spectral Flow Cytometryamine-reactiveimmunocytochemistry


Posted on November 10, 2022
How are FITC signals detected?
Antibodies and Proteomics Bioconjugation Chemical ReagentsAntibody and Protein LabelingClassic DyesFluoresceinsImmunohistochemistry (IHC)amine-reactiveimmunocytochemistry


Posted on November 10, 2022
How do I store FITC-dextran?
Antibodies and Proteomics Bioconjugation Chemical ReagentsAntibody and Protein LabelingClassic DyesFlow Cytometry ReagentsFluoresceinsImmunohistochemistry (IHC)Spectral Flow Cytometryamine-reactiveimmunocytochemistry


Posted on November 10, 2022
What are the different isomers of FITC?
Antibodies and Proteomics Bioconjugation Chemical ReagentsAntibody and Protein LabelingClassic DyesFlow Cytometry ReagentsFluoresceinsImmunohistochemistry (IHC)Spectral Flow Cytometryamine-reactiveimmunocytochemistry


Posted on November 10, 2022
What are the physical properties of FITC?
Antibodies and Proteomics Bioconjugation Chemical ReagentsAntibody and Protein LabelingClassic DyesFlow Cytometry ReagentsFluoresceinsImmunohistochemistry (IHC)Spectral Flow Cytometryimmunocytochemistry


Posted on November 10, 2022
How do I dissolve a FITC-dextran?
Bioconjugation Chemical ReagentsClassic DyesFlow Cytometry ReagentsFluoresceinsImmunohistochemistry (IHC)Spectral Flow Cytometryamine-reactiveimmunocytochemistry


Posted on November 10, 2022
Is FITC hydrophobic?
Antibodies and Proteomics Bioconjugation Chemical ReagentsAntibody and Protein LabelingClassic DyesFlow Cytometry ReagentsFluoresceinsImmunohistochemistry (IHC)Spectral Flow Cytometryimmunocytochemistry


Posted on November 10, 2022
Is FITC a fluorophore?
Antibodies and Proteomics Bioconjugation Chemical ReagentsAntibody and Protein LabelingClassic DyesFlow Cytometry ReagentsFluoresceinsImmunohistochemistry (IHC)Spectral Flow Cytometryamine-reactiveimmunocytochemistry


Posted on November 1, 2022
What are the functions of actin based thin filaments?
Bioconjugation Chemical ReagentsActinCell Structures and OrganellesMitochondria


Posted on November 1, 2022
Where does polymerization of actin occur?
BioconjugationActinCell Structures and OrganellesMitochondriaPlasma Membrane


Posted on October 26, 2022
Does propidium iodide stain apoptotic cells?
BioconjugationApoptosis and NecrosisCell Cycle AssaysCell Structures and OrganellesCell Viability AssaysNucleus


Posted on October 25, 2022
What are the types of signaling molecules?
BioconjugationApoptosis and NecrosisCell SignalingCellular ProcessesG-Protein-Coupled Receptors (GPCR)Intracellular Ions


Posted on October 25, 2022
What are the steps involved in western blot?
Bioconjugation Enzyme-Linked Immunosorbent Assay (ELISA)Antibody and Protein LabelingHorseradish Peroxidase (HRP) and Poly-HRPSecondary AntibodiesWestern Blot


Posted on October 18, 2022
How are the membrane covering of mitochondria and lysosomes different from each other?
BioconjugationCell Structures and OrganellesLysosomesMitochondria


Posted on October 14, 2022
Are coumarin dyes pH sensitive?
Bioconjugation Chemical ReagentsClassic DyesCoumarins


Posted on October 14, 2022
What color do coumarins fluoresce?
Bioconjugation Chemical ReagentsClassic DyesCoumarins


Posted on October 14, 2022
What are the applications of coumarin dyes?
Bioconjugation Chemical ReagentsClassic DyesCoumarins


Posted on October 14, 2022
What does 'coumarin nucleus' mean?
Bioconjugation Chemical ReagentsClassic DyesCoumarins


Posted on October 14, 2022
What are the steps of IR spectroscopy/vibrational spectroscopy?
BioconjugationAntibody and Protein LabelingAntibody and ProteomicsClassic Dyes


Posted on September 28, 2022
What are the differences between PBS and HBSS?
BioconjugationTools


Posted on September 1, 2022
What is flow cytometry used for?
BioconjugationCell Cycle AssaysCell Viability AssaysCellular ProcessesFlow Cytometry Reagents


Posted on September 1, 2022
What are the differences between flow cytometry and FACS?
BioconjugationCell Structures and OrganellesCellular ProcessesClassic DyesFlow Cytometry Reagents


Posted on September 1, 2022
What are the Similarities Between Flow Cytometry and FACS?
BioconjugationCell Structures a nd OrganellesCellular ProcessesFlow Cytometry Reagents


Posted on September 1, 2022
What are the similarities between reporter genes and selectable markers?
BioconjugationAmplite® Reagents and Assay KitsEnzymesLuciferases


Posted on August 31, 2022
What is the difference between polymerization and crosslink?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingProtein to Protein Conjugation


Posted on August 31, 2022
Is formaldehyde more toxic than glutaraldehyde?
BioconjugationAmplite® Reagents and Assay KitsEnzymes


Posted on August 31, 2022
What are the applications of crosslinking chemistry?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingProtein to Protein Conjugation


Posted on August 31, 2022
What are the different types of crosslinking reagents?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingProtein to Protein Conjugation


Posted on August 31, 2022
What are the factors to consider when choosing a crosslinking reagent?
BioconjugationAntibody and Protein LabelingFlow Cytometry ReagentsProtein to Protein Conjugation


Posted on August 30, 2022
What are the principles of magnetic resonance spectroscopy?
Antibodies and Proteomics Bioconjugation Chemical ReagentsClassic Dyesspectrophotometer


Posted on August 30, 2022
What are the steps of magnetic resonance spectroscopy?
Antibodies and Proteomics Bioconjugation Chemical ReagentsClassic Dyesspectrophotometer


Posted on August 30, 2022
What are the uses of magnetic resonance spectroscopy?
Antibodies and Proteomics Bioconjugation Chemical Reagentsspectrophotometer


Posted on August 29, 2022
What are the similarities between immunoglobulin and antibody?
BioconjugationAntibodies and ProteomicsAntibody and Protein Labeling


Posted on August 29, 2022
What are the subclasses of immunoglobulins?
Antibodies and Proteomics BioconjugationAntibody and Protein Labeling


Posted on August 29, 2022
What are the differences between immunoglobulin and antibody?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingBioconjugationImmunohistochemistry (IHC)


Posted on August 29, 2022
What is the structure of immunoglobulins?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingImmunohistochemistry (IHC)


Posted on August 26, 2022
Do eukaryotic cells possess a plasma membrane?
BioconjugationCell Structures and OrganellesPlasma Membrane


Posted on August 23, 2022
What are the most common cell viability assays?
BioconjugationCell Proliferation AssaysCell Viability AssaysCellular Processes


Posted on August 23, 2022
What are the common methods used to measure cell proliferation?
BioconjugationCell Proliferation AssaysCell Viability AssaysCellular ProcessesCellular processes


Posted on August 23, 2022
How do I measure cell cytotoxicity?
BioconjugationCell CytotoxicityCell Proliferation AssaysCell Viability AssaysEnzymes


Posted on August 23, 2022
How do I measure cell proliferation?
BioconjugationCell Proliferation AssaysCell Viability AssaysCellular ProcessesDNA and RNA Quantitation


Posted on August 23, 2022
What is gram stain used for?
BioconjugationCell Viability AssaysCellular ProcessesGram Staining


Posted on August 23, 2022
How does gram stain work?
BioconjugationCell Viability AssaysCellular ProcessesGram Staining


Posted on August 23, 2022
What are the steps of gram staining?
BioconjugationCell Viability AssaysCellular ProcessesGram Staining


Posted on August 23, 2022
What is the primary stain for gram staining?
BioconjugationCell Viability AssaysCellular ProcessesGram Staining


Posted on August 19, 2022
What is the principle of IR spectroscopy/vibrational spectroscopy?
BioconjugationAntibody and Protein LabelingClassic Dyes


Posted on August 19, 2022
What are the uses of fluorescence spectroscopy?
BioconjugationAntibody and Protein LabelingClassic DyesFluorescence


Posted on August 19, 2022
What are the uses of IR spectroscopy/vibrational spectroscopy?
BioconjugationAntibody and Protein LabelingClassic Dyes


Posted on August 19, 2022
What are the principles of mass spectroscopy?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingClassic Dyes


Posted on August 19, 2022
What are the steps of mass spectroscopy?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingClassic Dyes


Posted on June 1, 2022
Why is BSA used as a standard?
Antibody and Protein Labeling Enzyme-Linked Immunosorbent Assay (ELISA)Immunohistochemistry (IHC) Antibodies and Proteomics Bioconjugation


Posted on November 14, 2020
How do you purify oligonucleotide conjugates by HPLC?
Bioconjugation Chemical Reagents Peptide and Oligonucleotide LabelingConjugationPeptide and Oligonucleotide


Posted on July 22, 2020
How does DAPI work?
Antibodies and Proteomics Bioconjugation Chemical ReagentsCell ViabilityCell Viability AssaysCellular ProcessesDAPI


Posted on June 1, 2020
Why streptavidin is better than avidin for binding biotin?
Antibodies and Proteomics Bioconjugation Enzyme-Linked Immunosorbent Assay (ELISA)BiotinBiotin and StreptavidinImmunohistochemistry (IHC)flow cytometryimmunofluorescence


Posted on June 1, 2020
What is bioconjugation?
BioconjugationConjugated proteinsDye to Peptide ConjugationProtein to Protein Conjugation


Posted on June 1, 2020
What is click chemistry?
BioconjugationConjugated proteinsDye to Peptide ConjugationProtein to Protein Conjugationantibody conjugation


Posted on June 1, 2020
What is bioorthogonal chemistry?
BioconjugationConjugated proteinsDye to Peptide ConjugationProtein to Protein Conjugationantibody conjugation


Posted on June 1, 2020
What is spillover in flow cytometry?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingFlow Cytometry ReagentsFluorescenceSpectral Flow Cytometryflow cytometry


Posted on June 1, 2020
What is fluorescence compensation in flow cytometry?
BioconjugationAntibody and Protein LabelingAntibody and ProteomicsFlow Cytometry ReagentsFluorescenceSpectral Flow Cytometryflow cytometry


Posted on June 1, 2020
What are tandem dyes in flow cytometry?
Bioconjugation Förster Resonance Energy Transfer (FRET)CyaninesFRETFlow Cytometry ReagentsPE and APCSpectral Flow Cytometryflow cytometry


Posted on June 1, 2020
What is the advantage of tandem dyes in flow cytometry?
Bioconjugation Chemical ReagentsClassic DyesFlow Cytometry ReagentsPE and APCSpectral Flow Cytometryflow cytometry


Posted on June 1, 2020
What are the types of ion-exchange resins?
Antibodies and Proteomics BioconjugationIntracellular Ions


Posted on May 18, 2020
Does Propidium Iodide specifically bind to dead cells?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCell Cycle AssaysCell Viability AssaysCellular Structures and OrganellesImmunohistochemistry (IHC)Propidium iodide (PI)


Posted on May 18, 2020
How do DNA binding dyes bind to DNA?
Antibodies and Proteomics Bioconjugation Chemical ReagentsAntibody and Protein LabelingCell Cycle AssaysCellular Structures and OrganellesDAPIImmunohistochemistry (IHC)Physiological ProbesPropidium iodide (PI)TUNEL


Posted on May 9, 2020
What phagocytic particles are best for assaying phagocytic activity of macrophages?
BioconjugationConjugationE. coliFluorescenceIntracellular pHPhysiological Probeslatex beadmultiplexphagocytosis


Posted on May 9, 2020
Which markers can be used to differentiate between human monocytes and macrophages?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCluster of Differentiation (CD Antibodies)Immunohistochemistry (IHC)flow cytometryimmunofluorescence


Posted on May 9, 2020
What markers can I use for investigating microglial activation in flow cytometry?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCluster of Differentiation (CD Antibodies)Flow Cytometry ReagentsIHCImmunohistochemistry (IHC)flow cytometry


Posted on May 9, 2020
What markers can I use for macrophages?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCluster of Differentiation (CD Antibodies)IHCImmunohistochemistry (IHC)


Posted on May 9, 2020
What markers can I use for neutrophils?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCluster of Differentiation (CD Antibodies)IHCImmunohistochemistry (IHC)


Posted on May 9, 2020
What markers can I use for plasma cells?
BioconjugationAntibody and Protein LabelingAntibody and ProteomicsCluster of Differentiation (CD Antibodies)IHCImmunohistochemistry (IHC)


Posted on May 9, 2020
What are the best markers to define mesenchymal stromal cell (MSC) phenotype by FACS?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCluster of Differentiation (CD Antibodies)Immunohistochemistry (IHC)


Posted on May 9, 2020
What are the activation markers of mouse dendritic cells?
Antibodies and Proteomics BioconjugationCluster of Differentiation (CD Antibodies)Immunohistochemistry (IHC)


Posted on May 9, 2020
Is there a spectral overlap between PerCP-Cy5.5 and PE-Cy5?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCluster of Differentiation (CD Antibodies)Flow Cytometry ReagentsImmunohistochemistry (IHC)PE and APCSpectral Flow Cytometry


Posted on May 9, 2020
Is mouse CD16/CD32 a marker for any particular cell type?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCluster of Differentiation (CD Antibodies)IHCImmunohistochemistry (IHC)


Posted on May 9, 2020
Can CD31 also be used as a marker for platelets instead of CD41?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCluster of Differentiation (CD Antibodies)IHCImmunohistochemistry (IHC)


Posted on May 9, 2020
How can one distinguish naive from effector/effector memory CD8 T cells?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCluster of Differentiation (CD Antibodies)IHCImmunohistochemistry (IHC)


Posted on May 9, 2020
Is it possible to use DAPI and PI simultaneously?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingDAPIFluorescenceImmunohistochemistry (IHC)Propidium iodide (PI)


Posted on April 24, 2020
Any suggestions on how to insert antibodies into living cells?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingImmunohistochemistry (IHC)


Posted on April 24, 2020
How does the structure of the variable domain of an antibody allow it to bind with high affinity to its epitope?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingImmunohistochemistry (IHC)


Posted on April 24, 2020
What is the difference between mycotoxins and bacterial toxins?
Antibodies and Proteomics BioconjugationProteinbacteria


Posted on April 24, 2020
Can polyclonal antibodies that are generated against tagged proteins cross-react?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingImmunohistochemistry (IHC)Protein


Posted on April 24, 2020
What are some methods for conjugation of antibodies with metallic nanoparticles?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingConjugationImmunohistochemistry (IHC)antibody conjugation


Posted on April 24, 2020
What is the difference between polyclonal and monoclonal antibodies?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCluster of Differentiation (CD Antibodies)IHCImmunohistochemistry (IHC)Protein


Posted on April 24, 2020
Why are there different antibody isotypes in our immune system?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingIHCIgGImmunohistochemistry (IHC)Physiological Probes


Posted on April 24, 2020
What is the difference between capture antibody and marker-antigen?
Antibodies and Proteomics Bioconjugation Enzyme-Linked Immunosorbent Assay (ELISA)Antibody and Protein LabelingELISAIHCImmunohistochemistry (IHC)


Posted on April 24, 2020
Why might polyclonal antibodies give better results than monoclonal antibodies?
Antibodies and Proteomics Bioconjugation Chemical ReagentsAntibody and Protein LabelingIHCImmunohistochemistry (IHC)Physiological Probes


Posted on April 24, 2020
What is the difference between recombinant and synthetic polypeptides?
Antibodies and Proteomics BioconjugationDNA and RNAPeptide and OligonucleotideProtein


Posted on April 24, 2020
What is the difference between silica gels and bare silica?
Antibodies and Proteomics Bioconjugation Chemical ReagentsAffinity ChromatographyChromatographic techniques


Posted on April 24, 2020
Can I use an HRP-conjugated primary antibody for western blot (with no secondary)?
Antibodies and Proteomics Bioconjugation Chemical ReagentsAntibody and Protein LabelingHRPHRP-labeled primary antibodyHorseradish Peroxidase (HRP) and Poly-HRPPrimary AntibodiesSecondary AntibodiesWestern Blotantibody conjugation


Posted on April 24, 2020
Why does a secondary antibody have to originate from a host species different than that of the primary antibody?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingImmunohistochemistry (IHC)Primary AntibodiesSecondary Antibodies


Posted on April 24, 2020
What is the difference between anti-human and anti-mouse antibodies?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCluster of Differentiation (CD Antibodies)Immunohistochemistry (IHC)antibody conjugation


Posted on April 24, 2020
Can I use unstained cells as negative controls for FACS analysis?
Antibodies and Proteomics BioconjugationPrimary Antibodiesantibody conjugation


Posted on April 24, 2020
What is the right DAPI concentration to use in a flow cytometry experiment?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCell Cycle AssaysCell Structure and OrganellesCellular ProcessesDAPIFlow Cytometry ReagentsSpectral Flow Cytometry


Posted on April 24, 2020
Can a human hormone assay kit be used for rats and mice?
Antibodies and Proteomics BioconjugationAntibody and Protein Labeling


Posted on April 24, 2020
What is the difference between HLA-DR and Iba1?
Antibodies and Proteomics BioconjugationIHCImmunohistochemistry (IHC)Protein


Posted on April 24, 2020
What is in antiserum?
Antibodies and Proteomics Bioconjugation Chemical ReagentsProtein


Posted on April 24, 2020
To generate antibodies in mice, should I use intraperitoneal or subcutaneous injection?
Antibodies and Proteomics BioconjugationAntibody and Protein Labeling


Posted on April 24, 2020
Why is TMB used in conjunction with HRP in ELISA?
Antibodies and Proteomics Bioconjugation Enzyme-Linked Immunosorbent Assay (ELISA)Antibody and Protein LabelingHRPHorseradish Peroxidase (HRP) and Poly-HRPImmunohistochemistry (IHC)


Posted on April 24, 2020
Is there a good antibody available to detect C-terminal fragments of amyloid precursor protein (APP)?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingIHCImmunohistochemistry (IHC)


Posted on April 24, 2020
What is the right concentration of antibodies to use when labelling with fluorescent dyes?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingImmunohistochemistry (IHC)


Posted on April 17, 2020
What is the function of adding BSA as buffer in Biotin-Streptavidin system?
Antibodies and Proteomics Bioconjugation Chemical ReagentsAntibody and Protein LabelingBSABiotin and StreptavidinBuffer Preparations and Recipesbovine serum albuminbuffer


Posted on April 17, 2020
What is the actual mechanism involved in blocking a nitrocelluolse membrane ?
Antibodies and Proteomics Bioconjugation Chemical ReagentsBSABuffer Preparations and Recipesbovine serum albuminbuffer


Posted on April 17, 2020
Does PBS buffer have any advantage over SSC buffer?
Bioconjugation Chemical ReagentsBuffer Preparations and RecipesDNA and RNAFISHPhysiological Probesbufferfluorescence microscopein situ hybridization


Posted on April 17, 2020
What is the detailed mechanism of PMA differentiation of U937 cells?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingIHCImmunohistochemistry (IHC)


Posted on April 17, 2020
Why is the function of E-Cadherin totally different from N-Cadherin?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingIHCImmunohistochemistry (IHC)


Posted on April 17, 2020
Which is the best conditions for methanol fixation of microtubules?
Bioconjugation Chemical ReagentsClassic DyesFixationPhysiological Probesfixed cells


Posted on April 17, 2020
How to quench glutaraldehyde fluorescence?
BioconjugationFluorescenceautofluorescence Antibodies and ProteomicsAntibody and Protein LabelingPhysiological Probes


Posted on April 17, 2020
Is Gelatin or BSA best for blocking in immunofluorescence?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingBSAimmunofluorescence Chemical Reagents


Posted on April 17, 2020
What's the role of BSA/Milk as blocking agents??
Antibodies and Proteomics Bioconjugation Enzyme-Linked Immunosorbent Assay (ELISA)Antibody and Protein LabelingBSABuffer Preparations and RecipesImmunohistochemistry (IHC)bovine serum albuminbuffer


Posted on April 17, 2020
What is the role of Tau protein aggregation in neurodegeneration?
Antibodies and Proteomics Bioconjugation Enzyme-Linked Immunosorbent Assay (ELISA)Antibody and Protein LabelingWestern Blot