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Posted on September 18, 2023
What are the types of streaking techniques in microbiology?
Antibodies and Proteomics BioconjugationAntibody and Protein Labeling

Posted on September 18, 2023
What are the functions of biomolecules?
Antibodies and Proteomics BioconjugationAntibody and Protein Labeling

Posted on September 18, 2023
What are the main classes of biomolecules?
Antibodies and Proteomics BioconjugationAntibody and Protein Labeling

Posted on September 7, 2023
What is the protein concentration of proteinase K (molecular biology grade)?
Antibodies and ProteomicsCalculating ConcentrationEnzymesProteinProtein Concentration Calculator

Posted on September 7, 2023
Why should I neutralize the proteinase K after incubation?
Antibodies and ProteomicsEnzymesProtein

Posted on September 7, 2023
What is the optimal pH for using proteinase K?
Antibodies and ProteomicsEnzymesProtein

Posted on September 7, 2023
What is the optimal temperature for activating proteinase K?
Antibodies and ProteomicsEnzymesProtein

Posted on September 7, 2023
Does EDTA inactivate proteinase K?
Antibodies and ProteomicsEDTAEnzymesProteasesProtein

Posted on September 7, 2023
What are the activators of proteinase K?
Antibodies and ProteomicsEnzymesProtein

Posted on September 7, 2023
How is proteinase K involved in cell lysis?
Antibodies and ProteomicsDNA and RNAEnzymesNucleic AcidsProteincell lysis

Posted on September 7, 2023
What is the relationship between proteinase K and calcium?
Antibodies and ProteomicsEnzymesProtein

Posted on September 7, 2023
How do I dissolve proteinase K?
Antibodies and ProteomicsEnzymesProtein

Posted on September 7, 2023
Is there an alternative to using proteinase K during DNA extractions?
Antibodies and ProteomicsDNA and RNADNA extractionEnzymesProtein

Posted on September 7, 2023
What are the differences between proteasome and protease?
Antibodies and ProteomicsEnzymesProteasesProtein

Posted on September 7, 2023
What are the similarities between proteasome and protease?
Antibodies and ProteomicsEnzymesProtein

Posted on September 7, 2023
What are the different types of proteases?
Antibodies and ProteomicsEnzymesProteasesProtein

Posted on September 7, 2023
What are the classifications of proteases?
Antibodies and ProteomicsEnzymesProteasesProtein

Posted on September 5, 2023
What are the functions of the skin?
Antibodies and ProteomicsImmunohistochemistry (IHC)Physiological Probes

Posted on September 5, 2023
What are the layers of skin?
Antibodies and ProteomicsImmunohistochemistry (IHC)Physiological Probes

Posted on September 5, 2023
What are the main cell types in the epidermis?
Antibodies and ProteomicsImmunohistochemistry (IHC)Physiological Probes

Posted on September 5, 2023
What are the differences between Epidermis and Dermis?
Antibodies and ProteomicsImmunohistochemistry (IHC)Physiological Probes

Posted on September 5, 2023
What are keratinocytes?
Antibodies and ProteomicsImmunohistochemistry (IHC)Physiological Probes

Posted on September 5, 2023
What are the functions of keratinocytes?
Antibodies and ProteomicsImmunohistochemistry (IHC)Physiological Probes

Posted on September 5, 2023
What is the relationship between keratinocytes and melanocytes?
Antibodies and ProteomicsImmunohistochemistry (IHC)Physiological Probes

Posted on September 5, 2023
What are the applications of keratinocytes?
Antibodies and ProteomicsImmunohistochemistry (IHC)Physiological Probes

Posted on September 5, 2023
What is the life cycle of the keratinocyte?
Antibodies and ProteomicsImmunohistochemistry (IHC)Physiological Probes

Posted on September 5, 2023
What are the types of keratinocytes?
Antibodies and ProteomicsImmunohistochemistry (IHC)Physiological Probes

Posted on September 5, 2023
What are melanocytes located?
Antibodies and ProteomicsImmunohistochemistry (IHC)Physiological Probes

Posted on September 5, 2023
What are the functions melanocytes?
Antibodies and ProteomicsImmunohistochemistry (IHC)Physiological Probes

Posted on September 5, 2023
What are the characteristics of melanocytes?
Antibodies and ProteomicsImmunohistochemistry (IHC)Physiological Probes

Posted on September 5, 2023
What is the life cycle of melanocytes?
Antibodies and ProteomicsImmunohistochemistry (IHC)Physiological Probes

Posted on September 5, 2023
What is the structure of melanocytes?
Antibodies and ProteomicsImmunohistochemistry (IHC)Physiological Probes

Posted on September 5, 2023
What are the functions of Merkel cells?
Antibodies and ProteomicsImmunohistochemistry (IHC)Physiological Probes

Posted on September 5, 2023
Where are Merkel cells located?
Antibodies and ProteomicsImmunohistochemistry (IHC)Physiological Probes

Posted on September 5, 2023
What are Langerhans cells?
Antibodies and ProteomicsImmunohistochemistry (IHC)Physiological Probes

Posted on September 5, 2023
What are the functions Langerhans cells?
Antibodies and ProteomicsImmunohistochemistry (IHC)Physiological Probes

Posted on September 5, 2023
What functional groups are found in proteins?
Antibodies and ProteomicsAntibody and Protein Labelingamino acid sequence

Posted on September 5, 2023
What are the types of protein-protein interactions?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingProtein to Protein Conjugation

Posted on September 5, 2023
What are the common methods used to analyze protein interactions?
Antibodies and ProteomicsAntibody and Protein LabelingBuccutite Crosslinkers and KitsCrosslinkersProtein to Protein Conjugation

Posted on September 5, 2023
What are the biological effects of protein-protein interactions?
Antibodies and ProteomicsAntibody and Protein LabelingBuccutite Crosslinkers and KitsCrosslinkers

Posted on August 29, 2023
What are the differences between antigen and antibody?
Antibodies and ProteomicsAntibody and Protein Labeling

Posted on August 29, 2023
What happens when an antigen enters your body?
Antibodies and ProteomicsAntibody and Protein Labeling

Posted on August 29, 2023
What are the types of antigens?
Antibodies and ProteomicsAntibody and Protein Labeling

Posted on August 29, 2023
Where are antibodies produced?
Antibodies and ProteomicsAntibody and Protein Labeling

Posted on August 24, 2023
What are the applications of T cells?
Antibodies and ProteomicsAntibody and Protein LabelingCD4+ T CellsCluster of Differentiation (CD Antibodies)

Posted on August 24, 2023
What are the similarities between T helper and T cytotoxic cells?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCD4+ T CellsCluster of Differentiation (CD Antibodies)

Posted on August 24, 2023
What are the differences between T helper and T cytotoxic cells?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCD4+ T CellsCluster of Differentiation (CD Antibodies)

Posted on August 24, 2023
What are the similarities between B cell receptor and T cell receptor?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCD4+ T CellsCluster of Differentiation (CD Antibodies)

Posted on August 24, 2023
What are the differences between B cell receptor and T cell receptor?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCD4+ T CellsCluster of Differentiation (CD Antibodies)

Posted on August 24, 2023
Why is the thymus important for T cells?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCD4+ T CellsCluster of Differentiation (CD Antibodies)

Posted on August 24, 2023
Are positive and negative selection unique to T cells?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCD4+ T CellsCluster of Differentiation (CD Antibodies)

Posted on August 24, 2023
Why is T cell selection important?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCD4+ T CellsCluster of Differentiation (CD Antibodies)

Posted on August 24, 2023
Can T cell selection go wrong?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCD4+ T CellsCluster of Differentiation (CD Antibodies)

Posted on August 24, 2023
How does positive selection impact T cell diversity?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCD4+ T CellsCluster of Differentiation (CD Antibodies)

Posted on August 24, 2023
How does negative selection promote self-tolerance?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCD4+ T CellsCluster of Differentiation (CD Antibodies)

Posted on August 24, 2023
Do positive and negative selection occur simultaneously?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCD4+ T CellsCluster of Differentiation (CD Antibodies)

Posted on August 24, 2023
What happens if T cells don't undergo T cell selection?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCD4+ T CellsCluster of Differentiation (CD Antibodies)

Posted on August 24, 2023
What are the stages of T cell maturation?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCD4+ T CellsCluster of Differentiation (CD Antibodies)

Posted on August 24, 2023
How are T-cells different from B-cells?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCD4+ T CellsCluster of Differentiation (CD Antibodies)

Posted on August 24, 2023
Where do CD8+ T cells originate?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCD4+ T CellsCluster of Differentiation (CD Antibodies)

Posted on August 24, 2023
How do cytotoxic T-cells and helper T-cells work together?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCD4+ T CellsCluster of Differentiation (CD Antibodies)

Posted on August 24, 2023
Are cytotoxic T-cells in innate immunity?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCD4+ T CellsCluster of Differentiation (CD Antibodies)

Posted on August 24, 2023
How are helper T-cells activated?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCD4+ T CellsCluster of Differentiation (CD Antibodies)

Posted on August 24, 2023
What do regulatory T-cells do?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCD4+ T CellsCluster of Differentiation (CD Antibodies)

Posted on August 24, 2023
What are the markers or surface molecules used to identify regulatory T-cells?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCD4+ T CellsCluster of Differentiation (CD Antibodies)

Posted on August 24, 2023
How are regulatory T cells different from other T cells?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCD4+ T CellsCluster of Differentiation (CD Antibodies)

Posted on August 24, 2023
What are the functions of suppressor T cells?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCD4+ T CellsCluster of Differentiation (CD Antibodies)

Posted on August 18, 2023
What are T-cells?
Antibodies and ProteomicsAntibody and Protein LabelingCD4+ T CellsCluster of Differentiation (CD Antibodies)

Posted on August 18, 2023
What are the different types of T-cells?
Antibodies and ProteomicsAntibody and Protein LabelingCD4+ T CellsCluster of Differentiation (CD Antibodies)

Posted on August 18, 2023
What is the function of T-cells?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCD4+ T CellsCluster of Differentiation (CD Antibodies)

Posted on August 18, 2023
How do T-cells work in the immune system?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCD4+ T CellsCluster of Differentiation (CD Antibodies)

Posted on August 18, 2023
Where are T-cells located?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCD4+ T CellsCluster of Differentiation (CD Antibodies)

Posted on August 18, 2023
What are the common conditions and disorders that affect T-cells?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCD4+ T CellsCluster of Differentiation (CD Antibodies)

Posted on August 18, 2023
What is the normal range of T-cells?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCD4+ T CellsCluster of Differentiation (CD Antibodies)

Posted on August 18, 2023
What does it mean if my T-cells are low?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCD4+ T CellsCluster of Differentiation (CD Antibodies)

Posted on August 18, 2023
What does it mean if my T-cells are high?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCD4+ T CellsCluster of Differentiation (CD Antibodies)

Posted on August 18, 2023
What are the common tests to check the health of my T-cells?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCD4+ T CellsCluster of Differentiation (CD Antibodies)

Posted on August 18, 2023
How can I boost my T-cells?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCD4+ T CellsCluster of Differentiation (CD Antibodies)

Posted on August 18, 2023
What are the differences between T-cells and antibodies?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCD4+ T CellsCluster of Differentiation (CD Antibodies)

Posted on August 18, 2023
Why are T-cells important?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCD4+ T CellsCluster of Differentiation (CD Antibodies)

Posted on August 18, 2023
How does cancer treatment affect T-cells?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCD4+ T CellsCluster of Differentiation (CD Antibodies)

Posted on August 18, 2023
When does T cell exhaustion occur?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCD4+ T CellsCluster of Differentiation (CD Antibodies)

Posted on August 18, 2023
What is the role of T cells in Covid-19 infection?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCD4+ T CellsCluster of Differentiation (CD Antibodies)

Posted on August 18, 2023
What are the similarities between positive and negative selection of T cells?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCD4+ T CellsCluster of Differentiation (CD Antibodies)

Posted on August 18, 2023
What are the differences between positive and negative selection of T cells?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCD4+ T CellsCluster of Differentiation (CD Antibodies)

Posted on August 18, 2023
What is the structure of a T cell receptor?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCD4+ T CellsCluster of Differentiation (CD Antibodies)

Posted on August 18, 2023
What is the process of T cell development?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCD4+ T CellsCluster of Differentiation (CD Antibodies)

Posted on August 18, 2023
What are the selection processes in thymus?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCD4+ T CellsCluster of Differentiation (CD Antibodies)

Posted on August 18, 2023
What are the similarities between helper T cells and regulatory T cells?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCD4+ T CellsCluster of Differentiation (CD Antibodies)

Posted on August 18, 2023
What is the difference between helper T cells and regulatory T cells?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCD4+ T CellsCluster of Differentiation (CD Antibodies)

Posted on August 18, 2023
What happens to T cells after positive and negative selection?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCD4+ T CellsCluster of Differentiation (CD Antibodies)

Posted on August 18, 2023
What are the types of regulatory T cells?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCD4+ T CellsCluster of Differentiation (CD Antibodies)

Posted on August 18, 2023
What are the most widely used molecular markers for Treg cells?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCluster of Differentiation (CD Antibodies)

Posted on August 18, 2023
What are the similarities between T cells and B cells?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCD4+ T CellsCluster of Differentiation (CD Antibodies)

Posted on August 18, 2023
What are the differences between T cells and B cells?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCD4+ T CellsCluster of Differentiation (CD Antibodies)

Posted on August 15, 2023
What are the advantages of cell-free protein expression system over the traditional in vivo systems?
Antibodies and Proteomics BioconjugationProtein

Posted on August 15, 2023
What are the limitations of cell-free protein expression system?
Antibodies and Proteomics BioconjugationProtein

Posted on August 10, 2023
How do I measure histone modification?
Antibodies and Proteomics Chemical ReagentsAntibody and Protein LabelingDNA and RNA QuantitationProtein

Posted on August 10, 2023
What are the advantages of native ChIP?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingDNA and RNA Quantitation

Posted on August 10, 2023
What are the disadvantages of Cross-linking ChIP?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingDNA and RNA Quantitation

Posted on August 7, 2023
What are the advantages of allophycocyanin (APC)?
Antibodies and ProteomicsAntibody and Protein LabelingCellular PorcessesFlow Cytometry ReagentsPE and APC

Posted on August 5, 2023
How are recombinant proteins made?
Antibodies and Proteomics BioconjugationDNA and RNAPolymerase Chain Reaction (PCR)ProteinTransfection

Posted on August 5, 2023
What are recombinant proteins used for?
Antibodies and Proteomics BioconjugationDNA and RNAProtein

Posted on August 5, 2023
What are the examples of recombinant proteins?
Antibodies and Proteomics BioconjugationDNA and RNAProtein

Posted on August 5, 2023
How does recombinant protein expression work?
Antibodies and Proteomics BioconjugationDNA and RNAProtein

Posted on August 5, 2023
Is insulin a recombinant protein?
Antibodies and Proteomics BioconjugationDNA and RNAProtein

Posted on August 5, 2023
What are the advantages of the mammalian protein expression system?
Antibodies and Proteomics BioconjugationDNA and RNAProtein

Posted on August 5, 2023
What are the limitations of the mammalian protein expression system?
Antibodies and Proteomics BioconjugationDNA and RNAProtein

Posted on August 5, 2023
What are the advantages of the bacterial protein expression system?
Antibodies and Proteomics BioconjugationDNA and RNAProtein

Posted on August 5, 2023
What is the limitation of the bacteria protein expression system?
Antibodies and Proteomics BioconjugationDNA and RNAProtein

Posted on August 5, 2023
What are the advantages of the insect protein expression system?
Antibodies and Proteomics BioconjugationDNA and RNAProtein

Posted on August 5, 2023
What are the advantages of the yeast protein expression system?
Antibodies and Proteomics BioconjugationDNA and RNAProtein

Posted on August 5, 2023
What are the limitations of the yeast protein expression system?
Antibodies and Proteomics BioconjugationDNA and RNAProtein

Posted on August 5, 2023
What are the applications of the mammalian protein expression system?
Antibodies and Proteomics BioconjugationDNA and RNAProtein

Posted on August 5, 2023
What are the applications of the bacterial protein expression system?
Antibodies and Proteomics BioconjugationDNA and RNAProtein

Posted on August 5, 2023
What are the applications of the insect protein expression system?
Antibodies and Proteomics BioconjugationDNA and RNAProtein

Posted on August 5, 2023
What are the applications of the yeast protein expression system?
Antibodies and Proteomics BioconjugationDNA and RNAProtein

Posted on August 5, 2023
What are the applications of cell-free protein expression systems?
Antibodies and Proteomics BioconjugationDNA and RNAProtein

Posted on August 5, 2023
What is the principle of cell-free protein expression systems?
Antibodies and Proteomics BioconjugationDNA and RNAProtein

Posted on August 5, 2023
What factors should I consider when choosing protein expression systems?
Antibodies and Proteomics BioconjugationDNA and RNAProtein

Posted on August 5, 2023
What are the pros of the baculovirus expression system?
Antibodies and Proteomics BioconjugationDNA and RNAProtein

Posted on August 5, 2023
When does protein expression occur?
Antibodies and Proteomics BioconjugationDNA and RNAProtein

Posted on August 5, 2023
What are the types of extracts used for cell-free protein expression?
Antibodies and Proteomics BioconjugationDNA and RNAProtein

Posted on August 4, 2023
What are the generally used detection methods in real time PCR (qPCR)?
Antibodies and ProteomicsAntibody and Protein LabelingDNA and RNA QuantitationPCR Detection of Viral DNA/RNAReal-Time PCR (qPCR)

Posted on August 4, 2023
What are the limitations of direct ELISA?
Antibodies and Proteomics Bioconjugation Enzyme-Linked Immunosorbent Assay (ELISA)Cell SignalingCellular Processes

Posted on August 4, 2023
What are the advantages of direct ELISA?
Antibodies and Proteomics Bioconjugation Enzyme-Linked Immunosorbent Assay (ELISA)Cell SignalingCellular Processes

Posted on August 3, 2023
What are the types and mechanisms of traditional vaccines?
Antibodies and ProteomicsDNA and RNA QuantitationNucleic AcidsNucleus

Posted on August 3, 2023
How is transcribed mRNA processed in eukaryotes?
Antibodies and ProteomicsDNA and RNA QuantitationNucleic AcidsNucleusPolymerase Chain Reaction (PCR)Reverse Transcription PCR (RT-PCR)

Posted on August 3, 2023
How is mRNA related to DNA?
Antibodies and ProteomicsDNA and RNA QuantitationEnzymesNucleic AcidsNucleus

Posted on August 3, 2023
What is the significance of mRNA degradation?
Antibodies and ProteomicsDNA and RNA QuantitationNucleic AcidsNucleus

Posted on August 3, 2023
What are the pathways of mRNA degradation?
Antibodies and ProteomicsDNA and RNA QuantitationNucleic AcidsNucleus

Posted on August 3, 2023
How are mRNA vaccines different from traditional vaccines?
Antibodies and ProteomicsDNA and RNA QuantitationNucleic AcidsNucleus

Posted on August 3, 2023
Can mRNA alter DNA?
Antibodies and ProteomicsDNA and RNA QuantitationNucleic AcidsNucleus

Posted on August 3, 2023
Besides vaccines, what other applications does mRNA have?
Antibodies and ProteomicsDNA and RNA QuantitationNucleic AcidsNucleus

Posted on August 3, 2023
What are the pros of using mRNA vaccines?
Antibodies and ProteomicsDNA and RNA QuantitationNucleic AcidsNucleus

Posted on August 3, 2023
What are the cons of using mRNA vaccines?
Antibodies and ProteomicsDNA and RNA QuantitationNucleic AcidsNucleus

Posted on August 3, 2023
What is the difference between an mRNA and a viral vector vaccine?
Antibodies and ProteomicsDNA and RNA QuantitationNucleic AcidsNucleus

Posted on August 3, 2023
What is the difference between a DNA and RNA vaccine?
Antibodies and ProteomicsDNA and RNA QuantitationNucleic AcidsNucleus

Posted on August 3, 2023
What are the pros of DNA vaccines?
Antibodies and ProteomicsDNA and RNA QuantitationNucleic AcidsNucleus

Posted on August 3, 2023
What are cons of DNA vaccines?
Antibodies and ProteomicsDNA and RNA QuantitationNucleic AcidsNucleus

Posted on August 3, 2023
What is the mechanism of DNA vaccines?
Antibodies and ProteomicsDNA and RNA QuantitationNucleic AcidsNucleus

Posted on August 3, 2023
What are the similarities between mRNA and DNA vaccines?
Antibodies and ProteomicsDNA and RNA QuantitationNucleic AcidsNucleus

Posted on August 3, 2023
What are the similarities between RNA and mRNA?
Antibodies and ProteomicsDNA and RNA QuantitationNucleic AcidsNucleus

Posted on August 3, 2023
What are the differences between RNA and mRNA?
Antibodies and ProteomicsDNA and RNA QuantitationNucleic AcidsNucleus

Posted on August 3, 2023
Why is the mRNA not equal in length to the DNA it was transcribed from?
Antibodies and ProteomicsDNA and RNA QuantitationNucleic AcidsNucleus

Posted on August 3, 2023
How does an mRNA get into the nuclear pore?
Antibodies and ProteomicsDNA and RNA QuantitationNucleic AcidsNucleus

Posted on July 28, 2023
What are the disadvantages of native ChIP?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingDNANucleic AcidsNucleusChromatin Immunoprecipitation (ChIP)Protein

Posted on July 28, 2023
What are the advantages of Cross-linking ChIP?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCellular Structures and OrganellesDNANucleic AcidsNucleusChromatin Immunoprecipitation (ChIP)Protein

Posted on July 28, 2023
How does ChIP work?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCellular Structures and OrganellesDNANucleic AcidsNucleusChromatin Immunoprecipitation (ChIP)Protein

Posted on July 28, 2023
What are the limitations of ChIP?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCellular Structures and OrganellesDNANucleic AcidsNucleusChromatin Immunoprecipitation (ChIP)Protein

Posted on July 28, 2023
What are the factors to consider when planning ChIP assays?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCellular Structures and OrganellesDNANucleic AcidsNucleusChromatin Immunoprecipitation (ChIP)Protein

Posted on July 25, 2023
What are the differences between cAMP and cGMP?
Antibodies and ProteomicsATPCell SignalingCellular ProcessesEnzymesPhysiological ProbesSecond Messenger

Posted on July 25, 2023
How is cGMP activated?
Antibodies and ProteomicsCell SignalingCellular ProcessesEnzymesPhysiological Probes

Posted on July 25, 2023
How is indocyanine green eliminated from the body?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCyaninesIndocyanine Green

Posted on July 21, 2023
What are the steps involved in a colorimetric direct ELISA?
Antibodies and Proteomics Enzyme-Linked Immunosorbent Assay (ELISA)Cell SignalingCellular Processes

Posted on June 27, 2023
What does ppm mean in mass spectrometry?
Antibodies and Proteomics Chemical ReagentsAntibody and Protein Labeling

Posted on June 27, 2023
What kind of radiation is used in mass spectroscopy?
Antibodies and Proteomics Chemical ReagentsAntibody and Protein Labeling

Posted on June 27, 2023
What is the base peak in mass spectrometry?
Antibodies and Proteomics Chemical ReagentsAntibody and Protein Labeling

Posted on June 27, 2023
What are the major components of a mass spectrometer?
Antibodies and Proteomics Chemical ReagentsAntibody and Protein Labeling

Posted on June 27, 2023
Who discovered mass spectroscopy?
Antibodies and Proteomics Chemical ReagentsAntibody and Protein Labeling

Posted on June 27, 2023
What does M/Z mean?
Antibodies and Proteomics Chemical ReagentsAntibody and Protein Labeling

Posted on June 27, 2023
How accurate is mass spectrometry?
Antibodies and Proteomics Chemical ReagentsAntibody and Protein Labeling

Posted on June 27, 2023
How does mass spectrometry identify proteins?
Antibodies and ProteomicsAntibody and Protein LabelingChemical ReagentsPhysiological Probes

Posted on June 21, 2023
What are the disadvantages of using mass spectrometry (MS) to detect phosphorylated proteins?
Antibodies and Proteomics Bioconjugation Chemical ReagentsAntibody and Protein LabelingPhysiological Probes

Posted on June 21, 2023
What are the advantages of using an antibody array to analyze phosphorylated proteins?
Antibodies and Proteomics Bioconjugation Chemical ReagentsAntibody and Protein LabelingPhysiological ProbesProtein

Posted on June 21, 2023
What are the disadvantages of using antibody arrays to analyze phosphorylated proteins?
Antibodies and Proteomics Bioconjugation Chemical ReagentsAntibody and Protein LabelingPhysiological ProbesProtein

Posted on June 15, 2023
What are the methods used to detect phosphorylated proteins?
Antibodies and ProteomicsEnzymesProteinWestern Blot

Posted on June 15, 2023
What are the major types of kinases?
Antibodies and ProteomicsCell Cycle AnalysisCell Cycle AssaysCell Cycle and ProliferationEnzymesProteinlipid

Posted on June 15, 2023
What are the differences between protein kinase A and protein kinase C?
Antibodies and ProteomicsCell SignalingCellular Structures and OrganellesEnzymesProtein

Posted on June 15, 2023
What are the similarities between protein kinase A and protein kinase C?
Antibodies and ProteomicsCell SignalingCellular ProcessesCellular Structures and OrganellesEnzymesProtein

Posted on May 24, 2023
What is the structure of protein kinases?
Antibodies and ProteomicsEnzymesProtein

Posted on May 24, 2023
How are protein kinases classified?
Antibodies and ProteomicsEnzymesProtein

Posted on May 24, 2023
What is the mechanism of phosphorylase reaction?
Antibodies and ProteomicsEnzymesProtein

Posted on May 24, 2023
How is phosphorylase regulated?
Antibodies and ProteomicsEnzymesProtein

Posted on May 24, 2023
Is protein phosphorylation reversible?
Antibodies and ProteomicsEnzymesProtein

Posted on May 24, 2023
What are the common methods available to detect kinase activities?
Antibodies and Proteomics Enzyme-Linked Immunosorbent Assay (ELISA) Förster Resonance Energy Transfer (FRET)EnzymesFluorescenceProteinReal-Time PCR (qPCR)qPCR

Posted on May 2, 2023
What is the significance of protein and DNA labeling?
Antibodies and ProteomicsAntibody and Protein LabelingDNA and RNANucleic AcidsNucleus

Posted on April 27, 2023
What are the differences between mRNA and tRNA?
Antibodies and ProteomicsDNA and RNARNATransfectionTransfection Reagents

Posted on April 27, 2023
What are the similarities between mRNA and tRNA?
Antibodies and ProteomicsDNA and RNARNATransfectionTransfection Reagents

Posted on April 27, 2023
What are the functions of mRNA?
Antibodies and ProteomicsDNA and RNANucleusRNATransfectionTransfection Reagents

Posted on April 27, 2023
Are mRNAs found in prokaryotes differ from those of eukaryotes?
Antibodies and ProteomicsDNA and RNANucleusRNATransfectionTransfection Reagents

Posted on April 27, 2023
What is the structure of mRNA?
Antibodies and ProteomicsDNA and RNARNATransfectionTransfection Reagents

Posted on April 27, 2023
What happens to mRNA after processing is complete?
Antibodies and ProteomicsDNA and RNARNATransfectionTransfection Reagents

Posted on April 18, 2023
What are the steps involved in labeling an antibody using an antibody labeling kit?
Antibodies and Proteomics BioconjugationAntibody LabelingAntibody and Protein Labeling

Posted on April 18, 2023
What should I consider when I select antibodies?
Antibodies and Proteomics BioconjugationAntibody LabelingAntibody and Protein Labeling

Posted on April 18, 2023
How do I choose the appropriate antibody labeling kit?
Antibodies and Proteomics BioconjugationAntibody LabelingAntibody and Protein Labeling

Posted on April 18, 2023
What types of antibodies can be labeled using antibody labeling kits?
Antibodies and Proteomics Bioconjugation Enzyme-Linked Immunosorbent Assay (ELISA)Antibody LabelingAntibody and Protein LabelingImmunohistochemistry (IHC)Spectral Flow Cytometry

Posted on April 18, 2023
What types of labels can be attached to antibodies?
Antibodies and Proteomics Bioconjugation Enzyme-Linked Immunosorbent Assay (ELISA)Antibody LabelingAntibody and Protein LabelingImmunohistochemistry (IHC)Western Blot

Posted on April 18, 2023
How does an antibody labeling kit work?
Antibodies and Proteomics BioconjugationAntibody LabelingAntibody and Protein Labeling

Posted on April 18, 2023
What is the purpose of an antibody labeling kit?
Antibodies and Proteomics BioconjugationAntibody LabelingAntibody and Protein LabelingImmunohistochemistry (IHC)Spectral Flow Cytometry

Posted on April 18, 2023
How do I choose a method for protein purification?
Antibodies and Proteomics BioconjugationAffinity ChromatographyAntibody LabelingAntibody and Protein LabelingChromatographic techniquesProteinProtein Purification

Posted on April 18, 2023
What are the main types of chromatography techniques?
Antibodies and Proteomics BioconjugationAffinity ChromatographyAntibody LabelingAntibody and Protein LabelingChromatographic techniques

Posted on March 27, 2023
What cellular structure is considered the worker of the cell and why?
Antibodies and ProteomicsCellular ProcessesCellular Structures and OrganellesProtein

Posted on March 21, 2023
What is the chemical mechanism by which the peptide bond is formed?
Antibodies and ProteomicsPeptide and OligonucleotideProteinamino acid sequence

Posted on March 21, 2023
What do peptide bonds hold together?
Antibodies and ProteomicsPeptide and OligonucleotideProteinamino acid sequence

Posted on March 21, 2023
What are the different forms of peptide bonds?
Antibodies and ProteomicsPeptide and OligonucleotideProteinamino acid sequence

Posted on March 21, 2023
How is peptide bond broken?
Antibodies and ProteomicsPeptide and OligonucleotideProteinamino acid sequence

Posted on March 21, 2023
Why is peptide bond planar?
Antibodies and ProteomicsPeptide and OligonucleotideProteinamino acid sequence

Posted on March 21, 2023
What are the chemical properties of peptides?
Antibodies and ProteomicsPeptide and OligonucleotideProteinamino acid sequence

Posted on March 21, 2023
What are the physical properties of peptides?
Antibodies and ProteomicsPeptide and OligonucleotideProteinamino acid sequence

Posted on March 21, 2023
What catalyzes the formation of peptide bonds?
Antibodies and ProteomicsEnzymesPeptide and OligonucleotideProteinamino acid sequence

Posted on March 21, 2023
What are the benefits of having peptide bonds?
Antibodies and ProteomicsPeptide and OligonucleotideProteinamino acid sequence

Posted on March 21, 2023
What are the characteristics of peptide bonds?
Antibodies and ProteomicsPeptide and OligonucleotideProteinamino acid sequence

Posted on February 7, 2023
What are the similarities between DNA methylation and histone acetylation?
Antibodies and ProteomicsCellular Structures and OrganellesDNA and RNANucleus

Posted on February 7, 2023
What are the differences between DNA methylation and histone acetylation?
Antibodies and ProteomicsCellular Structures and OrganellesDNA and RNANucleus

Posted on February 7, 2023
What are the types of histone modifications?
Antibodies and ProteomicsCellular Structures and OrganellesNucleus

Posted on February 7, 2023
What are the types of histones?
Antibodies and ProteomicsCellular Structures and OrganellesNucleus

Posted on January 26, 2023
How do I solve the dead cell issue?
Antibodies and Proteomics BioconjugationCell Viability AssaysFlow Cytometry ReagentsLive or DeadSpectral Flow Cytometry

Posted on January 26, 2023
Why are dead cells problematic in flow cytometry?
Antibodies and Proteomics BioconjugationCell Viability AssaysCellular ProcessesDNA and RNALive or DeadSpectral Flow Cytometry

Posted on January 26, 2023
What do viruses and bacteria have in common?
Antibodies and Proteomics BioconjugationCellular Structures and OrganellesEnzymes

Posted on January 26, 2023
How are viruses and cells similar?
Antibodies and Proteomics BioconjugationCellular Structures and OrganellesDNA and RNAEnzymes

Posted on January 26, 2023
What are the similarities between virus and virion?
Antibodies and Proteomics BioconjugationCellular Structures and OrganellesDNA and RNAEnzymesProtein

Posted on January 26, 2023
What are the differences between virus and virion?
Antibodies and Proteomics BioconjugationCellular Structures and OrganellesDNA and RNANucleic Acids

Posted on January 26, 2023
What are the types of viruses based on the type of host?
Antibodies and Proteomics BioconjugationCellular Structures and OrganellesDNA and RNAEnzymesbacteria

Posted on January 26, 2023
Are viruses considered living?
Antibodies and Proteomics BioconjugationCellular Structures and OrganellesDNA and RNAEnzymesProtein

Posted on January 26, 2023
What are the basic components of virions?
Antibodies and Proteomics BioconjugationCellular Structures and OrganellesDNA and RNAEnzymesNucleic AcidsNucleusProtein

Posted on January 26, 2023
How are viruses classified?
Antibodies and Proteomics BioconjugationCellular Structures and OrganellesDNA and RNAEnzymesNucleic Acidsbacteria

Posted on January 26, 2023
What are the properties of a virus?
Antibodies and Proteomics BioconjugationEnzymesNucleic AcidsNucleusProteinbacteria

Posted on January 26, 2023
What are the steps of viral replication?
Antibodies and Proteomics BioconjugationCellular ProcessesDNA and RNAEnzymesNucleic AcidsProteinbacteria

Posted on January 26, 2023
What are the differences between lytic and lysogenic?
Antibodies and Proteomics BioconjugationDNA and RNAEnzymesNucleic AcidsProtein

Posted on January 26, 2023
What happens in the lysogenic cycle?
Antibodies and Proteomics BioconjugationDNA and RNAEnzymesNucleic AcidsProteinbacteria

Posted on January 26, 2023
What happens during lysogenic conversion?
Antibodies and Proteomics BioconjugationDNA and RNAEnzymesProteinbacteria

Posted on January 26, 2023
What are the differences between DNA viruses and RNA viruses?
Antibodies and Proteomics BioconjugationDNA and RNAEnzymesNucleic AcidsNucleusProtein

Posted on January 26, 2023
What is Baltimore classification?
Antibodies and Proteomics BioconjugationDNA and RNAEnzymesProtein

Posted on January 18, 2023
How do I dissolve BSA powder?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingBSAImmunohistochemistry (IHC)bovine serum albumin

Posted on January 6, 2023
What is the composition of an extracellular matrix?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCellular Structures and OrganellesMMPProteincollagen

Posted on January 6, 2023
Which MMPs are collagenases?
Antibodies and Proteomics Bioconjugation Förster Resonance Energy Transfer (FRET)Antibody and Protein LabelingEnzymesMMPProtein

Posted on January 6, 2023
What are the MMPs enzymes in cells?
Antibodies and Proteomics Bioconjugation Chemical Reagents Förster Resonance Energy Transfer (FRET)Antibody and Protein LabelingEnzymesMMPProtein

Posted on January 6, 2023
What are the functions of proteoglycans?
Antibodies and Proteomics Bioconjugation Chemical ReagentsAntibody and Protein LabelingCell SignalingPhysiological ProbesProtein

Posted on January 6, 2023
What is the structure of proteoglycans?
Antibodies and Proteomics Bioconjugation Chemical ReagentsAntibody and Protein LabelingProtein

Posted on January 6, 2023
What are the types of structural proteins in the extracellular matrix and their functions?
Antibodies and Proteomics Bioconjugation Chemical ReagentsAntibody and Protein LabelingCellular Structures and OrganellesPhysiological Probes

Posted on January 6, 2023
What is the extracellular matrix?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCellular Structures and Organelles

Posted on January 6, 2023
What are the differences between eukaryotic DNA and prokaryotic DNA?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCellular Structures and OrganellesDNAImmunohistochemistry (IHC)Nucleic AcidsNucleus

Posted on December 10, 2022
Does DAPI stain all cells?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCell Cycle AssaysCellular ProcessesCellular Structures and OrganellesDAPIImmunohistochemistry (IHC)Nucleus

Posted on December 10, 2022
Can DAPI bind to RNA?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCell Cycle AssaysCellular ProcessesCellular Structures and OrganellesDAPIImmunohistochemistry (IHC)Nucleus

Posted on December 10, 2022
Do I have to fix cells before DAPI staining?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCell Cycle AssaysCellular ProcessesCellular Structures and OrganellesDAPIImmunohistochemistry (IHC)Nucleus

Posted on December 2, 2022
Is DAPI toxic to cells?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCell Cycle AssaysCellular Structures and OrganellesDAPIImmunohistochemistry (IHC)Nucleus

Posted on December 2, 2022
What is DAPI a marker of?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCellular Structures and OrganellesDAPIImmunohistochemistry (IHC)Nucleus

Posted on December 2, 2022
What organelle does DAPI stain?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCell Cycle AssaysCellular ProcessesCellular Structures and OrganellesDAPIImmunohistochemistry (IHC)Nucleus

Posted on December 2, 2022
Why is DAPI used as a counterstain?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCell Cycle AssaysCellular ProcessesCellular Structures and OrganellesDAPIImmunohistochemistry (IHC)Nucleus

Posted on December 2, 2022
What should I consider when choosing counterstain?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCell Cycle AssaysCellular ProcessesCellular ProcesssesDAPIImmunohistochemistry (IHC)Nucleus

Posted on December 2, 2022
What procedures may be required to prepare a sample for cellular staining?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCell Cycle AssaysCellular ProcessesCellular Structures and OrganellesDAPIImmunohistochemistry (IHC)Nucleus

Posted on December 2, 2022
What are the common cell stains?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCell Cycle AssaysCellular ProcessesCellular Structures and OrganellesDAPIImmunohistochemistry (IHC)

Posted on December 2, 2022
What laser excites DAPI?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCell Cycle AssaysCellular ProcessesCellular Structures and OrganellesDAPIImmunohistochemistry (IHC)Nucleus

Posted on November 23, 2022
What cells express CD3?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCluster of Differentiation (CD Antibodies)Immunohistochemistry (IHC)

Posted on November 23, 2022
What is the structure of CD19?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCluster of Differentiation (CD Antibodies)Immunohistochemistry (IHC)

Posted on November 23, 2022
What are the functions of CD19?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCluster of Differentiation (CD Antibodies)Immunohistochemistry (IHC)

Posted on November 23, 2022
What is CD19?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCluster of Differentiation (CD Antibodies)Immunohistochemistry (IHC)

Posted on November 23, 2022
What is the structure of CD34?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCluster of Differentiation (CD Antibodies)Immunohistochemistry (IHC)

Posted on November 23, 2022
What is CD34 a marker for?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCluster of Differentiation (CD Antibodies)Immunohistochemistry (IHC)

Posted on November 23, 2022
Is Foxp3 a Treg marker?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCluster of Differentiation (CD Antibodies)

Posted on November 22, 2022
What is the significance of a Treg?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCluster of Differentiation (CD Antibodies)

Posted on November 14, 2022
What are the differences between GFP and YFP?
Antibodies and Proteomics Bioconjugation Chemical ReagentsAntibody and Protein LabelingClassic DyesGreen FluorescenceProtein

Posted on November 14, 2022
What activates GFP?
Antibodies and Proteomics Bioconjugation Chemical ReagentsAntibody and Protein LabelingClassic DyesGreen FluorescenceProtein

Posted on November 14, 2022
What are the applications of GFP?
Antibodies and Proteomics Bioconjugation Chemical ReagentsAntibody and Protein LabelingChemical ReagentsClassic DyesGreen FluorescenceProtein

Posted on November 14, 2022
What's the relationship between GFP and EGFP?
Antibodies and Proteomics Bioconjugation Chemical ReagentsAntibody and Protein LabelingClassic DyesGreen FluorescenceProtein

Posted on November 14, 2022
What are the differences between GFP and EGFP?
Antibodies and Proteomics Bioconjugation Chemical ReagentsAntibody and Protein LabelingClassic DyesGreen FluorescenceProtein

Posted on November 14, 2022
What are the types of fluorophores?
Antibodies and Proteomics Bioconjugation Chemical ReagentsClassic DyesFluoresceinsProteinRhodamines and Rhodamine Derivatives

Posted on November 14, 2022
How is GFP produced?
Antibodies and ProteomicsGreen FluorescenceProteinSpectral Flow Cytometryflow cytometry

Posted on November 14, 2022
How is GFP detected?
Antibodies and ProteomicsGreen FluorescenceProteinSpectral Flow Cytometryflow cytometry

Posted on November 10, 2022
What are the applications of TRITC-dextran?
Antibodies and Proteomics Bioconjugation Chemical ReagentsAntibody and Protein LabelingClassic DyesFlow Cytometry ReagentsFluoresceinsImmunohistochemistry (IHC)Spectral Flow Cytometryamine-reactiveimmunocytochemistry

Posted on November 10, 2022
How are FITC signals detected?
Antibodies and Proteomics Bioconjugation Chemical ReagentsAntibody and Protein LabelingClassic DyesFluoresceinsImmunohistochemistry (IHC)amine-reactiveimmunocytochemistry

Posted on November 10, 2022
How do I store FITC-dextran?
Antibodies and Proteomics Bioconjugation Chemical ReagentsAntibody and Protein LabelingClassic DyesFlow Cytometry ReagentsFluoresceinsImmunohistochemistry (IHC)Spectral Flow Cytometryamine-reactiveimmunocytochemistry

Posted on November 10, 2022
What are the different isomers of FITC?
Antibodies and Proteomics Bioconjugation Chemical ReagentsAntibody and Protein LabelingClassic DyesFlow Cytometry ReagentsFluoresceinsImmunohistochemistry (IHC)Spectral Flow Cytometryamine-reactiveimmunocytochemistry

Posted on November 10, 2022
What are the physical properties of FITC?
Antibodies and Proteomics Bioconjugation Chemical ReagentsAntibody and Protein LabelingClassic DyesFlow Cytometry ReagentsFluoresceinsImmunohistochemistry (IHC)Spectral Flow Cytometryimmunocytochemistry

Posted on November 10, 2022
Is FITC hydrophobic?
Antibodies and Proteomics Bioconjugation Chemical ReagentsAntibody and Protein LabelingClassic DyesFlow Cytometry ReagentsFluoresceinsImmunohistochemistry (IHC)Spectral Flow Cytometryimmunocytochemistry

Posted on November 10, 2022
Is FITC soluble in water?
Antibodies and Proteomics Chemical ReagentsAntibody and Protein LabelingClassic DyesFlow Cytometry ReagentsFluoresceinsImmunohistochemistry (IHC)Spectral Flow Cytometryamine-reactiveimmunocytochemistry

Posted on November 10, 2022
Is FITC a fluorophore?
Antibodies and Proteomics Bioconjugation Chemical ReagentsAntibody and Protein LabelingClassic DyesFlow Cytometry ReagentsFluoresceinsImmunohistochemistry (IHC)Spectral Flow Cytometryamine-reactiveimmunocytochemistry

Posted on November 10, 2022
What does FITC bind to?
Antibodies and Proteomics Chemical ReagentsAntibody and Protein LabelingClassic DyesFlow Cytometry ReagentsFluoresceinsFluorescenceImmunohistochemistry (IHC)immunocytochemistryin situ hybridization

Posted on November 7, 2022
What are apoptotic signals?
Antibodies and ProteomicsApoptosis and NecrosisCell Viability AssaysCellular Processescell death

Posted on October 14, 2022
What are the common types of microscope light sources?
Antibodies and ProteomicsBioconjugationNucleusPhysiological Probes

Posted on October 7, 2022
What are the advantages of polyclonal antibodies?
Antibodies and ProteomicsAntibody and Protein LabelingBioconjugationImmunohistochemistry (IHC)

Posted on September 19, 2022
What does each peak in the chromatogram correspond to?
Antibodies and ProteomicsPhysiological Probes

Posted on August 31, 2022
What is the difference between polymerization and crosslink?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingProtein to Protein Conjugation

Posted on August 31, 2022
What are the applications of crosslinking chemistry?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingProtein to Protein Conjugation

Posted on August 31, 2022
What are the different types of crosslinking reagents?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingProtein to Protein Conjugation

Posted on August 30, 2022
How are epithelial tissues classified?
Antibodies and ProteomicsBioconjugationCell Structures and OrganellesCell Viability AssaysCellular Processes

Posted on August 30, 2022
What are the principles of magnetic resonance spectroscopy?
Antibodies and Proteomics Bioconjugation Chemical ReagentsClassic Dyesspectrophotometer

Posted on August 30, 2022
What are the steps of magnetic resonance spectroscopy?
Antibodies and Proteomics Bioconjugation Chemical ReagentsClassic Dyesspectrophotometer

Posted on August 30, 2022
What are the uses of magnetic resonance spectroscopy?
Antibodies and Proteomics Bioconjugation Chemical Reagentsspectrophotometer

Posted on August 29, 2022
What are the subclasses of immunoglobulins?
Antibodies and Proteomics BioconjugationAntibody and Protein Labeling

Posted on August 29, 2022
What are the differences between immunoglobulin and antibody?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingBioconjugationImmunohistochemistry (IHC)

Posted on August 29, 2022
What is the structure of immunoglobulins?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingImmunohistochemistry (IHC)

Posted on August 19, 2022
What are the principles of mass spectroscopy?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingClassic Dyes

Posted on August 19, 2022
What are the steps of mass spectroscopy?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingClassic Dyes

Posted on July 26, 2022
What is Cytokeratin 18?
Antibodies and Proteomics Enzyme-Linked Immunosorbent Assay (ELISA)Antibody LabelingImmunohistochemistry (IHC)Spectral Flow CytometryWestern Blotflow cytometryimmunocytochemistry

Posted on June 1, 2022
Why is BSA used as a standard?
Antibody and Protein Labeling Enzyme-Linked Immunosorbent Assay (ELISA)Immunohistochemistry (IHC) Antibodies and Proteomics Bioconjugation

Posted on September 29, 2020
What is the difference between Western Blot and ELISA?
Antibodies and Proteomics Chemical Reagents Enzyme-Linked Immunosorbent Assay (ELISA)Antibody and Protein LabelingImmunohistochemistry (IHC)Western Blot

Posted on September 17, 2020
How do I set up a Sandwich ELISA?
Antibodies and Proteomics Enzyme-Linked Immunosorbent Assay (ELISA)Antibody and Protein LabelingELISA

Posted on July 22, 2020
How can I dissociate adherent cells?
Antibodies and Proteomics Chemical ReagentsCell Cycle and ProliferationEnzymesadherent cells

Posted on July 22, 2020
How does DAPI work?
Antibodies and Proteomics Bioconjugation Chemical ReagentsCell ViabilityCell Viability AssaysCellular ProcessesDAPI

Posted on July 22, 2020
What are the different types of restriction enzymes?
Antibodies and ProteomicsCell Cycle and ProliferationCellular Structures and OrganellesEnzymesProtein

Posted on June 22, 2020
What are the types of introns?
Antibodies and ProteomicsDNADNA and RNAProteinRNA

Posted on June 22, 2020
What is a His tag? How does it work?
Antibodies and ProteomicsAntibody and Protein LabelingProteinProtein Purification

Posted on June 22, 2020
What are the 4 levels of protein structure?
Antibodies and ProteomicsPeptide and OligonucleotideProtein

Posted on June 22, 2020
What are the main interactions that stabilize each level of protein structure?
Antibodies and ProteomicsPeptide and OligonucleotideProtein

Posted on June 22, 2020
What is the relationship between protein structure and protein function?
Antibodies and ProteomicsPeptide and OligonucleotideProtein

Posted on June 22, 2020
What are the main protein types and their functions?
Antibodies and ProteomicsEnzymesPeptide and OligonucleotideProtein

Posted on June 22, 2020
What is protein denaturation?
Antibodies and ProteomicsPeptide and OligonucleotideProtein

Posted on June 22, 2020
How does denaturation affect the 4 structure levels of a protein?
Antibodies and ProteomicsProtein

Posted on June 22, 2020
What level of protein structure is not disturbed by denaturing?
Antibodies and ProteomicsPeptide and OligonucleotideProtein

Posted on June 22, 2020
How do acids and bases denature a protein?
Antibodies and ProteomicsPeptide and OligonucleotideProtein

Posted on June 22, 2020
How does heat denature a protein?
Antibodies and ProteomicsProtein

Posted on June 22, 2020
How does alcohol denature a protein?
Antibodies and Proteomics Chemical ReagentsProtein

Posted on June 22, 2020
How does (sodium dodecyl sulfate) SDS denature a protein?
Antibodies and ProteomicsProteindetergent

Posted on June 22, 2020
Is protein denaturation reversible?
Antibodies and ProteomicsProtein

Posted on June 22, 2020
What is the difference between protein degradation and denaturation?
Antibodies and ProteomicsPeptide and OligonucleotideProtein

Posted on June 1, 2020
What is the difference between nitrocellulose and PVDF membranes in western blot?
Antibodies and ProteomicsGel ElectrophoresisProtein PurificationWestern Blot

Posted on June 1, 2020
What is the difference between direct and indirect ELISA?
Antibodies and Proteomics Enzyme-Linked Immunosorbent Assay (ELISA)Antibody and Protein LabelingDirect ELISAPrimary AntibodiesSecondary Antibodies

Posted on June 1, 2020
What is the difference between direct and sandwich ELISA?
Antibodies and Proteomics Enzyme-Linked Immunosorbent Assay (ELISA)Antibody and Protein LabelingDirect ELISAPrimary Antibodies

Posted on June 1, 2020
Why streptavidin is better than avidin for binding biotin?
Antibodies and Proteomics Bioconjugation Enzyme-Linked Immunosorbent Assay (ELISA)BiotinBiotin and StreptavidinImmunohistochemistry (IHC)flow cytometryimmunofluorescence

Posted on June 1, 2020
What is chromatography and what are the types of chromatography?
Antibodies and ProteomicsAffinity ChromatographyChromatographic techniques

Posted on June 1, 2020
What is the difference between liquid chromatography (LC) and high-performance liquid chromatography (HPLC)?
Antibodies and ProteomicsAffinity ChromatographyChromatographic techniques

Posted on June 1, 2020
What is the difference between normal-phase chromatography and reversed-phase chromatography?
Antibodies and ProteomicsAffinity ChromatographyChromatographic techniques

Posted on June 1, 2020
How does reverse-phase chromatography separate proteins?
Antibodies and ProteomicsAffinity ChromatographyChromatographic techniques

Posted on June 1, 2020
Why is trifluoroacetic acid (TFA) used in reverse-phase chromatography for protein purification?
Antibodies and ProteomicsAffinity ChromatographyChromatographic techniquesProtein Purification

Posted on June 1, 2020
What are theoretical plates in HPLC?
Antibodies and ProteomicsAffinity ChromatographyChromatographic techniquesProtein Purification

Posted on June 1, 2020
What is spillover in flow cytometry?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingFlow Cytometry ReagentsFluorescenceSpectral Flow Cytometryflow cytometry

Posted on June 1, 2020
What is endotoxin?
Antibodies and ProteomicsGram-negativebacterialipid

Posted on June 1, 2020
How is endotoxin tested?
Antibodies and ProteomicsGram-negativebacterialipid

Posted on June 1, 2020
What is size-exclusion chromatography?
Antibodies and ProteomicsAffinity ChromatographyChromatographic techniquesProtein Purification

Posted on June 1, 2020
What are the exclusion limit and permeation limit in size-exclusion chromatography (SEC)?
Antibodies and ProteomicsAffinity ChromatographyChromatographic techniquesProtein Purification

Posted on June 1, 2020
What is ion-exchange chromatography (IXC)?
Antibodies and ProteomicsAffinity ChromatographyChromatographic techniquesProtein Purification

Posted on June 1, 2020
What are the types of ion-exchange resins?
Antibodies and Proteomics BioconjugationIntracellular Ions

Posted on June 1, 2020
What is the difference between ion exchange chromatography and affinity chromatography?
Antibodies and ProteomicsAffinity ChromatographyChromatographic techniquesProtein Purification

Posted on June 1, 2020
What kinds of interactions are used in affinity chromatography?
Antibodies and ProteomicsAffinity ChromatographyChromatographic techniquesProtein Purification

Posted on June 1, 2020
What is protein A chromatography?
Antibodies and ProteomicsAffinity ChromatographyChromatographic techniquesProtein Purification

Posted on June 1, 2020
How does a stacking gel work?
Antibodies and ProteomicsAgarose gel electrophoresisGel Electrophoresispolyacrylamide gel

Posted on June 1, 2020
What is the difference between SDS-PAGE and western blot?
Antibodies and ProteomicsGel ElectrophoresisProtein PurificationWestern Blot

Posted on May 18, 2020
What is the general protocol for analysis of surface expression of a CD marker using flow cytometry?
Antibodies and ProteomicsCluster of Differentiation (CD Antibodies)ProtocolSpectral Flow Cytometryflow cytometry

Posted on May 18, 2020
What is cell viability assay?
Antibodies and Proteomics Chemical ReagentsCell ViabilityCell Viability AssaysCellular ProcessesImmunohistochemistry (IHC)

Posted on May 18, 2020
What is cell sorting in flow cytometry?
Antibodies and ProteomicsCell ViabilityCell Viability AssaysCellular ProcessesFlow Cytometry ReagentsSpectral Flow Cytometryflow cytometry

Posted on May 18, 2020
Does Propidium Iodide specifically bind to dead cells?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCell Cycle AssaysCell Viability AssaysCellular Structures and OrganellesImmunohistochemistry (IHC)Propidium iodide (PI)

Posted on May 18, 2020
How do DNA binding dyes bind to DNA?
Antibodies and Proteomics Bioconjugation Chemical ReagentsAntibody and Protein LabelingCell Cycle AssaysCellular Structures and OrganellesDAPIImmunohistochemistry (IHC)Physiological ProbesPropidium iodide (PI)TUNEL

Posted on May 18, 2020
How is RNA isolated?
Antibodies and ProteomicsDNA and RNAExtractionFlow Cytometry ReagentsPhysiological ProbesPurificationRNASpectral Flow Cytometryflow cytometry

Posted on May 18, 2020
What is difference between therapeutic and prophylactic group in research?
Antibodies and Proteomics Chemical ReagentsImmunohistochemistry (IHC)

Posted on May 9, 2020
Which markers can be used to differentiate between human monocytes and macrophages?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCluster of Differentiation (CD Antibodies)Immunohistochemistry (IHC)flow cytometryimmunofluorescence

Posted on May 9, 2020
What markers can I use for investigating microglial activation in flow cytometry?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCluster of Differentiation (CD Antibodies)Flow Cytometry ReagentsIHCImmunohistochemistry (IHC)flow cytometry

Posted on May 9, 2020
What markers can I use for macrophages?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCluster of Differentiation (CD Antibodies)IHCImmunohistochemistry (IHC)

Posted on May 9, 2020
What markers can I use for neutrophils?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCluster of Differentiation (CD Antibodies)IHCImmunohistochemistry (IHC)

Posted on May 9, 2020
What are the best methods to identify necrotic cells?
Antibodies and ProteomicsApoptosisApoptosis and NecrosisCell ViabilityCell Viability AssaysCellular ProcessesDehydrogenaseEnzymesFlow Cytometry Reagentsflow cytometry

Posted on May 9, 2020
What are the best markers to define mesenchymal stromal cell (MSC) phenotype by FACS?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCluster of Differentiation (CD Antibodies)Immunohistochemistry (IHC)

Posted on May 9, 2020
What are the activation markers of mouse dendritic cells?
Antibodies and Proteomics BioconjugationCluster of Differentiation (CD Antibodies)Immunohistochemistry (IHC)

Posted on May 9, 2020
What does FACS backgating show?
Antibodies and ProteomicsCellular ProcessesPhysiological ProbesSpectral Flow Cytometryflow cytometry

Posted on May 9, 2020
Is there a spectral overlap between PerCP-Cy5.5 and PE-Cy5?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCluster of Differentiation (CD Antibodies)Flow Cytometry ReagentsImmunohistochemistry (IHC)PE and APCSpectral Flow Cytometry

Posted on May 9, 2020
What antibody concentration is recommended for intracellular staining using flow cytometry?
Antibodies and ProteomicsAntibody and Protein LabelingFlow Cytometry ReagentsIntracellular ProbesSpectral Flow Cytometryflow cytometry

Posted on May 9, 2020
Is mouse CD16/CD32 a marker for any particular cell type?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCluster of Differentiation (CD Antibodies)IHCImmunohistochemistry (IHC)

Posted on May 9, 2020
Can CD31 also be used as a marker for platelets instead of CD41?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCluster of Differentiation (CD Antibodies)IHCImmunohistochemistry (IHC)

Posted on May 9, 2020
How can one distinguish naive from effector/effector memory CD8 T cells?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCluster of Differentiation (CD Antibodies)IHCImmunohistochemistry (IHC)

Posted on May 9, 2020
What is the difference between nuclease-free water and ultrapure water?
Antibodies and Proteomics Chemical ReagentsDNA and RNANucleic Acids

Posted on May 9, 2020
What is the best method for permeabilizing cells?
Antibodies and Proteomics Chemical ReagentsBuffer Preparations and RecipesCellular Structures and OrganellesPBSPermeabilizationbuffercell lysisdetergent

Posted on May 9, 2020
What is the best way to store a buffy coat?
Antibodies and ProteomicsDNADNA extractionIHCImmunohistochemistry (IHC)Storage

Posted on May 9, 2020
Is it possible to use DAPI and PI simultaneously?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingDAPIFluorescenceImmunohistochemistry (IHC)Propidium iodide (PI)

Posted on May 9, 2020
What are PCR primers?
Antibodies and ProteomicsDNA and RNAPCRPCR primerPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)qPCR

Posted on May 9, 2020
What are reverse transcription primers?
Antibodies and Proteomics Peptide and Oligonucleotide LabelingCellular ProcessesDNA and RNADNA and RNA QuantitationPeptide and OligonucleotideRNAPCR primer

Posted on May 9, 2020
What are natural killer cells?
Antibodies and ProteomicsAntibody and Protein LabelingIHCImmunohistochemistry (IHC)fluorescence microscopeimmunofluorescence

Posted on May 9, 2020
How do natural killer cells function?
Antibodies and ProteomicsAntibody and Protein LabelingIHCImmunohistochemistry (IHC)

Posted on May 9, 2020
How can I analyze natural killer cells using flow cytometry?
Antibodies and ProteomicsAntibody and Protein LabelingCluster of Differentiation (CD Antibodies)Flow Cytometry ReagentsIHCImmunohistochemistry (IHC)Spectral Flow Cytometryflow cytometry

Posted on April 24, 2020
How can I determine if a protein has been knocked out?
Antibodies and ProteomicsProteinReal-Time PCR (qPCR)Western BlotqPCR

Posted on April 24, 2020
Any suggestions on how to insert antibodies into living cells?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingImmunohistochemistry (IHC)

Posted on April 24, 2020
How does the structure of the variable domain of an antibody allow it to bind with high affinity to its epitope?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingImmunohistochemistry (IHC)

Posted on April 24, 2020
What is the difference between mycotoxins and bacterial toxins?
Antibodies and Proteomics BioconjugationProteinbacteria

Posted on April 24, 2020
What can I do if low molecular weight proteins are not showing any bands in western blot?
Antibodies and ProteomicsAntibody and Protein LabelingGel ElectrophoresisProteinWestern Blot

Posted on April 24, 2020
Can polyclonal antibodies that are generated against tagged proteins cross-react?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingImmunohistochemistry (IHC)Protein

Posted on April 24, 2020
How do plants resist disease without antibodies?
Antibodies and ProteomicsCell Cycle AnalysisCellular ProcessesProteinplant cells

Posted on April 24, 2020
What are some methods for conjugation of antibodies with metallic nanoparticles?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingConjugationImmunohistochemistry (IHC)antibody conjugation

Posted on April 24, 2020
What is the difference between polyclonal and monoclonal antibodies?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCluster of Differentiation (CD Antibodies)IHCImmunohistochemistry (IHC)Protein

Posted on April 24, 2020
Why are there different antibody isotypes in our immune system?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingIHCIgGImmunohistochemistry (IHC)Physiological Probes

Posted on April 24, 2020
What is the difference between capture antibody and marker-antigen?
Antibodies and Proteomics Bioconjugation Enzyme-Linked Immunosorbent Assay (ELISA)Antibody and Protein LabelingELISAIHCImmunohistochemistry (IHC)

Posted on April 24, 2020
Why might polyclonal antibodies give better results than monoclonal antibodies?
Antibodies and Proteomics Bioconjugation Chemical ReagentsAntibody and Protein LabelingIHCImmunohistochemistry (IHC)Physiological Probes

Posted on April 24, 2020
What is the difference between reactogenicity and the immunogenicity of a vaccine?
Antibodies and ProteomicsCellular ProcessesCellular Structures and OrganellesImmunohistochemistry (IHC)

Posted on April 24, 2020
How can I increase the expression level of a protein?
Antibodies and ProteomicsAffinity ChromatographyAntibody and Protein LabelingChromatographic techniquesExtractionProteinProtein Purification

Posted on April 24, 2020
What is the difference between indirect and direct imaging?
Antibodies and ProteomicsAntibody and Protein LabelingIHCImmunohistochemistry (IHC)Primary AntibodiesSecondary Antibodiesimmunofluorescence

Posted on April 24, 2020
What is the difference between BCA and Micro BCA assay?
Antibodies and ProteomicsAffinity ChromatographyBCA assayBradford assayChromatographic techniquesExtractionProteinProtein PurificationProtein concentration

Posted on April 24, 2020
What is the difference between recombinant and synthetic polypeptides?
Antibodies and Proteomics BioconjugationDNA and RNAPeptide and OligonucleotideProtein

Posted on April 24, 2020
What is the difference between silica gels and bare silica?
Antibodies and Proteomics Bioconjugation Chemical ReagentsAffinity ChromatographyChromatographic techniques

Posted on April 24, 2020
Why do we use geometric mean concentration for antibody titer?
Antibodies and ProteomicsCalculating Concentration

Posted on April 24, 2020
What is the difference between Zymography and SDS-PAGE?
Antibodies and ProteomicsCellular ProcessesCellular Structures and OrganellesEnzymesProtein

Posted on April 24, 2020
Are there any broad-spectrum anti-bacterium antibodies?
Antibodies and ProteomicsCell SignalingGram StainingGram-negativeGram-positivebacteria

Posted on April 24, 2020
What is the major difference between Northern and Southern blotting?
Antibodies and ProteomicsAgarose gel electrophoresisDNA and RNAEnzymesGel ElectrophoresisNucleic AcidsProteinWestern Blotpolyacrylamide gel

Posted on April 24, 2020
What is the difference between western blotting and far-western blotting?
Antibodies and ProteomicsAgarose gel electrophoresisAntibody and Protein LabelingEnzymesGel ElectrophoresisProteinagarose gelpolyacrylamide gel

Posted on April 24, 2020
What is an appropriate incubation time for primary antibody in western blot analysis?
Antibodies and ProteomicsAgarose gel electrophoresisAntibody and Protein LabelingGel ElectrophoresisWestern Blotpolyacrylamide gel

Posted on April 24, 2020
Can I use an HRP-conjugated primary antibody for western blot (with no secondary)?
Antibodies and Proteomics Bioconjugation Chemical ReagentsAntibody and Protein LabelingHRPHRP-labeled primary antibodyHorseradish Peroxidase (HRP) and Poly-HRPPrimary AntibodiesSecondary AntibodiesWestern Blotantibody conjugation

Posted on April 24, 2020
What are the criteria for choosing primary and secondary antibodies in western blotting?
Antibodies and ProteomicsAntibody and Protein LabelingPrimary AntibodiesSecondary AntibodiesWestern Blot

Posted on April 24, 2020
Why does a secondary antibody have to originate from a host species different than that of the primary antibody?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingImmunohistochemistry (IHC)Primary AntibodiesSecondary Antibodies

Posted on April 24, 2020
What is the difference between anti-human and anti-mouse antibodies?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCluster of Differentiation (CD Antibodies)Immunohistochemistry (IHC)antibody conjugation

Posted on April 24, 2020
Can I use unstained cells as negative controls for FACS analysis?
Antibodies and Proteomics BioconjugationPrimary Antibodiesantibody conjugation

Posted on April 24, 2020
What is the right DAPI concentration to use in a flow cytometry experiment?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCell Cycle AssaysCell Structure and OrganellesCellular ProcessesDAPIFlow Cytometry ReagentsSpectral Flow Cytometry

Posted on April 24, 2020
What is the difference between a serotype and a subtype of a virus?
Antibodies and Proteomics Enzyme-Linked Immunosorbent Assay (ELISA)Antibody and Protein LabelingDNA and RNAEnzymesProtein

Posted on April 24, 2020
Can a human hormone assay kit be used for rats and mice?
Antibodies and Proteomics BioconjugationAntibody and Protein Labeling

Posted on April 24, 2020
What is the difference between HLA-DR and Iba1?
Antibodies and Proteomics BioconjugationIHCImmunohistochemistry (IHC)Protein

Posted on April 24, 2020
Which method is better for measuring the amount of antibodies in a sample, ELISA or FACS?
Antibodies and Proteomics Enzyme-Linked Immunosorbent Assay (ELISA)ELISAEnzymesPeptide and Oligonucleotide

Posted on April 24, 2020
What is the difference between agroinfiltration and agroinfection?
Antibodies and ProteomicsCell CultureCellular Structures and OrganellesDNAplant cells

Posted on April 24, 2020
What is in antiserum?
Antibodies and Proteomics Bioconjugation Chemical ReagentsProtein

Posted on April 24, 2020
To generate antibodies in mice, should I use intraperitoneal or subcutaneous injection?
Antibodies and Proteomics BioconjugationAntibody and Protein Labeling

Posted on April 24, 2020
Can antibodies for flow cytometry be used for fluorescence microscopy?
Antibodies and ProteomicsFluorescenceSpectral Flow Cytometryflow cytometryfluorescence microscope

Posted on April 24, 2020
Why is TMB used in conjunction with HRP in ELISA?
Antibodies and Proteomics Bioconjugation Enzyme-Linked Immunosorbent Assay (ELISA)Antibody and Protein LabelingHRPHorseradish Peroxidase (HRP) and Poly-HRPImmunohistochemistry (IHC)

Posted on April 24, 2020
What is the difference between western blotting and immunoprecipitation?
Antibodies and ProteomicsGel ElectrophoresisProteinWestern Blot

Posted on April 24, 2020
Is there a good antibody available to detect C-terminal fragments of amyloid precursor protein (APP)?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingIHCImmunohistochemistry (IHC)

Posted on April 24, 2020
Does von Willebrand factor induce platelet aggregation or agglutination?
Antibodies and ProteomicsImmunohistochemistry (IHC)

Posted on April 24, 2020
What is the difference between Myc and c-Myc tag proteins?
Antibodies and Proteomics Enzyme-Linked Immunosorbent Assay (ELISA)Antibody and Protein LabelingImmunohistochemistry (IHC)ProteinWestern Blot

Posted on April 24, 2020
What is the difference between M1 and M2 macrophages and how can you differentiate them?
Antibodies and ProteomicsCD80Cell Cycle AnalysisCell Cycle and ProliferationCellular ProcessesCluster of Differentiation (CD Antibodies)Immunohistochemistry (IHC)

Posted on April 24, 2020
What is the right concentration of antibodies to use when labelling with fluorescent dyes?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingImmunohistochemistry (IHC)

Posted on April 17, 2020
What is the function of adding BSA as buffer in Biotin-Streptavidin system?
Antibodies and Proteomics Bioconjugation Chemical ReagentsAntibody and Protein LabelingBSABiotin and StreptavidinBuffer Preparations and Recipesbovine serum albuminbuffer

Posted on April 17, 2020
What is the benefit of using TN buffer (10 mM Tris, 400 mM Nacl, pH 7.5) in the RNA extraction?
Antibodies and Proteomics Chemical ReagentsBuffer Preparations and RecipesDNA and RNAExtractionRNAbuffer

Posted on April 17, 2020
What is the actual mechanism involved in blocking a nitrocelluolse membrane ?
Antibodies and Proteomics Bioconjugation Chemical ReagentsBSABuffer Preparations and Recipesbovine serum albuminbuffer

Posted on April 17, 2020
Why is 100% Ethanol used in rehydration of paraffin embedded tissues?
Antibodies and Proteomics Chemical ReagentsAntibody and Protein LabelingIHCImmunohistochemistry (IHC)Paraffin embedded sectionsProtein

Posted on April 17, 2020
In IHC what should be incubation time for primary antibody and secondary antibody ?
Antibodies and ProteomicsAntibody and Protein LabelingImmunohistochemistry (IHC)Primary AntibodiesSecondary Antibodies

Posted on April 17, 2020
How to circumvent the non specific binding of monoclonal Antibody?
Antibodies and ProteomicsAntibody and Protein LabelingCluster of Differentiation (CD Antibodies)Immunohistochemistry (IHC)

Posted on April 17, 2020
Does adding triton with the primary antibody hinder antigen detection in immunohistochemistry?
Antibodies and ProteomicsAntibody and Protein LabelingImmunohistochemistry (IHC)Primary Antibodies Chemical ReagentsBuffer Preparations and RecipesIHCbufferdetergent

Posted on April 17, 2020
What is the reason for positive staining in negative control tissue during Immunohistochemistry (IHC)?
Antibodies and ProteomicsAntibody and Protein LabelingImmunohistochemistry (IHC)IHChydrogen peroxide

Posted on April 17, 2020
What are the advantages and disadvantages of varying incubation time and temperature for immunofluorescence staining of tissue?
Antibodies and ProteomicsAntibody and Protein LabelingImmunohistochemistry (IHC)immunofluorescenceIHC

Posted on April 17, 2020
Why do we use Citrate buffer in Antigen retrieval step during IHC?
Antibodies and ProteomicsAntibody and Protein LabelingImmunohistochemistry (IHC)buffer Chemical ReagentsBuffer Preparations and Recipes

Posted on April 17, 2020
What is the detailed mechanism of PMA differentiation of U937 cells?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingIHCImmunohistochemistry (IHC)

Posted on April 17, 2020
When should DTT be added in tissue lysis?
Antibodies and Proteomics Chemical ReagentsBuffer Preparations and RecipesPhysiological Probesbuffer

Posted on April 17, 2020
Why is the function of E-Cadherin totally different from N-Cadherin?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingIHCImmunohistochemistry (IHC)

Posted on April 17, 2020
Can we co-incubate the primary and secondary antibodies at the same time for Western Blotting ?
Antibodies and ProteomicsAntibody and Protein LabelingPrimary AntibodiesSecondary AntibodiesWestern Blot

Posted on April 17, 2020
Should I use cold or warm PBS washes when performing protein extraction?
Buffer Preparations and RecipesPBSbuffer Antibodies and ProteomicsEnzymesExtractionProtein

Posted on April 17, 2020
What is blocking buffer in IHC?
Antibodies and ProteomicsBSAImmunohistochemistry (IHC)PBSbuffer Chemical ReagentsIHC

Posted on April 17, 2020
How to quench glutaraldehyde fluorescence?
BioconjugationFluorescenceautofluorescence Antibodies and ProteomicsAntibody and Protein LabelingPhysiological Probes

Posted on April 17, 2020
What is the role of Tween-20 in antigen retrieval buffer?
Immunohistochemistry (IHC)detergent Antibodies and Proteomics Chemical ReagentsBuffer Preparations and RecipesIHCbuffer

Posted on April 17, 2020
Is Gelatin or BSA best for blocking in immunofluorescence?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingBSAimmunofluorescence Chemical Reagents

Posted on April 17, 2020
What is the role of sodium azide in flow cytometry?
Antibodies and Proteomics Chemical ReagentsFlow Cytometry ReagentsFluorescencePhysiological ProbesSpectral Flow Cytometry

Posted on April 17, 2020
What would be the best marker in IHC/IF for bronchial epithelial cells?
Antibodies and ProteomicsAntibody and Protein LabelingImmunohistochemistry (IHC)immunofluorescence

Posted on April 17, 2020
What is the advantage or dissadvantage of having Tween 20 in flow cytometry wash and staining buffers?
Antibodies and Proteomics Chemical ReagentsBuffer Preparations and RecipesFlow Cytometry ReagentsSpectral Flow Cytometrybufferdetergent

Posted on April 17, 2020
What's the role of BSA/Milk as blocking agents??
Antibodies and Proteomics Bioconjugation Enzyme-Linked Immunosorbent Assay (ELISA)Antibody and Protein LabelingBSABuffer Preparations and RecipesImmunohistochemistry (IHC)bovine serum albuminbuffer

Posted on April 17, 2020
What is the role of Tau protein aggregation in neurodegeneration?
Antibodies and Proteomics Bioconjugation Enzyme-Linked Immunosorbent Assay (ELISA)Antibody and Protein LabelingWestern Blot

Posted on April 17, 2020
What are some reliable methods for validating antibodies?
Antibodies and Proteomics Enzyme-Linked Immunosorbent Assay (ELISA)Antibody and Protein LabelingIHCImmunohistochemistry (IHC)Western Blot

Posted on April 17, 2020
Is agitation necessary during IHC antibody incubation?
Antibodies and ProteomicsBuffer Preparations and RecipesIHCImmunohistochemistry (IHC)Permeabilizationbufferdetergent

Posted on April 17, 2020
What are the differences between stable and permanent transfection/transduction?
Antibodies and Proteomics Chemical ReagentsDNA and RNANucleic AcidsPhysiological ProbesTransfectionTransfection Reagents

Posted on April 17, 2020
What % of BSA to use for blocking step?
Antibodies and Proteomics Chemical ReagentsBSABuffer Preparations and RecipesPhysiological Probesbovine serum albuminbuffer

Posted on April 2, 2020
How many clusters of differentiation are there?
Antibodies and ProteomicsAntibody and Protein LabelingCluster of Differentiation (CD Antibodies)Immunohistochemistry (IHC)

Posted on April 2, 2020
What is the difference between CD4 and CD8 T cells?
Antibodies and ProteomicsAntibody and Protein LabelingCluster of Differentiation (CD Antibodies)Immunohistochemistry (IHC)IHC

Posted on April 2, 2020
What is the role of CD45 in T cell activation?
Antibodies and ProteomicsAntibody and Protein LabelingCluster of Differentiation (CD Antibodies)Immunohistochemistry (IHC)IHC

Posted on April 2, 2020
What is CD45 a marker for?
Antibodies and ProteomicsAntibody and Protein LabelingCluster of Differentiation (CD Antibodies)Immunohistochemistry (IHC)IHC

Posted on April 2, 2020
What is the significance of cluster differentiation CD markers in immunophenotyping?
Antibodies and ProteomicsAntibody and Protein LabelingCluster of Differentiation (CD Antibodies)Immunohistochemistry (IHC)

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